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Ptprn2 (NM_011215) Mouse Tagged ORF Clone

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SKU
MG215745
Ptprn2 (tGFP-tagged) - Mouse protein tyrosine phosphatase receptor type N polypeptide 2 (Ptprn2), (10ug)
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ORF Plasmid:
tGFP DDK
  $1,072.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Ptprn2
Synonyms 4930425H11Rik; IA2beta; mKIAA0387; phogrin; Phol; PTP-NP
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG215745 representing NM_011215
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGGCCGCCGCTCCCGCTTTTGCTGCTGCTGCTGCTACCGCCGCCGCTGCCTCGCGCTCTGCCCGCCC
CCGCGTCTGCCCGCGGCCGGCAGCTCCCGGGGCGCCTGGGATGCTTGTTTGAGGATGGCTTGTGTGGATC
ACTGGAGACCTGTGTGAACGATGGTGTGTTTGGAAGATGTCAAAAGGTTCCGGTGATGGACACTTACCGA
TATGAGGTACCACCAGGAGCCCTGCTGCACCTGAAGGTCACCTTACAGAAGCTCTCCCGTACAGGTTTCA
CGTGGCAAGATGACTATACCCAGCGTGTGATCGCCCAGGAGCTTGCAAACCTCCCCAAGGCCTACCTATG
GCATGGGGAAACGTCCGGCCCACCCAGGTCCTTACAACAGAATGCTGACAATGAAAAATGGTTCAGTCTG
GAGAGGGAGGTGGCCCTGGCCAAGACCCTTCGGCGCTATCTGCCCTACCTGGAGCTTCTGTCCCAGACCC
CAACAGCAAATGCACACTCTAGGATAGACCATGAGACTCGTCCAGCCAAGGGTGAAGACTCTTCCCCTGA
GAACATCCTGACCTACGTGGCCCACACATCAGCACTGACCTATCCTCCTGCAACCCGGGCCAAGTATCCT
GATAACCTTCTGCGGCCCTTTAGCCGGCTCCAGCCAGATGAGCTCAGCCCCAAGGTAGACGGTGACATAG
ACAAACAGAAACTGATTGCAGCACTGGGCGCCTACACTGCTCAGAGGCTTCCTGGAGAAAATGACCCAGA
GCCACGGTACCTTGTACATGGTTCCTCGAGAGCACCAAGGCCATTCTCAGCAACTGCTTTGTCTCAGAGA
TGGCCTCCACCTCCTGGAGACGCCAAAGACTCCCCGAGTATGGATGATGACACACTCCTGCAGAGTCTCC
TGAAGGATTTGCAGCAGAACTCTGAAGTGGACCGCCTGGGCCCCCTGAAGGAGGAGAAAGCAGACTCAGT
TGCTGGAGCCATACAAAGTGATCCTGCAGAGGGAAGCCAAGAAAGCCACGGGAGAGGGGCTGAAGGACAG
CCAAGAGAGCAGACAGATGCCCCAGAGACAATGCTTCAAGATCACAGACTATCAGAGGTGGATGACCCAG
TGTACAAGGAGGTCAACCGTCTGAGCTTCCAGCTTGGGGACCTCTTGAAGGACTATGGGTCTCCTCTCTT
ACCTGAAGGTCCCCTTCTAGAAAAATCCTCCAGAGAAGAGATTAAGAAGTCAGAGCAGCCAGAGGAGGTC
TTGTCTTCAGAAGAGGAGACTGCTGGGGTGGAGCATGTGAGGAGCCGGACTTACTCCAAAGACCTATTTG
AAAGGAAACCAAACTCAGAGCCCCAGCCCAGGAGGCTTGAGGATCAGTTCCAAAACCGAGCTCCAGAGTT
GTGGGAGGATGAAGAAAGCCTCAAATTGGCAGCACAGGGACCCCCTAGTGGAGGCCTACAGCTGGAAGTG
CAGCCTTCTGAGGAACAGCAGGGATACATCCTCACAGGAAACAACCCTCTAAGTCCAGAGAAGGGGAAGC
AGCTGATGGACCAAGTTGCCCACATCCTCCGGGTACCTTCCAGCTTCTTTGCAGATATCAAAGTTTTGGG
ACCAGCAGTGACCTTCAAAGTAAGTGCCAACATCCAAAACATGACAACTGCCGATGTCATCAAGGCTGCA
GCTGACAACAAAGACCAGCTGGAGAAGGCAACTGGACTGACAATCCTTCAAAGTGGAATCAGGCCGAAGG
GAAAGCTCAAACTCCTGCCGCATCAGGAAGAGCAAGAGGACTCTACCAAGTTCATTTTGCTCACCTTCCT
CTCCATTGCCTGCATCCTGGGGGTTCTCCTGGCTTCCAGCCTTGCCTACTGCCTCCGCCACAACTCACAC
TACAAGCTGAAGGACAAGTTGTCTGGACTAGGCGCTGACCCCAGTGCAGATGCCACTGAAGCCTACCAGG
AGCTATGCCGCCAGCGTATGGCTGTTCGTCCACAGGACCGCTCTGAGGGACCACATACATCACGCATCAA
CAGCGTCTCATCCCAGTTCAGCGATGGGCCGATGCCTAGTCCTTCGGCTCGGAGCAGCACTTCATCCTGG
TCTGAGGAGCCTGTCCAGTCCAACATGGACATCTCTACTGGCCACATGATCCTGGCCTACATGGAAGACC
ATCTGAAGAACAAGAACCGCCTGGAGAAGGAGTGGGAAGCACTGTGCGCCTACCAAGCAGAGCCCAACAG
CTCACTTGTGGCCCAGAGAGAGGAGAATGCACCCAAGAACCGTTCCCTGGCTGTGCTGACCTATGACCAC
TCCAGGATCCTGTTGAAGTCTCAAAACAGCCATGGCAGTTCCGACTACATCAATGCCAGCCCCATTATGG
ACCATGACCCACGAAACCCCGCATACATTGCCACCCAAGGCCCACTTCCCGCCACGGTGGCCGACTTCTG
GCAGATGGTGTGGGAAAGCGGCTGTGCAGTCATTGTCATGCTGACACCCCTCTCCGAGAACGGCGTCCGG
CAGTGCCATCACTACTGGCCCGATGAAGGCTCCAACCTCTACCATGTCTACGAGGTCAATCTAGTCTCTG
AGCACATATGGTGCCAGGATTTCCTGGTGAGAAGCTTTTACCTGAAGAACCTGCAGACCAACGAGACTCG
CACGGTGACCCAGTTCCACTTCCTGAGTTGGTATGACCAGGGAGTCCCTTCCTCCACGAGGTCACTCCTG
GATTTCCGCAGAAAAGTGAACAAATGCTACCGAGGCCGCTCTTGTCCGATCATTGTCCATTGCAGTGACG
GCGCCGGCAGGAGTGGAACCTACGTCCTGATTGACATGGTTCTCAATAAGATGGCCAAAGGTGCTAAAGA
GATTGATATCGCAGCGACCCTGGAGCACTTGAGGGACCAGAGACCAGGCATGGTCCAGACAAAGGAGCAG
TTTGAGTTTGCGCTGACAGCTGTGGCTGAGGAGGTGAATGCCATCCTGAAGGCCCTTCCCCAG

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG215745 representing NM_011215
Red=Cloning site Green=Tags(s)

MGPPLPLLLLLLLPPPLPRALPAPASARGRQLPGRLGCLFEDGLCGSLETCVNDGVFGRCQKVPVMDTYR
YEVPPGALLHLKVTLQKLSRTGFTWQDDYTQRVIAQELANLPKAYLWHGETSGPPRSLQQNADNEKWFSL
EREVALAKTLRRYLPYLELLSQTPTANAHSRIDHETRPAKGEDSSPENILTYVAHTSALTYPPATRAKYP
DNLLRPFSRLQPDELSPKVDGDIDKQKLIAALGAYTAQRLPGENDPEPRYLVHGSSRAPRPFSATALSQR
WPPPPGDAKDSPSMDDDTLLQSLLKDLQQNSEVDRLGPLKEEKADSVAGAIQSDPAEGSQESHGRGAEGQ
PREQTDAPETMLQDHRLSEVDDPVYKEVNRLSFQLGDLLKDYGSPLLPEGPLLEKSSREEIKKSEQPEEV
LSSEEETAGVEHVRSRTYSKDLFERKPNSEPQPRRLEDQFQNRAPELWEDEESLKLAAQGPPSGGLQLEV
QPSEEQQGYILTGNNPLSPEKGKQLMDQVAHILRVPSSFFADIKVLGPAVTFKVSANIQNMTTADVIKAA
ADNKDQLEKATGLTILQSGIRPKGKLKLLPHQEEQEDSTKFILLTFLSIACILGVLLASSLAYCLRHNSH
YKLKDKLSGLGADPSADATEAYQELCRQRMAVRPQDRSEGPHTSRINSVSSQFSDGPMPSPSARSSTSSW
SEEPVQSNMDISTGHMILAYMEDHLKNKNRLEKEWEALCAYQAEPNSSLVAQREENAPKNRSLAVLTYDH
SRILLKSQNSHGSSDYINASPIMDHDPRNPAYIATQGPLPATVADFWQMVWESGCAVIVMLTPLSENGVR
QCHHYWPDEGSNLYHVYEVNLVSEHIWCQDFLVRSFYLKNLQTNETRTVTQFHFLSWYDQGVPSSTRSLL
DFRRKVNKCYRGRSCPIIVHCSDGAGRSGTYVLIDMVLNKMAKGAKEIDIAATLEHLRDQRPGMVQTKEQ
FEFALTAVAEEVNAILKALPQ

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_011215
ORF Size 3003 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_011215.2, NP_035345.2
RefSeq Size 4688 bp
RefSeq ORF 3006 bp
Locus ID 19276
UniProt ID P80560
Cytogenetics 12 62.65 cM
Summary Plays a role in vesicle-mediated secretory processes (PubMed:21732083). Required for normal accumulation of secretory vesicles in hippocampus, pituitary and pancreatic islets. Required for the accumulation of normal levels of insulin-containing vesicles and preventing their degradation (PubMed:21732083). Plays a role in insulin secretion in response to glucose stimuli (PubMed:15220191, PubMed:16418280, PubMed:21732083). Required for normal accumulation of the neurotransmitters norepinephrine, dopamine and serotonin in the brain. In females, but not in males, required for normal accumulation and secretion of pituitary hormones, such as luteinizing hormone (LH) and follicle-stimulating hormone (FSH) (PubMed:16269463). Required to maintain normal levels of renin expression and renin release (PubMed:19019914). May regulate catalytic active protein-tyrosine phosphatases such as PTPRA through dimerization (PubMed:12364328). Has phosphatidylinositol phosphatase activity; the PIPase activity is involved in its ability to regulate insulin secretion. Can dephosphorylate phosphatidylinositol 4,5-biphosphate (PI(4,5)P2), phosphatidylinositol 5-phosphate and phosphatidylinositol 3-phosphate (By similarity). Regulates PI(4,5)P2 level in the plasma membrane and localization of cofilin at the plasma membrane and thus is indirectly involved in regulation of actin dynamics related to cell migration and metastasis; upon hydrolyzation of PI(4,5)P2 cofilin is released from the plasma membrane and acts in the cytoplasm in severing F-actin filaments (By similarity).UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_011215" in other vectors (4)
SKU Description Size Price
MC223149 Ptprn2 (untagged) - Mouse protein tyrosine phosphatase, receptor type, N polypeptide 2 (Ptprn2), (10ug) 10 ug
$959.00
MR215745 Ptprn2 (Myc-DDK-tagged) - Mouse protein tyrosine phosphatase, receptor type, N polypeptide 2 (Ptprn2) 10 ug
$589.00 $872.00
MR215745L3 Lenti ORF clone of Ptprn2 (Myc-DDK-tagged) - Mouse protein tyrosine phosphatase, receptor type, N polypeptide 2 (Ptprn2) 10 ug
$1,172.00
MR215745L4 Lenti ORF clone of Ptprn2 (mGFP-tagged) - Mouse protein tyrosine phosphatase, receptor type, N polypeptide 2 (Ptprn2) 10 ug
$1,172.00

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