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Npcd (NM_001013360) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG215589
Npcd (tGFP-tagged) - Mouse Cbx6-Nptxr readthrough transcripts (Cbx6-Nptxr) transcript variant 1, (10ug)
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ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $657.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Npcd
Synonyms Cbx6-Nptxr
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG215589 representing NM_001013360
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGCTGTCTGCAGTAGGCGAGCGGGTCTTCGCGGCCGAATCCATCATTAAACGCCGGATCCGCAAGG
GACGCATCGAGTACCTGGTGAAATGGAAAGGGTGGGCGATCAAGTACAGCACTTGGGAGCCAGAGGAGAA
CATTCTGGATTCGAGGCTCATCGCAGCCTTCGAGCAGAAGGAAAGGGAACGTGAGCTGTATGGGCCCAAG
AAGAGGGGACCTAAACCCAAAACTTTCCTCCTAAAGCAGGAGCTCCCAGCCCGTGGGAACATCTCCTCGG
CGCCAGCTCCAGCCATGCCCACCGCCCTGCACTCCAAGATGGACGAACTAGAAGGCCAGCTGCTGGCCAA
GGTGCTGGCGCTGGAGAAGGAGCGAGCCGCCCTCAGTCACGGGAGCCACCAGCAACGACAGGAAGTGGAG
AAGGAGCTGAATGCCCTGCAGGGTCGCGTGGCCGAGCTGGAGCACGGGTCCTCGGCCTACAGTCCCCCTG
ATGCCTTCAAGGTCAGCATCCCCATCCGCAACAACTACATGTACGCCCGCGTGCGCAAGGCGCTGCCGGA
ACTCTACGCCTTCACTGCCTGCATGTGGCTGCGCTCCAGGTCAGGTGGCTCCGGCCAGGGCACCCCCTTC
TCCTACTCGGTGCCCGGGCAGGCCAATGAGATCGTGCTTCTGGAGGCCGGCCTTGAGCCAATGGAGCTGC
TGATCAACGACAAGGTGGCCCAGCTGCCCCTGAGCCTGAAAGACAGCAACTGGCACCATATCTGCATCTC
CTGGACTACAAGGGATGGGCTGTGGTCTGCATACCAGGACGGAGAACTGCGGGGTTCTGGCGAGAACCTA
GCAGCCTGGCACCCCATCAAGCCACACGGCATCCTTATCCTGGGCCAAGAGCAGGATACCTTGGGAGGCC
GATTTGACGCCACACAGGCCTTCGTTGGTGACATTGCACAGTTCAACCTGTGGGACCACGCTCTGACACC
GGCCCAGGTGCTGGGCATGGCCAACTGCACCGGGCCACTGATGGGCAATGTCCTCCCCTGGGAAGATAAG
CTGGTAGAGGCCTTTGGAGGTGCGAAGAAGGCCGCCTTTGATGTGTGCAAGGGGAGGGCAAAGGCA

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG215589 representing NM_001013360
Red=Cloning site Green=Tags(s)

MELSAVGERVFAAESIIKRRIRKGRIEYLVKWKGWAIKYSTWEPEENILDSRLIAAFEQKERERELYGPK
KRGPKPKTFLLKQELPARGNISSAPAPAMPTALHSKMDELEGQLLAKVLALEKERAALSHGSHQQRQEVE
KELNALQGRVAELEHGSSAYSPPDAFKVSIPIRNNYMYARVRKALPELYAFTACMWLRSRSGGSGQGTPF
SYSVPGQANEIVLLEAGLEPMELLINDKVAQLPLSLKDSNWHHICISWTTRDGLWSAYQDGELRGSGENL
AAWHPIKPHGILILGQEQDTLGGRFDATQAFVGDIAQFNLWDHALTPAQVLGMANCTGPLMGNVLPWEDK
LVEAFGGAKKAAFDVCKGRAKA

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001013360
ORF Size 1116 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001013360.2, NP_001013378.2
RefSeq Size 4600 bp
RefSeq ORF 1119 bp
Locus ID 504193
Cytogenetics 15 E1
Summary This gene encodes multiple cytoplasmic proteins composed of a neuronal pentraxin domain linked to a chromo domain. These isoforms are expressed in numerous regions of the central nervous system, where they are present in distinct subcellular arrangements in different brain regions. This gene may play a role in neuronal differentiation. provided by RefSeq, Jul 2008
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Documents
Resources
"NM_001013360" in other vectors (4)
SKU Description Size Price
MC215074 Npcd (untagged) - Mouse neuronal pentraxin chromo domain (Npcd), transcript variant 1, (10ug) 10 ug
$503.00
MR215589 Npcd (Myc-DDK-tagged) - Mouse neuronal pentraxin chromo domain (Npcd), transcript variant 1 10 ug
$289.00 $457.00
MR215589L3 Lenti ORF clone of Npcd (Myc-DDK-tagged) - Mouse neuronal pentraxin chromo domain (Npcd), transcript variant 1 10 ug
$757.00
MR215589L4 Lenti ORF clone of Npcd (mGFP-tagged) - Mouse neuronal pentraxin chromo domain (Npcd), transcript variant 1 10 ug
$757.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.