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H2 (NM_008204) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG215479
H2 (tGFP-tagged) - Mouse histocompatibility 2 M region locus 2 (H2-M2), (10ug)
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ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $500.00
3 Weeks*
Bulk Requests & Clone Modifications

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Other products for "H2 or NM_008204"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol H2
Synonyms H-2M2; Thy19.4
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG215479 representing NM_008204
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGACTTCAGCATTCTTCACTCTCCTCTTCACTCTCTACCTGCTGCTTGGGGCTTCCCTTGCCCTGG
CACAGACCTTTAAAGGATCCCACTCCTTGCGGTACTTCGACATCGCAGTGTCAAGACCTGGCCTAGAGGA
GACCCACTACATGACTGTTGGCTATGTGGATGACACAGAGTTTGTGCATTTTGACAATGAGGCTGAGAAT
CCGAGGTTTGAGCCCCGAGTGCCTTGGATGGAACAGATGGGACAGAAGTACTGGGATGACCAGACACGCA
TTGCGAAAGCTGCAGAACAGCAGATTAGAGTGTACTTTCAGAAACTGCGAGACTACTACAACCAGAGCCA
GAACAGTTCTCACACCATCCAAAGGATGACTGGCTGCTACATTGGACCAGACGGGCACCTACTTCACGCA
TACCGTCAGTTTGGTTATGATGGGCAAGATTACCTCACTCTGAATGAAGATCTGAGCACCTGGACTGCAG
CAGATGCGGCAGCTGAGATCACCCGTAGAGAGTGGGAGGCAACTAATGTGGCTGAGTTCTGGAGGGTCTA
CTTGGAGGGCCCTTGCATGGTGTGGCTCTTTAAATACCTGACAGTGGGAAATGAGACTCTACTGCGCACA
GAGCCCCCAAAGGCATATGTGACCCATCACCCCAGACCTGAAGGTGATGTCACCCTGAGGTGCTGGGCCC
TGGGTTTCTACCCTTCTGACATCATCATGATCTGGCAAAGAGATGGGGAGGACCAGACCCAGGACATGGA
TGTTATTGAGACCAGACCTGCAGGGGATGGAACCTTCCAGAAGTGGGTAGCTGTGGTGGTGCCTTCTGGG
AAGGAGCAGAATTACACATGCCATGTGGCTCATGAGGGACTGCCTGAGCCCCTCACCCTGAGATGGAGTA
AG

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG215479 representing NM_008204
Red=Cloning site Green=Tags(s)

METSAFFTLLFTLYLLLGASLALAQTFKGSHSLRYFDIAVSRPGLEETHYMTVGYVDDTEFVHFDNEAEN
PRFEPRVPWMEQMGQKYWDDQTRIAKAAEQQIRVYFQKLRDYYNQSQNSSHTIQRMTGCYIGPDGHLLHA
YRQFGYDGQDYLTLNEDLSTWTAADAAAEITRREWEATNVAEFWRVYLEGPCMVWLFKYLTVGNETLLRT
EPPKAYVTHHPRPEGDVTLRCWALGFYPSDIIMIWQRDGEDQTQDMDVIETRPAGDGTFQKWVAVVVPSG
KEQNYTCHVAHEGLPEPLTLRWSK

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_008204
ORF Size 912 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_008204.2, NP_032230.1
RefSeq Size 1528 bp
RefSeq ORF 915 bp
Locus ID 14990
Cytogenetics 17 19.16 cM
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Documents
Resources
"NM_008204" in other vectors (4)
SKU Description Size Price
MC208629 H2 (untagged) - Mouse histocompatibility 2, M region locus 2 (H2-M2), (10ug) 10 ug
$330.00
MR215479 H2 (Myc-DDK-tagged) - Mouse histocompatibility 2, M region locus 2 (H2-M2) 10 ug
$289.00 $300.00
MR215479L3 Lenti ORF clone of H2 (Myc-DDK-tagged) - Mouse histocompatibility 2, M region locus 2 (H2-M2) 10 ug
$600.00
MR215479L4 Lenti ORF clone of H2 (mGFP-tagged) - Mouse histocompatibility 2, M region locus 2 (H2-M2) 10 ug
$600.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.