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D5Ertd579e (NM_001081232) Mouse Tagged ORF Clone

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SKU
MG215792
D5Ertd579e (tGFP-tagged) - Mouse DNA segment Chr 5 ERATO Doi 579 expressed (D5Ertd579e), (10ug)
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ORF Plasmid:
tGFP DDK
  $1,394.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol D5Ertd579e
Synonyms 9030221A05Rik; A930018H20Rik; Kiaa0232
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG215792 representing NM_001081232
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCGCCCTGTCTGTACAGTTGTTGTGGATGGTTTGCCATCTGAAAGCACCTCAAGTTCTTATCCAGGCC
CTGTGTCTGTTTCTGACATGTCGCTGCTTCATGCATTGGGCCCAGTGCAAACCTGGCTGGGACAAGAGCT
GGAGAAATGTGGCATTGATGCCATGATTTATTCACGGTATATCCTCAGTCTTCTGCTGCATGACAGCTAT
GACTACGACCTGCAGGAACAGGAGAATGACATCTTGAGCTGGGAGAAAGGAGCTTATAAGAAATGGGGAA
GGAGTAAGAAGAAGTGTTCTGATCTAACGCTGGAGGAGATGAAAAAGCAGGCTGCTGTCCAGTGTCTGCG
CTCTGCTTCTGATGAAAGCTCTGGGATCGAGACTTTAGTAGAAGAGCTCTGCTGCAGACTGAAAGACCTA
CAGAGCGAGCAAGAAGAGAAGATTCACAAAAAGTTAGAAGGTTCTCCTTCTCCAGAGGAAGAATTGTCCC
CTACAGCAAAAGATCAAGTGGAAATGTACTATGAAGCATTTCCACCACTGTCTGAGAAACCGGTTTGTCT
GCAAGAGATCATGACCGTGTGGAACAAGTCCAAACCCTGTTCTTACTCTAGCTCCTCGTCATCATCCACA
GTCCCACCAGCAAGCACGGATACATCTTCTCCAAAGGACTGCAACAGTGAGAGTGAAGCCGTCAGAGAGA
GAAGCAGTGTCGCCTCTGTCCCCATGCATGAGAAAGCCCAGAGCAGGAGCAGACACGAGAAGGAGAGCAA
GCTGAGCAGTAGCACCATCGAAGAAAAGCCTGCCTTTTACAAAAGGCAGATCCGACACAAACCTGAAGGG
AAGACGCGCCCTCGCTCATGGTCATCTGGCTCTAGTGAAGCAGGTTCAAGTTCAAGTGGTAACCAAGGAG
AGTTAAAAGCATCCATGAAGTATGTTAAAGTCAGACACAAGGCACGAGAAATTCGAAACAGAAAAGGGCG
GAATGGGCAAAACAGGCATTCACTGAAGCATTGTGGAAAGGCAGAGAGAGGAGTCCATGCAGGCAGTGGT
GGCAGCAGCAGCAGCAGCAGCAATGGGTCCATCAGGCAGCTGTGCAAGCGGGGTAAAAGACCAGCAAAGG
AGACGGGAAGGAGCGACTCTGGGAACACTGCAGTGAAAGACCTGTATGTGGACAGCAGAAACAACAAAGA
GTACAAGGAAGAGCCCCTGTGGTACACAGAGCCCATTGCTGAGTATTTTGTTCCTTTGAGTAGAAAAAGC
AAACTAGAGACCACATACCGAAACAGGGAAGATACAAGTACTCTGACAGCAGAGGCAGTGGAAGACTTGT
CTGACTCTGTGCGTGGTCTGTGTATCAGTAACAGTAATATCCACAGAACATACCTCGCAGCAGGTACTTT
CATTGATGGTCATTTTGTAGAAATGCCTGCAGTTATAAACGAGGATATTGACCTCGCTGGGACCTCATTA
TGTTCTCTACCAGAGGACAATAAGTATCTGGATGATATTCATCTATCAGAACTAACACACTTCTATGAAG
TGGATATTGATCAATCCATGTTGGATCCTGGTGCCTCAGAAACAATGCAAGGAGAAAGTCGGATTTTGAA
TATGATTCGACAAAAAAGCAAGGAGAATACAGATTTTGAGGCAGAATGTTGCATAGTGTTAGATGGTATG
GAGTTGCAAGGGGAACGTGCAATATGGACAGATTCTACCAGCTCTGTGGGTGCTGAGGGCTTCTTCCTGC
AAGACCTTGGCAATCTGGCTCAGTTTTGGGAGTGTTGTTCATCTAGTTCTGGTGATGCTGATGGAGAGAG
TTTTGGAGGAGACTCTCCAGTTAGACTCTCTCCTATCTTGGACAGCACGATGCTCAGTTCACACATACTT
GCTGGCAATCAAGAGCCCTTTTCCAATATTAATGAAGGATCTGGTATAAACTCTTGTTTTTCAGTATTTG
AAGTGCAATGCAGTAATTCTGTTTTACCATTTTCTTTTGAAACACTCAACTTGGGAAGTGAACATGCAGA
TTCTAGTGCTAATATGCTGGGGAAAACACAGTCTAGATTGCTCATATGGACCAAAAATAGTGCCTTTGAA
GAAAACGAACACTGTTCTAATCTTTCAACAAGAACTTGTAGTCCGTGGTCCCATTCGGAAGAAGCACGCT
CAGACAATGAGACACTAAACATTCAGTTTGAAGAATCGACACAGTTTACTGCAGAAGATATTAATTATGT
GGTTCCCAGAGTCTCGTCAGATTTTGTAGATGAAGAGCTTCTAGATTTTTTGCAGGATGAAACTTGCCAG
CAAAACAGTAGAACTTTAGGAGAAATTCCTACATTAGTTTTCAAAAAACGATCTAAGCTAGAATCTGTGT
GTGGTATTCAGCTAGAACAAAAGGCAGAAAGCAAGAACTTTGAAACTACACATGCATGTAGTGAAAGCAG
TCCACATGGAGATGGCTACAGCTCAGGGGTTATTAAAGACATTTGGACAAAGATGGTAGGTAGGAGTTCT
GTGGCTGCAGTAGAGACAGAAAGAACTGGGGAGGAATTGTTTTCCACAGATGTAAATAACTACTGCTGCT
GTTTGGATACTGAGGCGAAGATGGAAGCCCTTCAAGAGCCTAGTAGGGCTGTGCAGAGATCAGAGTATCA
TCTCTGGGAGGGACAGAAGGAGAACATGGAGAAGAGAGCATTTGTCTCTAGTGAGCTGTCCAAGGTGGAT
GGTGGCGATTACACCACACCCTCTAAACCTTGGGATGTGGCCCAAGATAAGGAGAACACATTCATTCTTG
GAGGAGTTTATGGAGAGCTCAAAACATTCAACAGTGATGGGGAGTGGGCAGTTGTACCACCTGGTCACAC
AAAAGGGAGCTTGCTACAGTGTGCAGCCTCTGATGTGGTGACAATCGCCGGTACAGATGTCTTTATGACC
CCTGGAAACAGCTTTGCTCCTGGTCACAGGCAGCTCTGGAAGCCCTTTGTGTCATTTGAACAGAGTGACA
TGCCGAAGAGAGGGGAAAATGGAGTAAACAAGGGGTTTTCTTTTATCTTCCATGAAGACTTGCTAGGAGC
CTGCAGTAACTTTCAAGTTGAAGATCCTGGCCTTGAATACTCACTCTCTTCCTTTGACCTAAGCAACCCC
TTCTCACAAGTTCTCCACGTAGAGTGCTCATTTGAACCAGAAGGGATTGCATCTTTTAGCCCCAGTTTCA
AGCCGAAATCAATCCTCTGCTCTGATTCAGACAGTGAGGTTTTTCACCCCAGAATATGTGGTGTCGAAAG
GACACAGTACAGGGCTATTCGAATCTCTCCCCGGACTCACTTTCGCCCGATTTCTGCATCTGAACTATCC
CCTGGAGGAGGAAGTGAGTCAGAATTCGAATCTGAAAAAGATGAAGCGAGTGTTCCCATTCCTTCCCATG
TTGATGTATTTGAAGACCCACAAGCAGATCTCAAACCTTTAGAGGAAGATGCAGAGAAAGAAGGTCACTA
CTATGGAAAGTTAGAGCTTGAGTCTGGGAAGTTCCTGCCCAGGTTAAAAAAATCTGGAATGGAGAAGAGT
GCTCAGACATCACTGGATTCCCAGGAGGAGGCAACTGGGATTCTGCCGAAACAAAATCAGTGTTTGGAAT
GTAACTTTAACGAATCTCTAGAAATTAATTTAGAAAGCTCAGCAGCAAACTGTAAAATAATGACACAATG
TGAGGAAGAGATGAGTGAGTTTTGCAGTTGCAAAGCAGGTTGTCAGTTCCCTGCTTGTGAAGATAATCCA
GTTTCTTCAGGACAATTGGAAGAGTTTCCTGTACTGAACACTGATGTGCAAGAAGTAACAAGAAATCAAG
AAAAGCAGAGCTGGTGGGAAAAAGCTTTGTACTCTCCCCTTTTCCCCACGTCAGAGTGTGAAGAATGTTA
TACAAATGCCAAGGGAGAGAATGGTATAGAAGAATGTCCAGATGCTAAAGAGACACCCAGTCGTGAAGAG
CGTCTGTTAGATTTTAATAGGGTGTCTTCTGTTTATGAAGCAAGATGCACAGGAGAGAGAGGTTCTGAGA
CAAAACCAAATGGCCTCCACAGGAAGATGTGTTCCAGTGCCAGCTCTGACACGGGCGACACAGGCTCCGA
AGCCGGCGGAGAGTGGGTGGGCCCCAGCAGAGAGGAGCTCTTCTCCCGAACACATCTC

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG215792 representing NM_001081232
Red=Cloning site Green=Tags(s)

MRPVCTVVVDGLPSESTSSSYPGPVSVSDMSLLHALGPVQTWLGQELEKCGIDAMIYSRYILSLLLHDSY
DYDLQEQENDILSWEKGAYKKWGRSKKKCSDLTLEEMKKQAAVQCLRSASDESSGIETLVEELCCRLKDL
QSEQEEKIHKKLEGSPSPEEELSPTAKDQVEMYYEAFPPLSEKPVCLQEIMTVWNKSKPCSYSSSSSSST
VPPASTDTSSPKDCNSESEAVRERSSVASVPMHEKAQSRSRHEKESKLSSSTIEEKPAFYKRQIRHKPEG
KTRPRSWSSGSSEAGSSSSGNQGELKASMKYVKVRHKAREIRNRKGRNGQNRHSLKHCGKAERGVHAGSG
GSSSSSSNGSIRQLCKRGKRPAKETGRSDSGNTAVKDLYVDSRNNKEYKEEPLWYTEPIAEYFVPLSRKS
KLETTYRNREDTSTLTAEAVEDLSDSVRGLCISNSNIHRTYLAAGTFIDGHFVEMPAVINEDIDLAGTSL
CSLPEDNKYLDDIHLSELTHFYEVDIDQSMLDPGASETMQGESRILNMIRQKSKENTDFEAECCIVLDGM
ELQGERAIWTDSTSSVGAEGFFLQDLGNLAQFWECCSSSSGDADGESFGGDSPVRLSPILDSTMLSSHIL
AGNQEPFSNINEGSGINSCFSVFEVQCSNSVLPFSFETLNLGSEHADSSANMLGKTQSRLLIWTKNSAFE
ENEHCSNLSTRTCSPWSHSEEARSDNETLNIQFEESTQFTAEDINYVVPRVSSDFVDEELLDFLQDETCQ
QNSRTLGEIPTLVFKKRSKLESVCGIQLEQKAESKNFETTHACSESSPHGDGYSSGVIKDIWTKMVGRSS
VAAVETERTGEELFSTDVNNYCCCLDTEAKMEALQEPSRAVQRSEYHLWEGQKENMEKRAFVSSELSKVD
GGDYTTPSKPWDVAQDKENTFILGGVYGELKTFNSDGEWAVVPPGHTKGSLLQCAASDVVTIAGTDVFMT
PGNSFAPGHRQLWKPFVSFEQSDMPKRGENGVNKGFSFIFHEDLLGACSNFQVEDPGLEYSLSSFDLSNP
FSQVLHVECSFEPEGIASFSPSFKPKSILCSDSDSEVFHPRICGVERTQYRAIRISPRTHFRPISASELS
PGGGSESEFESEKDEASVPIPSHVDVFEDPQADLKPLEEDAEKEGHYYGKLELESGKFLPRLKKSGMEKS
AQTSLDSQEEATGILPKQNQCLECNFNESLEINLESSAANCKIMTQCEEEMSEFCSCKAGCQFPACEDNP
VSSGQLEEFPVLNTDVQEVTRNQEKQSWWEKALYSPLFPTSECEECYTNAKGENGIEECPDAKETPSREE
RLLDFNRVSSVYEARCTGERGSETKPNGLHRKMCSSASSDTGDTGSEAGGEWVGPSREELFSRTHL

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001081232
ORF Size 4188 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001081232.2
RefSeq Size 6720 bp
RefSeq ORF 4191 bp
Locus ID 320661
UniProt ID Q80U59
Cytogenetics 5 19.18 cM
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Documents
Resources
"NM_001081232" in other vectors (4)
SKU Description Size Price
MC224407 D5Ertd579e (untagged) - Mouse DNA segment, Chr 5, ERATO Doi 579, expressed (D5Ertd579e), (10ug) 10 ug
$1,314.00
MR215792 D5Ertd579e (Myc-DDK-tagged) - Mouse DNA segment, Chr 5, ERATO Doi 579, expressed (D5Ertd579e) 10 ug
$589.00 $1,194.00
MR215792L3 Lenti ORF clone of D5Ertd579e (Myc-DDK-tagged) - Mouse DNA segment, Chr 5, ERATO Doi 579, expressed (D5Ertd579e) 10 ug
$1,494.00
MR215792L4 Lenti ORF clone of D5Ertd579e (mGFP-tagged) - Mouse DNA segment, Chr 5, ERATO Doi 579, expressed (D5Ertd579e) 10 ug
$1,494.00

file-alt Citations

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