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Efhb (NM_172497) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG215793
Efhb (tGFP-tagged) - Mouse EF hand domain family member B (Efhb), (10ug)
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ORF Plasmid:
tGFP DDK
  $981.00
3 Weeks*
Bulk Requests & Clone Modifications

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Other products for "Efhb or NM_172497"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Efhb
Synonyms 4921525D22Rik
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG215793 representing NM_172497
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTGTTCGTTTGTGAGAGTGGGGTCCCCCAAACCTTTGCAAACTTCAGCTTCTCCGTTAGAGATGAGTT
CCCTGAGAAGGACGAGAGCACCTGAAATCTCGGAACTTGGACTCACCCCAGAGCAAAAGGACGATATAAG
AGACAGGGTACTTAGGGGGTCAAAATCTCCCACGGAGCTGGGGTTTGACTTGAGACTAGAACAAGATCGA
AAATGGAGAGAGAGGATGGGATCATCAGAAGCAAAATCGCCACCTTGCCATGCTCTTGGAGTAGGATTAG
AAAGACATACTATTTCTGGGACACCCACAGAGATGGGTAATTTAGGACTTCACAAAGGCTCTGCCTTCCA
GGGATCAAAACCTCTGGGTGTGTTACCAGGAAGAGTGGGGCCAGAAAACAAGGGCCTTCCGCCTCGCCTT
CGCTACGGAGGGACACTACACCCTCCTTTCAGCACAGTACATGCAAGTCCGCTGGCGGCAGAGAGCAGAA
GGAGGCCTTTAGCCTGGGGTTCTGCTTGGACGGATGCTGTAGTGGAAAAGCAACCTGTTGTAGGTTTGGA
ACTGAGAAAAGAACCGGAAAAAGAACCCACCTGTGTCGTGATGAACCCCTACCCAGAGATGCCCCCTAAG
GAGGTAGACATCGGGTTGCCACAAACTCAGGAATCAGATGAAGCCAAGAATACAGAGCCTCTAATAGGTT
TAGTAAGAGAACCTTCTGAATGTCCATTTGCCCAACAACCGGAAGAGAAAAAGGAGCCCGGCAGCACCGA
ACCCGGAGTGGAGCCTCCAGGGAACATCAGGCCCATATACTCAGGGAAGTTCTTTGATCGGGTGCCTTGC
TGGCCAAGTGCAGGAAAAGTTAAGCCAGTTGGATACAGAGTTGCAACCTGTTTGACTGAGAAGCTTCCCA
GGCTAATGACTCCCCCAGAGGCAAAGAAGTATTTCAACTTCAGATATCCACCTGCTGGAGCAGAAAGAGT
ATTTTATGGCAGAGCAAATGACCCCCAAATTGCACCTTATTTGACACATGGACTAAGATCTAAAATCTCA
ATACCGATGGGATCATTGATAAACCCACAGCCCATTACCACGTTTCAACAGAAAATTAAAGACAAAAAAG
AATCTATATATTTTAGCCATCAACGAGCACCATTGGGAAAATCTCATGATCAAACACCAGGATTGCCAAA
AGGAATGGATGTGATCAACACAACATTAGGGACACCGACTATCAGAGAACTGTCTGTTCGTGACACTGTG
AATCCATCGAAATCCTTCGAAGATGTGCTTAAAGAAGGACAGGAAGGACATGATTTGTACACTGTCTCTC
ACAATGATTATTTTGCAGGAGAAGCAAAGAATCGAAAATATAACCCGGCAAGCTTCCATAGGTTTAACTT
GTATGGAATCCCAACACCTCACTTTAATGATGGGCGCACCATGGCTAAAGCTTTACATTGGCTCCATGAA
CTACAAATGGAAAGAGGAGCGAAAATTGTATCCAAAAGAGTTGATGACTTCAAAGAGAAATTTCAACACA
AACTTGGAAAAGTTTTAGACCCCATTGCAGAAACTATGAATGTTCCCCCAGGTCACACATTTGGAAGTTG
CCTCCATCCTGAGGAATATGGAGCTGGCGATCTCATCCATTATAGAAGCCCAGATGAATATCTTCGTGGG
AAGGACCACCAGAGAGCCGTGGTTGCAGCTGCTCGCCACCACTTGAAGAAATTTAATCACCAGAACTTTG
ACACTTTGCAGGTGGCTTTCAGGCACTATGACAAGAAGGGAGATGGGGTGATTGACAGAGCAGAACTTCA
TGAAGCTTGTGTCCAAGCCAACCTGCATCTAGACAAGATGCTCCTGGACCATCTGTTTGACTACTGTGAC
GTGGACCAGGATGGCTTGATCAACTACCTGGAATTTGCCAATTTCCTTAACTGGAAAGACAGAATACCTC
TCAAAGAGCATGAGAAGAGGGTCGTTGTTAAAGGTAAAAAACCAGACTGTGAAAACGTTACTGATACTAG
CATGGGAGAAGCTGAACCAAGTCTCCTGATAAATCCTGAAGACATTGTCCCCAAAGAACCAGGAAGTTCA
GAAGAGACTCTCCGGACAATCCAGAGACCAGGTGACAAAGTTTCTCACCAGTATAAGACAACGTCTTCTG
AGATCAACGCTGTGGTTGGAGCTGTCCCCTCCATGTGTCACCCCATATTCGGTGTTCCAACCATTCGATC
TGACATCTCTGCTCCCCGGATTCGTCGAGTCAGTGACATGAATAACTACGGTGACGAAGGCAATGCTTAC
TCTCTGCTGCACCCCTCCATCTTCTCCCAGAAAGGTGTATTTGAGAGAGACTTCTTCAAGACAAGGTCAA
AGGAAGAGATTTCAGATATATTAACTAACATTGGAGTCAAGCTTTCTAAGGAAGAATTCGAAAATGTGTG
GAATCTTGCTTCAAAAAAACACCAGCGAGGAGAAGTTTGTGTTGAAACTATCAGGAACGTTCTAGATGAG
CTACTGCATGCAGACCTCGTCAAGTGTAAAACGGCCATG

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG215793 representing NM_172497
Red=Cloning site Green=Tags(s)

MCSFVRVGSPKPLQTSASPLEMSSLRRTRAPEISELGLTPEQKDDIRDRVLRGSKSPTELGFDLRLEQDR
KWRERMGSSEAKSPPCHALGVGLERHTISGTPTEMGNLGLHKGSAFQGSKPLGVLPGRVGPENKGLPPRL
RYGGTLHPPFSTVHASPLAAESRRRPLAWGSAWTDAVVEKQPVVGLELRKEPEKEPTCVVMNPYPEMPPK
EVDIGLPQTQESDEAKNTEPLIGLVREPSECPFAQQPEEKKEPGSTEPGVEPPGNIRPIYSGKFFDRVPC
WPSAGKVKPVGYRVATCLTEKLPRLMTPPEAKKYFNFRYPPAGAERVFYGRANDPQIAPYLTHGLRSKIS
IPMGSLINPQPITTFQQKIKDKKESIYFSHQRAPLGKSHDQTPGLPKGMDVINTTLGTPTIRELSVRDTV
NPSKSFEDVLKEGQEGHDLYTVSHNDYFAGEAKNRKYNPASFHRFNLYGIPTPHFNDGRTMAKALHWLHE
LQMERGAKIVSKRVDDFKEKFQHKLGKVLDPIAETMNVPPGHTFGSCLHPEEYGAGDLIHYRSPDEYLRG
KDHQRAVVAAARHHLKKFNHQNFDTLQVAFRHYDKKGDGVIDRAELHEACVQANLHLDKMLLDHLFDYCD
VDQDGLINYLEFANFLNWKDRIPLKEHEKRVVVKGKKPDCENVTDTSMGEAEPSLLINPEDIVPKEPGSS
EETLRTIQRPGDKVSHQYKTTSSEINAVVGAVPSMCHPIFGVPTIRSDISAPRIRRVSDMNNYGDEGNAY
SLLHPSIFSQKGVFERDFFKTRSKEEISDILTNIGVKLSKEEFENVWNLASKKHQRGEVCVETIRNVLDE
LLHADLVKCKTAM

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_172497
ORF Size 2559 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_172497.3, NP_766085.2
RefSeq Size 2661 bp
RefSeq ORF 2562 bp
Locus ID 211482
UniProt ID Q8CDU5
Cytogenetics 17 C
Summary Cytosolic sensor for calcium, modulates the interaction of STIM1 and ORAI1 upon store depletion, the activation of store-operated Ca(2+) entry (SOCE) and NFAT translocation from cytosol to nucleus.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_172497" in other vectors (4)
SKU Description Size Price
MC222072 Efhb (untagged) - Mouse EF hand domain family, member B (Efhb), (10ug) 10 ug
$860.00
MR215793 Efhb (Myc-DDK-tagged) - Mouse EF hand domain family, member B (Efhb) 10 ug
$589.00 $781.00
MR215793L3 Lenti ORF clone of Efhb (Myc-DDK-tagged) - Mouse EF hand domain family, member B (Efhb) 10 ug
$1,081.00
MR215793L4 Lenti ORF clone of Efhb (mGFP-tagged) - Mouse EF hand domain family, member B (Efhb) 10 ug
$1,081.00

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.