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Ptges3 (NM_019766) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG215806
Ptges3 (tGFP-tagged) - Mouse prostaglandin E synthase 3 (cytosolic) (Ptges3), (10ug)
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ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $350.00
3 Weeks*
Bulk Requests & Clone Modifications

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Other products for "Ptges3 or NM_019766"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Ptges3
Synonyms 5730442A20Rik; cPGES; p23; Ptges; sid3177; Tebp
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG215806 representing NM_019766
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCAGCCTGCTTCTGCAAAGTGGTACGATCGAAGGGACTATGTATTCATTGAATTTTGTGTTGAAGACA
GTAAAGATGTTAATGTAAACTTTGAAAAATCCAAACTTACTTTCAGTTGTCTTGGAGGAAGCGATAATTT
TAAGCATTTAAATGAAATTGATCTTTTTCATTGTATCGATCCAAATGATTCCAAGCATAAAAGAACAGAC
AGATCGATTTTATGTTGTTTGCGAAAAGGAGAATCCGGCCAGTCATGGCCTAGGTTAACAAAGGAAAGGG
CAAAGCTTAATTGGCTCAGTGTGGACTTCAATAATTGGAAAGACTGGGAGGATGACTCAGATGAAGACAT
GTCGAATTTTGACCGTTTCTCTGAGATGATGGATCACATGGGTGGTGATGAGGATGTAGATTTACCAGAA
GTAGATGGAGCAGATGATGATTCACAAGACAGTGATGATGAAAAGATGCCAGATCTGGAG

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG215806 representing NM_019766
Red=Cloning site Green=Tags(s)

MQPASAKWYDRRDYVFIEFCVEDSKDVNVNFEKSKLTFSCLGGSDNFKHLNEIDLFHCIDPNDSKHKRTD
RSILCCLRKGESGQSWPRLTKERAKLNWLSVDFNNWKDWEDDSDEDMSNFDRFSEMMDHMGGDEDVDLPE
VDGADDDSQDSDDEKMPDLE

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_019766
ORF Size 480 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_019766.4, NP_062740.1
RefSeq Size 1954 bp
RefSeq ORF 483 bp
Locus ID 56351
UniProt ID Q9R0Q7
Cytogenetics 10 D3
Summary Cytosolic prostaglandin synthase that catalyzes the oxidoreduction of prostaglandin endoperoxide H2 (PGH2) to prostaglandin E2 (PGE2). Molecular chaperone that localizes to genomic response elements in a hormone-dependent manner and disrupts receptor-mediated transcriptional activation, by promoting disassembly of transcriptional regulatory complexes. Facilitates HIF alpha proteins hydroxylation via interaction with EGLN1/PHD2, leading to recruit EGLN1/PHD2 to the HSP90 pathway.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_019766" in other vectors (4)
SKU Description Size Price
MC210042 Ptges3 (untagged) - Mouse prostaglandin E synthase 3 (cytosolic) (Ptges3), (10ug) 10 ug
$165.00
MR215806 Ptges3 (Myc-DDK-tagged) - Mouse prostaglandin E synthase 3 (cytosolic) (Ptges3) 10 ug
$289.00
MR215806L3 Lenti ORF clone of Ptges3 (Myc-DDK-tagged) - Mouse prostaglandin E synthase 3 (cytosolic) (Ptges3) 10 ug
$450.00
MR215806L4 Lenti ORF clone of Ptges3 (mGFP-tagged) - Mouse prostaglandin E synthase 3 (cytosolic) (Ptges3) 10 ug
$450.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.