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Tle2 (NM_019725) Mouse Tagged ORF Clone

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SKU
MG215597
Tle2 (tGFP-tagged) - Mouse transducin-like enhancer of split 2 homolog of Drosophila E(spl) (Tle2), (10ug)
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ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $902.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Tle2
Synonyms Grg2; mKIAA4188
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG215597 representing NM_019725
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTACCCTCAGGGAAGGCACCCGACCCCGCTGCAGTCTGGCCAGCCTTTCAAGTTCTCAGTACTGGAAA
TCTGTGACCGGATCAAAGAGGAATTCCAGTTTCTTCAAGCTCAGTACCACAGCCTCAAGCTAGAATGTGA
GAAGCTGGCCAGCGAGAAGACAGAAATGCAAAGGCATTATGTGATGGCTGCACCCCATCAGTGTCCCCAG
GGTGGCACCAGCTATCCACACTGGCCAAGACTGTCTCCTTTGCAGTACTACGAGATGTCCTACGGACTCA
ACATTGAGATGCATAAACAGGCTGAGATTGTGAAACGCCTCAGTGCGATCTGTGCCCAGATGGTCCCGTT
CCTCACTCAGGAGCATCAGCAGCAGGTGCTCCAGGCTGTGGACCGAGCCAAGCAGGTGACCGTGGGGGAA
CTGAACAGCCTCCTGGGGCAGCAGAATCAGCTCCAGCCGCTGTCCCACGCACCCCCCGTGCCTCTCACCC
CGCGCCCAGCCGGCCTGGTGGGTGCCGGGGCCACTGGGCTGCTGGCCCTATCTGGGGCACTGGCTGCGCA
GGCCCAGCTGGTGGCTGCCGTAAAGGAAGACCGTGTGGGTGTGGACGCCGAGGGGTCCAGAGTGGACAGA
GCTGCCAGCAGGAGTTCTTCCCCGTCTCCCCCTGAGAGTCTGGTGGAAGAGGACCATCCCAGCAGCCGAG
GCGGCAGTGGGAAACAGCAGAGAGCTGAAGACAAGGATCTGTCAGGGCCTTATGACAGTGAGGAAGACAA
GAGTGACTATAACCTGGTAGTGGATGAGGACCAACCGTCAGAGCCCCCCAGCCCTGTGACCACCCCTTGT
GGGAAGGCGCCCCTCTGCATTCCTGCCCGCAGGGACCTCACAGACAGTCCAGCCTCCTTGGCCTCCAGTT
TGGGCTCACCACTCCCCAGAAGCAAAGACATAGCCCTGAACGATCTTCCCACAGGCACTCCTGCCTCCAG
GTCATGTGGTACCTCTCCACCCCAGGACTCGTCCACCCCGGGGCCCAGCTCAGCCAGTCACCTCTGCCAG
CTGGCGGCTCAGCCGGCAGCACCCACAGACAGCATCGCCCTGAGGAGTCCCCTGACCTTGTCCAGCCCTT
TCACCTCATCCTTCAGCCTGGGCTCCCACAGCACCCTCAATGGGGACCTCTCCATGCCTGGCTCCTATGT
CGGCCTCCACCTGTCCCCCCAGGTCAGCAGCTCTGTCGTGTATGGACGCTCACCTCTGCAGATGGCATTT
GAATCGCACCCCCATCTCCGAGGCTCGTCTGTCTCCTTGCCTGGCATCCCTGTGGCTAAGCCGGCTTACT
CCTTCCACGTGTCTGCGGATGGCCAGATGCAGCCTGTGCCCTTCCCGTCTGATGCTCTGGTAGGCACAGG
CATCCCTCGCCACGCAAGGCAGCTACACACGCTGGCCCACGGTGAGGTGGTGTGTGCCGTCACCATCAGC
AGCTCCACACAGCACGTGTACACAGGCGGCAAAGGATGCGTGAAGGTGTGGGACGTGGGCCAGCCGGGTA
GCAAGACCCCTGTGGCACAGCTGGATTGCCTGAACCGAGACAACTACATCCGCTCCTGCAAGCTGCTGCC
CGACGGGCAGAGCCTGATTGTAGGTGGCGAGGCCAGTACCCTGTCCATTTGGGACCTGGCAGCACCCACA
CCACGGATCAAAGCAGAGCTGACTTCGTCTGCCCCGGCCTGCTATGCTCTGGCCGTCAGTCCGGACGCCA
AGGTCTGCTTCTCCTGCTGCAGCGACGGTAACATCGTGGTCTGGGACCTGCAGAACCAGGCCATGGTCAG
ACAGTTCCAGGGCCACACGGACGGGGCCAGCTGCATCGACATATCAGACTACGGGACCCGGCTGTGGACT
GGGGGCCTGGACAACACTGTGCGCTGCTGGGACCTGCGCGAGGGCCGCCAGCTTCAGCAGCATGACTTCA
GTTCCCAGATTTTCTCATTGGGCCACTGTCCCAATCAGGACTGGTTGGCTGTGGGGATGGAGAGCAGCCA
CGTGGAGGTCCTGCATGTGCGCAAGCCTGAGAAGTACCAGCTCCGCCTCCATGAGAGCTGCGTGCTGTCA
CTCAAGTTCGCTTCCTGTGGACGCTGGTTTGTGAGCACAGGCAAGGACAACCTGCTCAATGCCTGGAGGA
CACCCTATGGGGCCAGCATTTTTCAGTCCAAAGAATCATCTTCTGTACTGAGCTGCGACATCTCCAGGAA
TAATAAGTACATCGTGACAGGCTCAGGGGACAAGAAGGCCACTGTGTATGAGGTGGTGTAC

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG215597 representing NM_019725
Red=Cloning site Green=Tags(s)

MYPQGRHPTPLQSGQPFKFSVLEICDRIKEEFQFLQAQYHSLKLECEKLASEKTEMQRHYVMAAPHQCPQ
GGTSYPHWPRLSPLQYYEMSYGLNIEMHKQAEIVKRLSAICAQMVPFLTQEHQQQVLQAVDRAKQVTVGE
LNSLLGQQNQLQPLSHAPPVPLTPRPAGLVGAGATGLLALSGALAAQAQLVAAVKEDRVGVDAEGSRVDR
AASRSSSPSPPESLVEEDHPSSRGGSGKQQRAEDKDLSGPYDSEEDKSDYNLVVDEDQPSEPPSPVTTPC
GKAPLCIPARRDLTDSPASLASSLGSPLPRSKDIALNDLPTGTPASRSCGTSPPQDSSTPGPSSASHLCQ
LAAQPAAPTDSIALRSPLTLSSPFTSSFSLGSHSTLNGDLSMPGSYVGLHLSPQVSSSVVYGRSPLQMAF
ESHPHLRGSSVSLPGIPVAKPAYSFHVSADGQMQPVPFPSDALVGTGIPRHARQLHTLAHGEVVCAVTIS
SSTQHVYTGGKGCVKVWDVGQPGSKTPVAQLDCLNRDNYIRSCKLLPDGQSLIVGGEASTLSIWDLAAPT
PRIKAELTSSAPACYALAVSPDAKVCFSCCSDGNIVVWDLQNQAMVRQFQGHTDGASCIDISDYGTRLWT
GGLDNTVRCWDLREGRQLQQHDFSSQIFSLGHCPNQDWLAVGMESSHVEVLHVRKPEKYQLRLHESCVLS
LKFASCGRWFVSTGKDNLLNAWRTPYGASIFQSKESSSVLSCDISRNNKYIVTGSGDKKATVYEVVY

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_019725
ORF Size 2301 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_019725.2, NP_062699.1
RefSeq Size 2749 bp
RefSeq ORF 2304 bp
Locus ID 21886
UniProt ID Q9WVB2
Cytogenetics 10 C1
Summary Transcriptional corepressor that binds to a number of transcription factors. Inhibits the transcriptional activation mediated by CTNNB1 and TCF family members in Wnt signaling. The effects of full-length TLE family members may be modulated by association with dominant-negative AES (By similarity).UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_019725" in other vectors (4)
SKU Description Size Price
MC221436 Tle2 (untagged) - Mouse transducin-like enhancer of split 2, homolog of Drosophila E(spl) (Tle2), (10ug) 10 ug
$773.00
MR215597 Tle2 (Myc-DDK-tagged) - Mouse transducin-like enhancer of split 2, homolog of Drosophila E(spl) (Tle2) 10 ug
$589.00 $702.00
MR215597L3 Lenti ORF clone of Tle2 (Myc-DDK-tagged) - Mouse transducin-like enhancer of split 2, homolog of Drosophila E(spl) (Tle2) 10 ug
$1,002.00
MR215597L4 Lenti ORF clone of Tle2 (mGFP-tagged) - Mouse transducin-like enhancer of split 2, homolog of Drosophila E(spl) (Tle2) 10 ug
$1,002.00

file-alt Citations

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