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Shc2 (NM_001024539) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG215808
Shc2 (tGFP-tagged) - Mouse SHC (Src homology 2 domain containing) transforming protein 2 (Shc2), (10ug)
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ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $733.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Shc2
Synonyms 6720466E06; SCK; ShcB; Sli
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG215808 representing NM_001024539
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGACGCAGGGTCCGGGTGGCCGCGCGGCCCCCGAGCCCGAGGCGCCCACCACCTTCTGCGCACTGCTGC
CGCGGATGCCGCAGTGGAAGTTCGCAGCGCCGGGTAGCTTCCTGGGCCGCGGTCCCGCGGCGACGCGCGT
GGCGGGGGTGGCAGAGGCGCAGCCGGAGCCCGGGGTCCCCGCGCTGGCCGCTGTGTTGGGCGCTTGCGAA
CCGCGCTGCGCCGCGCCCTGTCCGCTGCCCGCGCTCGGCCGCTGCCGGGGCTCGGGGACGCGGGGCGCGC
GGGTGACTCCGGATGTCGCTGACGAGTGGGTGCGCAAGGGCGGCTTCATTCACAAGCCGGCGCACGGCTG
GTTGCATCCCGATGCCAGGGTCCTGGGGCCCGGGGTCTCTTACATCGTTCGGTACATGGGCTGCATTGAG
GTGCTTCGATCCATGCGCTCCCTGGATTTCAACACCCGAACACAGGTGACGAGGGAAGCCATCAATCGGC
TCCATGAGGCTGTGCCCGGTGTCCGGGGCTCCTGGAAGAAGAAGGCCCCCAACAAGGCTCTGGCCTCCAT
CTTGGGGAAAAGCAACCTGCGCTTCGCCGGCATGAGCATCTCAGTCAACATCTCCGTGGACGGCCTTAAC
TTGTCTGTTCCCGCCACCCGCCAGATCATCGCCAACCATCATATGCAGTCTATTTCCTTCGCCTCGGGTG
GTGACACGGACATGACTGATTACGTGGCCTATGTGGCCAAGGACCCCATCAACCAGAGAGCCTGCCACAT
CTTGGAATGCTGTGAAGGTCTTGCCCAGAGCGTCATCAGCACCGTAGGGCAAGCCTTTGAGCTGCGCTTC
AAGCAATACCTGCACAGTCCGCCCAAGGCGGTAGTGCCCCCTGAAAGGCTGACTGGGCTGGAGGAGTTGG
CCTGGGGAGATGATGACGCTGCTGCAGACCACAATTACTACAACAGCATTCCGGGAAAGGAGCCACCCCT
GGGCGGGCTGGTGGACTCCAGACTGGCTGTCACACAGCCCTGTGCGCTGGCGACACTCGGGGGCCTTGGA
CAGGGAATGACACCAGTATGGAGAGATGCCCGTGGCTTGCCTTGGGACATGGGCCCCTCTGGAGCAGCCC
CACCGGGGGATGGCTACGTGCAGGCAGATGCCCGAGGGCCACATGACTACGAGGAGCACCTGTATGTCAA
TACCCAGGGCCTGGATGCTGTGGAGCTTGAGGACACCGCCGAGGCACCTCTGCAGTTTGAGGACAGCCCC
AAGAAGGACCTGTTTGACATGCGACCCTTTGAAGATGCCCTGAAGTTGCACGCGTGCTCAGTGGCAGCCG
GCATCACTGCAGCCTCACCCCCTCTGGAAGACCAGTGGCCCAGTCCCCCTACCCGCAGGGCCCCCATTGC
ACCCACAGAGGAGCAATTGAGGCAGGAGCCCTGGTACCACGGACGAATGAGCCGTCGGGCTGCAGAGAAG
CTGCTTCGGGCCGATGGGGACTTCCTTGTGAGAGACAGCGTCACCAACCCGGGGCAGTATGTCCTCACGG
GCATGCATGCGGGGCAGCCCAAGCACCTGCTGCTGGTGGACCCCGAGGGTGTGGTGCGGACGAAAGATGT
GTTGTTTGAGAGCATCAGCCACCTCATAGACTATCACCTGAAGAATGGGCTGCCTATCGTGGCTGCTGAG
AGCGAGCTGCATCTGCGGGGAGTGGTCTCTCGGGAGCCA

AGCGGACCGACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG215808 representing NM_001024539
Red=Cloning site Green=Tags(s)

MTQGPGGRAAPEPEAPTTFCALLPRMPQWKFAAPGSFLGRGPAATRVAGVAEAQPEPGVPALAAVLGACE
PRCAAPCPLPALGRCRGSGTRGARVTPDVADEWVRKGGFIHKPAHGWLHPDARVLGPGVSYIVRYMGCIE
VLRSMRSLDFNTRTQVTREAINRLHEAVPGVRGSWKKKAPNKALASILGKSNLRFAGMSISVNISVDGLN
LSVPATRQIIANHHMQSISFASGGDTDMTDYVAYVAKDPINQRACHILECCEGLAQSVISTVGQAFELRF
KQYLHSPPKAVVPPERLTGLEELAWGDDDAAADHNYYNSIPGKEPPLGGLVDSRLAVTQPCALATLGGLG
QGMTPVWRDARGLPWDMGPSGAAPPGDGYVQADARGPHDYEEHLYVNTQGLDAVELEDTAEAPLQFEDSP
KKDLFDMRPFEDALKLHACSVAAGITAASPPLEDQWPSPPTRRAPIAPTEEQLRQEPWYHGRMSRRAAEK
LLRADGDFLVRDSVTNPGQYVLTGMHAGQPKHLLLVDPEGVVRTKDVLFESISHLIDYHLKNGLPIVAAE
SELHLRGVVSREP

SGPTRTRRLE - GFP Tag - V
Restriction Sites SgfI-RsrII Cloning Scheme for this gene | Plasmid Map
ACCN NM_001024539
ORF Size 1719 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001024539.1, NP_001019710.1
RefSeq Size 3974 bp
RefSeq ORF 1722 bp
Locus ID 216148
UniProt ID Q8BMC3
Cytogenetics 10 39.72 cM
Summary Signaling adapter that couples activated growth factor receptors to signaling pathway in neurons. Involved in the signal transduction pathways of neurotrophin-activated Trk receptors in cortical neurons.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_001024539" in other vectors (4)
SKU Description Size Price
MC219220 Shc2 (untagged) - Mouse SHC (Src homology 2 domain containing) transforming protein 2 (Shc2), (10ug) 10 ug
$588.00
MR215808 Shc2 (Myc-DDK-tagged) - Mouse SHC (Src homology 2 domain containing) transforming protein 2 (Shc2) 10 ug
$289.00 $533.00
MR215808L3 Lenti ORF clone of Shc2 (Myc-DDK-tagged) - Mouse SHC (Src homology 2 domain containing) transforming protein 2 (Shc2) 10 ug
$833.00
MR215808L4 Lenti ORF clone of Shc2 (mGFP-tagged) - Mouse SHC (Src homology 2 domain containing) transforming protein 2 (Shc2) 10 ug
$833.00

file-alt Citations

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