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Retreg2 (NM_170755) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG215599
Fam134a (tGFP-tagged) - Mouse family with sequence similarity 134 member A (Fam134a), (10ug). Note: ORF is codon optimized
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ORF Plasmid:
tGFP DDK
  $754.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Retreg2
Synonyms Fam134a; MGC47289
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG215599 representing NM_170755, codon optimized.
Due to the complexity of NM_170755, the ORF clone is codon optimized for mammalian Expression.
The nucleotide sequence differs from the reference sequence, yet the amino acid sequence remains identical.

Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCAAGCTCCGGCGGAGGAAATACCGGAGCTGGGGGCACATCAGGCTTGGGCCTGGGACTTGGCCTGT
CCCTGGGGATGGGAGAGGCGACAGGAGATGCAGAGGAAGAAGCTGCTGCTGCTGAGGCCGTGGGCCGGCT
GGCAACCAGTCTGTGGTTGAGGCTTAGAGGCTGGGAAGCTGTCCTTGCAGCTGCTCAGCGGCTCCTGGTG
TGGGAGAAACCTTTGCACTCTCTGGTCACCGCCGCAACCCTGAACGGGCTGTTCTGGCTGCTTAGTAGTT
CAAGCCTCCGCCCCTTTTTCCTCCTCAGTATCTCCCTCTTGACATATTTCCTGTTGGACCTCTGGCACCC
TCGGTTCCTCCCAGACGTAAGCGCTCCTCCACCCGAGGAACCACATTCTGATTCAGAGGGGGCTGGCTCC
GGTGCACAGCCACACCTGCTGTCTGTGCCCGAGCTGTGTCGGTATTTGGCCGAATCCTGGCTGACCTTCC
AAATTCACCTCCAGGAGCTCTTGCAGTACAAGAGACAGAATCCAGCACAGTTTTGCGCTAGGGGATGTGC
CGCATGCGCTGTACTGGCTGTGTTGGGTCATTACGTCCCCGGTGTTATGATCAGTTATATAGTATTGCTC
TCCATCCTGCTGTGGCCGCTGGTCGTCTACCACGAACTTATACAAAGAATGTACACTCGGCTCGAGCCCT
TGCTGATGCAGTTGGATTACTCTATGAAGGCTGAGGCAGACGCTCTCCACCACAAACACGATAAGAGGAA
GCGCCAGGGCAAGTCTGCTCCACCAGCAGGTGATGAACCTCTGGCCGAGACTGAGTCTGAAAGCGAAGCG
GAACTGGCCGGATTCTCACCCGTGGTGGATGTCAAAAAGACTGCGCTGGCATTGGCCATCACCGATAGTG
AACTGTCCGATGAAGAAGCCTCTATCCTGGAGAGCGGGGGTTTCAGTGTATCTCGCGCTACAACACCTCA
GTTGACCGATGTGAGCGAGGATCTGGATCAGCAGAGCCTGCCTTCTGAGCCAGAGGAGGCCCTGAACAGA
GAGTTGGGCGAGGGGGAGGAAACGGAGCTGGCTAGCCCTGAGGATTTGTTGAGTGCACCTCCCGCCCTCT
CCAAACAGGCCCTGGACACCGAGGAGGAAGGAGCTGCCGATAAGGAAGCACTGCTGCAACTCAGCTCCCC
ACTGCACTTCGTGAACACACATTTCAACGGCGCTGGTTCACCTCAGGATGGCGTTAAGTGCCCCCCCGGC
GCTCCAGTTAAGACCCTGTCCCCAGAAGCTGTCAGTGGCGACCTCATGGCTCCCAGCAGCACCCTCAGTC
CCCAATTGTGCCTCGCTGAGAGCGGACCTGTCACCCCGCTCTCTCCCAGTGTGCTTCCTAGTCTTCCTCA
GGATAGCCCCCAACCATTGGCGGCCCCCGAGGAGGAGGAGGCACTCACAACCGAAGACTTTGAACTGCTT
GACCAAGGCGAGCTGGAACAGCTGAACGCGGAGCTCGGACTCGGCCCAGAAATGCCTCCAAAACCCCCTG
ATGTCCTGCCCCCCCCACCCTTGGGCGCTGACAGCCACTCCCTGGTGCAAAGCGACCAGGAGGCACACGC
TGAGGTGGAACCG

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG215599 representing NM_170755
Red=Cloning site Green=Tags(s)

MASSGGGNTGAGGTSGLGLGLGLSLGMGEATGDAEEEAAAAEAVGRLATSLWLRLRGWEAVLAAAQRLLV
WEKPLHSLVTAATLNGLFWLLSSSSLRPFFLLSISLLTYFLLDLWHPRFLPDVSAPPPEEPHSDSEGAGS
GAQPHLLSVPELCRYLAESWLTFQIHLQELLQYKRQNPAQFCARGCAACAVLAVLGHYVPGVMISYIVLL
SILLWPLVVYHELIQRMYTRLEPLLMQLDYSMKAEADALHHKHDKRKRQGKSAPPAGDEPLAETESESEA
ELAGFSPVVDVKKTALALAITDSELSDEEASILESGGFSVSRATTPQLTDVSEDLDQQSLPSEPEEALNR
ELGEGEETELASPEDLLSAPPALSKQALDTEEEGAADKEALLQLSSPLHFVNTHFNGAGSPQDGVKCPPG
APVKTLSPEAVSGDLMAPSSTLSPQLCLAESGPVTPLSPSVLPSLPQDSPQPLAAPEEEEALTTEDFELL
DQGELEQLNAELGLGPEMPPKPPDVLPPPPLGADSHSLVQSDQEAHAEVEP

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_170755
ORF Size 1623 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_170755.2, NP_739561.2
RefSeq Size 2559 bp
RefSeq ORF 1626 bp
Locus ID 227298
UniProt ID Q6NS82
Cytogenetics 1 C4
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Documents
Resources
"NM_170755" in other vectors (4)
SKU Description Size Price
MC218599 Fam134a (untagged) - Mouse family with sequence similarity 134, member A (Fam134a), (10ug) 10 ug
$555.00
MR215599 Fam134a (Myc-DDK-tagged) - Mouse family with sequence similarity 134, member A (Fam134a). Note: ORF is codon optimized 10 ug
$554.00
MR215599L3 Lenti ORF clone of Fam134a (Myc-DDK-tagged) - Mouse family with sequence similarity 134, member A (Fam134a). Note: ORF is codon optimized 10 ug
$854.00
MR215599L4 Lenti ORF clone of Fam134a (mGFP-tagged) - Mouse family with sequence similarity 134, member A (Fam134a). Note: ORF is codon optimized 10 ug
$854.00

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.