Sync (NM_023485) Mouse Tagged ORF Clone

  • TrueORF®
SKU
MG215638
Sync (tGFP-tagged) - Mouse syncoilin (Sync), (10ug)
  $657.00
3 Weeks*
Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Sync
Synonyms 1110057H03Rik; SNIP4
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG215638 representing NM_023485
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCCAGCCCGGAACCCCTGCGCGGCGGGGACGGCGCCCGGGCCTCGAGGGAACCACACACCGAGGAGA
GTTTTCCTCTTCAGGAGTCAGAATCCCCGAAGGAGGCCAAGACCTTTAACCCAGAGGCCACTCTGTCTTT
GGAGGGGACTGTGAACCTAGAGGACATTCTCTATCTGGGGGCATCGGGGGACTTTGAAGAGAGTTTTTAT
GAGGAAGAATATGAGAAGCCAGCACAGACACTGTTTATCGACGAATCGAGGCAGCCAGACGAGGCGCTGG
GCCTAGAAGAGCCCGTGCGCCCAGAGGAGATGCTGTCTGTGGAGGAATCCGTGACGCCAGATGAGGTGCA
AATTTCAGAGCAGCCTGTGGAGCCAGCTAAGAGCCCAACTGCTTGTGAGGGAGAAATGGTGGCCACGGAG
GGAAGCCTGCCTGCTCAACCGATTCCCAACACCGAGGAGGATCCCCTCAGCGTGGAGGACCTGGAGCGGC
TGGAAGCTCGTTTCCAGCAGTGTGTACAAGCCGTGTCCCAGCTGGAAGAGGAGAGGGACCAGCTAATCCA
CGAGCTCGTGCTACTCCGGGAGCCAGCCCTGCAGGAGGTGCAGCAAGTCCACCAGGACATCCTGGCTGCC
TACAAGCTCCACGCACAGGCAGAGCTGGAGAGGGACGGGCTCAGGGAGGAGATCCGGACGGTGAAGCAGA
AGCTGTTCAAGGTGACCAAGGAATGCGTGGCTTACCAATACCAGCTGGAGTGCCGTCAGCAGGATGTGGC
TCAGTTTGCCGACTGCAGGGAAGCGCTGACCACTCGGGCAGCCCAGCTCTCGGAAGAACTGACCCAGCTC
CGAGATGCCTGTCAGAAGCAGAAGGAGCAGTTACAGCAACAACTAGAAGCACCCCCGACCCAGAGCGATG
GGCACTTTCTCCAGGAGAGCCGACGGCTGTCGACACAGTTTGAAAATCTCATGGCCGAGAGCCGCCAGGG
CCTGGAGGAGGAGTATGAGCCTCAGCTGCTCCGGCTCCTGGAAAGGAAAGAGGCCGGGACCAAAGCTCTA
CAGGACACCCAGGCGGAGATCCAGGAGATGAGGGAGGCTCTGCGACCCCTGGAAGCAGAGGCCCGGCAGC
TCCAACTTCAGAACAGGAACCTGGAGGACCAGATCACGCTCGTGAGGCAAAAACGCGATGAAGAGGTTCA
ACAGTACAGGGAGCAGCTGGAGGAGATGGAGGAGAGACAGAGGCAGTTGCGCAGTGGGGTGCAAGTTCAG
CAACAGAAGAACAAGGAGATGGAGCGGCTGAGGACGAGCCTTGCTGAAGAGCTTTCCACATATAAGGCTA
TGCTACCCAAGAGTCTGGAACAGGCTGATGCACCCACTTCTCAGGCAGGTGGAGTCGAGGCCCAATCCCC
AGGGACTGTT


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG215638 representing NM_023485
Red=Cloning site Green=Tags(s)

MASPEPLRGGDGARASREPHTEESFPLQESESPKEAKTFNPEATLSLEGTVNLEDILYLGASGDFEESFY
EEEYEKPAQTLFIDESRQPDEALGLEEPVRPEEMLSVEESVTPDEVQISEQPVEPAKSPTACEGEMVATE
GSLPAQPIPNTEEDPLSVEDLERLEARFQQCVQAVSQLEEERDQLIHELVLLREPALQEVQQVHQDILAA
YKLHAQAELERDGLREEIRTVKQKLFKVTKECVAYQYQLECRQQDVAQFADCREALTTRAAQLSEELTQL
RDACQKQKEQLQQQLEAPPTQSDGHFLQESRRLSTQFENLMAESRQGLEEEYEPQLLRLLERKEAGTKAL
QDTQAEIQEMREALRPLEAEARQLQLQNRNLEDQITLVRQKRDEEVQQYREQLEEMEERQRQLRSGVQVQ
QQKNKEMERLRTSLAEELSTYKAMLPKSLEQADAPTSQAGGVEAQSPGTV

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_023485
ORF Size 1410 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_023485.3, NP_075974.3
RefSeq Size 2038 bp
RefSeq ORF 1413 bp
Locus ID 68828
UniProt ID Q9EPM5
Cytogenetics 4 63.26 cM
Summary Atypical type III intermediate filament (IF) protein that may play a supportive role in the efficient coupling of mechanical stress between the myofibril and fiber exterior. May facilitate lateral force transmission during skeletal muscle contraction. Does not form homofilaments nor heterofilaments with other IF proteins.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_023485" in other vectors (4)
SKU Description Size Price
MC216510 Sync (untagged) - Mouse syncoilin (Sync), (10ug) 10 ug
$503.00
MR215638 Sync (Myc-DDK-tagged) - Mouse syncoilin (Sync) 10 ug
$289.00 $457.00
MR215638L3 Lenti ORF clone of Sync (Myc-DDK-tagged) - Mouse syncoilin (Sync) 10 ug
$757.00
MR215638L4 Lenti ORF clone of Sync (mGFP-tagged) - Mouse syncoilin (Sync) 10 ug
$757.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.