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Zyg11b (NM_001033634) Mouse Tagged ORF Clone

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SKU
MG215674
Zyg11b (tGFP-tagged) - Mouse zyg-ll homolog B (C. elegans) (Zyg11b), (10ug)
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ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $881.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Zyg11b
Synonyms 1110046I03Rik; 2810482G21Rik; D4Mgi23; Gm431; mKIAA1730
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG215674 representing NM_001033634
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCCGGAGGACCAGGCCCACGCAGCCATGGAGGAAGCCTCTCCCTATTCCTTGCTTGACATCTGCTTGA
GTTTCCTGACCACCAACCTCGAGAAGTTCTGCTCAGCACGCCAAGATGGAACCCTGTGTCTGCAGGAGCC
TGGGGTGTTCCCACAGGAGGTGGCTGACCGGCTTCTTCAGACTATAGCATTCCACGGTCTCCTGAATGAT
GGAACTGTGGGAATTTTCAGAGGTAACCAGATGCGCCTAAAGCGAGCTTGCATTCGCAAAGCAAAGATTT
CCGCTGTTGCTTTCCGGAAAGCCTTTTGCCACCACAAGCTAGTAGAACTTGATGCCACGGGTGTGAATGC
TGACATCACTATCACAGACATCATAAGTGGCCTTGGCAGTAACAAGTGGATCCAGCAAAATCTTCAGTGC
TTAGTACTAAACTCATTAACTCTGTCCCTCGAGGATCCATATGAGCGGTGCTTCAGCCGGCTCTCTGGTC
TTCGAGCTCTGAGCATCACTAATGTGCTCTTTTACAATGAAGATCTGGCTGAAGTTGCTTCGCTGCCTAG
ACTGGAGAGCCTGGATATTTCTAACACCTCAATCACAGATATCACTGCCCTGCTGGCCTGCAAAGACAGA
CTCAAGTCTCTCACCATGCACCATCTGAAATGTTTAAAAATGACTACTACCCAGATCCTGGATGTCGTTC
GGGAGCTAAAACATCTGAACCATCTTGACATCTCAGATGATAAACAGTTCACGTCAGACATAGCCCTTCG
ATTGTTAGAGCAAAAGGACATCTTGCCCAATCTTGTGTCCCTGGATGTTTCAGGGAGAAAGCATGTGACA
GACAAAGCTGTCGAAGCCTTTATACAGCAGCGGCCTAGCATGCAGTTCGTAGGGTTGCTGGCTACAGATG
CTGGATACTCTGAATTCCTTATGGGCAAAGGACACTTGAAGGTCTCTGGAGAAGCCAACGAAACCCAGAT
TGCAGAGGCGCTGAGGCGGTACAGCGAGCGGGCGTTTTTCGTTAGGGAGGCTTTGTTTCATCTCTTTAGC
CTGACTCATGTGATGGAGAAAACGAAGCCAGACATTTTAAAGCTTGTGGTTACTGGGATGAGAAACCACC
CAATGAATTTGCCCGTGCAGCTGGCTGCAAGTGCCTGTGTGTTTAACTTAACTAAGCAGGACCTTGCTTT
AGGCATGCCTGTCCGGCTCCTCGCTGATGTGACCCATTTGCTGCTCAAGGCCATGGAACATTTTCCTAAT
CACCAACAGTTACAGAAGAATTGCCTCCTCTCACTTTGCAGTGACCGGATTCTTCAAGATGTTCCATTTA
ATAGGTTTGAAGCAGCCAAGCTTGTCATGCAGTGGCTTTGCAACCACGAAGATCAGAACATGCAACGCAT
GGCCGTGGCTATCATTTCCATCCTGGCCGCCAAGCTCTCTACAGAACAAACTGCACAGCTTGGTGCTGAG
CTCTTCATTGTCAGGCAACTTCTTCAAATAGTGAAGCAGAAAACTAATCAGAATTCCGTGGACACTACGT
TGAAGTTTACTTTGAGTGCACTTTGGAACCTCACAGATGAGTCCCCAACCACGTGCAGACACTTCATTGA
AAACCAAGGGCTGGAGCTCTTCATGAGGGTTCTAGAGTCTTTCCCAACTGAGTCATCCATTCAACAAAAG
GTTCTAGGACTTTTGAACAACATAGCTGAAGTACAAGAGCTGCATTCTGAACTAATGTGGAAGGATTTTA
TAGACCACATCAGCAGTCTCCTACACAGTGTTGAAGTAGAAGTCAGTTACTTCGCAGCTGGAATTATCGC
CCATTTAATATCCAGAGGTGAACAAGCATGGACCTTGAGCCGAAGCCAGAGGAATTCTCTTCTTGATGAT
TTGCATTCAGCTATTTTGAAATGGCCAACTCCAGAGTGTGAGATGGTAGCATACAGGTCCTTTAATCCAT
TCTTTCCACTACTTGGCTGTTTTACGACTCCTGGAGTCCAGCTGTGGGCAGTTTGGGCCATGCAGCACGT
CTGCAGCAAGAATCCTTCAAGGTATTGCAGCATGCTGATTGAAGAAGGAGGACTGCAGCATTTGTATAAC
ATCAAAGAACACGAGCAGACAGATCCCTATGTCCAGCAGATTGCTGTGGCCATTCTGGACAGTTTAGAGA
AACACATTGTGCGCCATGGAAGGCCCCCTCCCTGTAAAAAGCAGCCCCAGGCCCGGCTGAAT

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG215674 representing NM_001033634
Red=Cloning site Green=Tags(s)

MPEDQAHAAMEEASPYSLLDICLSFLTTNLEKFCSARQDGTLCLQEPGVFPQEVADRLLQTIAFHGLLND
GTVGIFRGNQMRLKRACIRKAKISAVAFRKAFCHHKLVELDATGVNADITITDIISGLGSNKWIQQNLQC
LVLNSLTLSLEDPYERCFSRLSGLRALSITNVLFYNEDLAEVASLPRLESLDISNTSITDITALLACKDR
LKSLTMHHLKCLKMTTTQILDVVRELKHLNHLDISDDKQFTSDIALRLLEQKDILPNLVSLDVSGRKHVT
DKAVEAFIQQRPSMQFVGLLATDAGYSEFLMGKGHLKVSGEANETQIAEALRRYSERAFFVREALFHLFS
LTHVMEKTKPDILKLVVTGMRNHPMNLPVQLAASACVFNLTKQDLALGMPVRLLADVTHLLLKAMEHFPN
HQQLQKNCLLSLCSDRILQDVPFNRFEAAKLVMQWLCNHEDQNMQRMAVAIISILAAKLSTEQTAQLGAE
LFIVRQLLQIVKQKTNQNSVDTTLKFTLSALWNLTDESPTTCRHFIENQGLELFMRVLESFPTESSIQQK
VLGLLNNIAEVQELHSELMWKDFIDHISSLLHSVEVEVSYFAAGIIAHLISRGEQAWTLSRSQRNSLLDD
LHSAILKWPTPECEMVAYRSFNPFFPLLGCFTTPGVQLWAVWAMQHVCSKNPSRYCSMLIEEGGLQHLYN
IKEHEQTDPYVQQIAVAILDSLEKHIVRHGRPPPCKKQPQARLN

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001033634
ORF Size 2232 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001033634.3, NP_001028806.2
RefSeq Size 10654 bp
RefSeq ORF 2235 bp
Locus ID 414872
UniProt ID Q3UFS0
Cytogenetics 4 C7
Summary Probably acts as target recruitment subunit in the E3 ubiquitin ligase complex ZYG11B-CUL2-Elongin BC.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_001033634" in other vectors (4)
SKU Description Size Price
MC221266 Zyg11b (untagged) - Mouse zyg-ll homolog B (C. elegans) (Zyg11b), (10ug) 10 ug
$750.00
MR215674 Zyg11b (Myc-DDK-tagged) - Mouse zyg-ll homolog B (C. elegans) (Zyg11b) 10 ug
$589.00 $681.00
MR215674L3 Lenti ORF clone of Zyg11b (Myc-DDK-tagged) - Mouse zyg-ll homolog B (C. elegans) (Zyg11b) 10 ug
$981.00
MR215674L4 Lenti ORF clone of Zyg11b (mGFP-tagged) - Mouse zyg-ll homolog B (C. elegans) (Zyg11b) 10 ug
$981.00

file-alt Citations

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