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Mill2 (NM_153760) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG215604
Mill2 (tGFP-tagged) - Mouse MHC I like leukocyte 2 (Mill2) transcript variant 2, (10ug)
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ORF Plasmid:
tGFP DDK
  $703.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Mill2
Synonyms Micb
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG215604 representing NM_153760
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAAAGCCAGTTCTGGAAAGCCCAGAGAATTCAGACCAGCAGTCCTACTGTTGATCTTAGGGCTGCTCC
TTAGAGACTCACGGGGATCCAGCATCCAAGGAACCCACACTCTGCGCTATAATGTCAGAGCCCATTCTCT
GGAGGGGTCTGAGAAGACCCAGCTCCTGGTCCTAATATACGTAGATGAAGAGCTCTTCCTGAAATACAAT
GGAGACAGCAGGGAGACAGAGCCCTTGGGATGCTGGATTAAGGGCCATGGGGGAAATGAGACCTGTGCAA
GAGAGACTAACAATCTGTTGAAGGTGGAAGAGAAACTCAGGGGGATGATGGCTGAGGTTATAAACCAGAA
GAGTCAGGAGGAAGGCCTTCACACTCTCCAGGCAACTTTGGGCTGTGAGCTTCTGAGTAATGGGAGCACT
AGAGGCTTCTGGCACCTGGGCTATGATGGGCAGAACTTCCTCACCTTTGACCAGAAGACTCTCACGTGGA
CAGTGGATGGACCCTCCACCCAGCAGAACAAGATGTTCTGGAAGACACATGCACCCAGAGCTGATCTAGT
TAAGACATTCTTGGATGATATATGTCCTGCTCACCTCCAGAGATACCTCGCTTCCTTGAGAAACGGTCTG
CAGGATACAGGACCCCCAATGGTAACTGTGACCTGCAGGAACTATCCAGTAGGTAGGGTCACACTGACAT
GCCGGGCTTTTAATCTGTATACCCGTGAGGCCACCCTGGTCTGGCTTCAGGATGGGAAGCCAGTACAGCA
GAAAACCTTCAGATCTGAGACCATCCTGCCCAGCGGGGATGGGACCTACCAGGCCCGGGTGTCCATTCGG
GTTCTTCCTGGACAGGAACCACAGTTCAGCTGCAACCTGAGGCATGGCAACCATAGCATCATGCAGACAG
CTGTCTCTGGGCATGCTGCTGAGGACAGTCAGGATGTTGCTAGCTCTGCTACAGCTTCAGCTGGATCTGC
ACTCCCTGTTGTATTGGCGGTGGCACTGGCCAGAGCCAAC

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG215604 representing NM_153760
Red=Cloning site Green=Tags(s)

MKASSGKPREFRPAVLLLILGLLLRDSRGSSIQGTHTLRYNVRAHSLEGSEKTQLLVLIYVDEELFLKYN
GDSRETEPLGCWIKGHGGNETCARETNNLLKVEEKLRGMMAEVINQKSQEEGLHTLQATLGCELLSNGST
RGFWHLGYDGQNFLTFDQKTLTWTVDGPSTQQNKMFWKTHAPRADLVKTFLDDICPAHLQRYLASLRNGL
QDTGPPMVTVTCRNYPVGRVTLTCRAFNLYTREATLVWLQDGKPVQQKTFRSETILPSGDGTYQARVSIR
VLPGQEPQFSCNLRHGNHSIMQTAVSGHAAEDSQDVASSATASAGSALPVVLAVALARAN

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_153760
ORF Size 1020 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_153760.2, NP_715641.1
RefSeq Size 1075 bp
RefSeq ORF 1023 bp
Locus ID 243864
UniProt ID Q8HWE6
Cytogenetics 7 9.35 cM
Summary Binds to heparan sulfate proteoglycans on the surface of fibroblast (NIH-3T3) cells.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_153760" in other vectors (4)
SKU Description Size Price
MC213176 Mill2 (untagged) - Mouse MHC I like leukocyte 2 (Mill2), transcript variant 2, (10ug) 10 ug
$503.00
MR215604 Mill2 (Myc-DDK-tagged) - Mouse MHC I like leukocyte 2 (Mill2), transcript variant 2 10 ug
$289.00 $503.00
MR215604L3 Lenti ORF clone of Mill2 (Myc-DDK-tagged) - Mouse MHC I like leukocyte 2 (Mill2), transcript variant 2 10 ug
$803.00
MR215604L4 Lenti ORF clone of Mill2 (mGFP-tagged) - Mouse MHC I like leukocyte 2 (Mill2), transcript variant 2 10 ug
$803.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.