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Ndufa3 (NM_025348) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG215702
Ndufa3 (tGFP-tagged) - Mouse NADH dehydrogenase (ubiquinone) 1 alpha subcomplex 3 (Ndufa3), (10ug)
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ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $350.00
3 Weeks*
Bulk Requests & Clone Modifications

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Other products for "Ndufa3 or NM_025348"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Ndufa3
Synonyms 1010001M12Rik; 1700022J01Rik
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG215702 representing NM_025348
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCCGGGAGAATCTCTGCCTTCCTCAAGAATGCCTGGGCGAAGGAGCCGGTGCTGGTGGTGTCCTTCT
CTGTCTGGGGCCTCGCTATAATTATGCCCATGATTAGCCCCTACACCAAGTATGCTAGCATGATCAACAA
GGCCACACCCTACAACTACCCAGTGCCTGTGAGAGATGACGGGAACATGCCTGATGTGCCCAGCCACCCT
CAGGATCCTCTGGGTCCAAGCTTGGACTGGCTGAAGAACCTG

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG215702 representing NM_025348
Red=Cloning site Green=Tags(s)

MAGRISAFLKNAWAKEPVLVVSFSVWGLAIIMPMISPYTKYASMINKATPYNYPVPVRDDGNMPDVPSHP
QDPLGPSLDWLKNL

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_025348
ORF Size 252 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_025348.3
RefSeq Size 349 bp
RefSeq ORF 255 bp
Locus ID 66091
UniProt ID Q9CQ91
Cytogenetics 7 A1
Summary Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_025348" in other vectors (4)
SKU Description Size Price
MC210268 Ndufa3 (untagged) - Mouse NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 3 (Ndufa3), (10ug) 10 ug
$165.00
MR215702 Ndufa3 (Myc-DDK-tagged) - Mouse NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 3 (Ndufa3) 10 ug
$289.00
MR215702L3 Lenti ORF clone of Ndufa3 (Myc-DDK-tagged) - Mouse NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 3 (Ndufa3) 10 ug
$450.00
MR215702L4 Lenti ORF clone of Ndufa3 (mGFP-tagged) - Mouse NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 3 (Ndufa3) 10 ug
$450.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.