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Alg1 (NM_145362) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG215774
Alg1 (tGFP-tagged) - Mouse asparagine-linked glycosylation 1 homolog (yeast beta-14-mannosyltransferase) (Alg1), (10ug)
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ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $657.00
3 Weeks*
Bulk Requests & Clone Modifications

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Other products for "Alg1 or NM_145362"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Alg1
Synonyms HMAT1; HMT1
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG215774 representing NM_145362
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCAGCGTCCTGCGTGGCGCTGCTGGTTCTGGCGCTGCTGTTGCTGGTGCTGCTGCTGGGCTTGTGGA
AGCGTGGGCGGCAAACGGGCAGGGCCCGGCACATGGTCGTCGTGGTGCTGGGCGACGTGGGCCGCAGCCC
CCGCATGCAGTACCACGCGCTGTCATTGGCCCAGAGCGGCTTTTCGGTGACGCTGCTGGGGTTCTACAAC
TCCAAACCTCGAGATGAACTCTTGCAGAATGATAGGATTCGGATTGTGAAGTTGACAGATCTGCGGGGCC
TGGGAGCTGGGCCCCGAATTCTCCAGTATGGAGTCAAGGTTGTATTTCAGGCAGTGTACTTGCTGTGGAA
GATGATGCGCATGGACCCTGCAGCCTACATCTTCCTCCAGAACCCTCCAGGTCTGCCTGCTATTGCCGTC
TGCTGGTTTGTGGGCTGCATTTGTGGGAGCAAATTGGTCATCGACTGGCACAACTATGGCTACTCCATCA
TGGGCTTGGTCCATGGCCCCCGCCACCCCATTGTGCTGCTGGCCAAATGGTATGAGAAGTTCTTCGGGCG
CCTGTCCCACCTGAACCTGTGTGTGACCAATGCTATGCGGGAGGACCTGGCAGAGAACTGGTGTGTCAGG
GCTGTGACGCTCTACGACAAGCCAGCATCTTTCTTTAAGGAGACACCCCTGGACCTGCAGCATGAACTCT
TTATGAAGCTGAGCCACACGTACTCTCCTTTCCAGAGCTGCTCAGATCCCTCACATCCTGACACAGAGAG
GTCGGCCTTCACTGAGAGGGATTGTCAGAGCGGGGTTGTGAGGCGTCTGCATGGGCGGCCAGCACTGCTC
GTGAGCAGCACAAGCTGGACAGAGGACGAGGACTTCTCCATCCTGCTTCGAGCATTAGAAAAGTTTGAGC
AACAAGCACTCACTGGAGACAGCCTCCCTTCCCTCGTCTGTGTGATAACAGGGAAGGGACCGCTCAGAGA
GCACTACCGCCACCTCATCAGCCAGAAGCACCTTCAGCACGTCCGGTTCTGCACCCCATGGCTGGAAGCC
GAGGACTACCCACTGCTTCTAGGGTCAGCGGATCTGGGTGTCTGTCTACACATGTCCTCCAGTGGTCTGG
ACCTGCCCATGAAGGTGGTGGACATGTTTGGGTGCCACTTGCCTGTGTGTGCCGTGAACTTCAAGTGTCT
GCACGAGCTAGTAAGACATGGAGAGAATGGTCTGGTCTTCAAGGATGCTGAGGAGCTGGCAGCTCAGCTG
CAGATGCTTTTCTCAAAGTTCCCAGACCCTGCTGGAAAGCTAAGCCAGTTCCGGAAGAAACTGCAGGAGT
CGGGGCAGCAACGCTGGGATGAGAGCTGGCAGCACACTGTGCTCCCTCTGCTCGCTCACTCACAGATGAC
CCCCAGGCCCCATCCACCCTGTGGCCACCCTTCCTGCCGGGGCTTT

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG215774 representing NM_145362
Red=Cloning site Green=Tags(s)

MAASCVALLVLALLLLVLLLGLWKRGRQTGRARHMVVVVLGDVGRSPRMQYHALSLAQSGFSVTLLGFYN
SKPRDELLQNDRIRIVKLTDLRGLGAGPRILQYGVKVVFQAVYLLWKMMRMDPAAYIFLQNPPGLPAIAV
CWFVGCICGSKLVIDWHNYGYSIMGLVHGPRHPIVLLAKWYEKFFGRLSHLNLCVTNAMREDLAENWCVR
AVTLYDKPASFFKETPLDLQHELFMKLSHTYSPFQSCSDPSHPDTERSAFTERDCQSGVVRRLHGRPALL
VSSTSWTEDEDFSILLRALEKFEQQALTGDSLPSLVCVITGKGPLREHYRHLISQKHLQHVRFCTPWLEA
EDYPLLLGSADLGVCLHMSSSGLDLPMKVVDMFGCHLPVCAVNFKCLHELVRHGENGLVFKDAEELAAQL
QMLFSKFPDPAGKLSQFRKKLQESGQQRWDESWQHTVLPLLAHSQMTPRPHPPCGHPSCRGF

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_145362
ORF Size 1446 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_145362.2, NP_663337.2
RefSeq Size 1772 bp
RefSeq ORF 1449 bp
Locus ID 208211
UniProt ID Q921Q3
Cytogenetics 16 A1
Summary Participates in the formation of the lipid-linked precursor oligosaccharide for N-glycosylation. Involved in assembling the dolichol-pyrophosphate-GlcNAc(2)-Man(5) intermediate on the cytoplasmic surface of the ER (By similarity).UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_145362" in other vectors (4)
SKU Description Size Price
MC216706 Alg1 (untagged) - Mouse asparagine-linked glycosylation 1 homolog (yeast, beta-1,4-mannosyltransferase) (Alg1), (10ug) 10 ug
$503.00
MR215774 Alg1 (Myc-DDK-tagged) - Mouse asparagine-linked glycosylation 1 homolog (yeast, beta-1,4-mannosyltransferase) (Alg1) 10 ug
$289.00 $457.00
MR215774L3 Lenti ORF clone of Alg1 (Myc-DDK-tagged) - Mouse asparagine-linked glycosylation 1 homolog (yeast, beta-1,4-mannosyltransferase) (Alg1) 10 ug
$757.00
MR215774L4 Lenti ORF clone of Alg1 (mGFP-tagged) - Mouse asparagine-linked glycosylation 1 homolog (yeast, beta-1,4-mannosyltransferase) (Alg1) 10 ug
$757.00

file-alt Citations

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