Mark4 (NM_172279) Mouse Tagged ORF Clone

SKU MG215461

Mark4 (tGFP-tagged) - Mouse MAP/microtubule affinity-regulating kinase 4 (Mark4), (10ug)

Product Data

• Type: Mouse Tagged ORF Clone
• Target Symbol: Mark4
• Synonyms: 2410090P21Rik; C79806; Markl1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG215461 representing NM_172279; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGTCTTCGAGGACGGCGCTGGCCCCGGGCAACGATCGAAACTCGGACACGCATGGCACATTGGGCAGTG; GACGATCTTCGGACAAAGGGCCGTCCTGGTCCAGCCGTTCCCTGGGTGCCCGTTGCCGGAACTCTATCGC; TTCCTGCCCTGAGGAACAACCCCATGTGGGCAACTATAGGCTGCTAAGGACCATCGGGAAGGGCAACTTC; GCCAAAGTCAAGCTGGCTCGGCATATCCTCACGGGCCGGGAGGTCGCTATTAAGATCATTGATAAGACCC; AGCTGAACCCCAGTAGCTTGCAGAAGCTGTTCAGAGAAGTCCGAATTATGAAGGGACTCAACCACCCCAA; CATCGTGAAGCTTTTTGAGGTGATAGAGACGGAGAAGACGCTATACCTGGTGATGGAATACGCTAGCGCA; GGAGAAGTGTTTGACTACCTCGTGTCGCACGGCCGCATGAAGGAGAAGGAGGCTCGAGCCAAGTTCCGGC; AGATCGTGTCAGCCGTGCACTACTGTCATCAGAAGAACATTGTACACAGGGATCTAAAGGCTGAAAACCT; GTTGCTGGATGCCGAGGCCAACATCAAAATCGCCGACTTCGGCTTCAGCAATGAGTTCACGCTGGGCTCC; AAGCTGGACACCTTCTGTGGGAGCCCCCCATACGCCGCCCCAGAGCTGTTCCAGGGCAAGAAGTATGATG; GGCCAGAGGTGGACATCTGGAGCCTGGGTGTCATCCTGTACACGCTGGTCAGCGGCTCCCTGCCCTTCGA; TGGGCACAACCTCAAGGAGCTGCGGGAGCGAGTCCTCAGAGGAAAGTACCGGGTCCCCTTCTACATGTCT; ACAGACTGCGAGAGCATTCTGCGGAGATTTCTGGTGCTGAACCCCGCAAAACGCTGTACTCTGGAGCAAA; TCATGAAAGACAAATGGATCAACATCGGCTATGAGGGTGAGGAGCTGAAGCCATACACGGAGCCTGAGGA; GGACTTCGGGGACACCAAGAGAATTGAGGTGATGGTGGGTATGGGCTACACACGGGAAGAAATCAAAGAG; GCCTTGACCAACCAGAAGTACAACGAGGTGACCGCCACCTACCTCCTGCTGGGCAGGAAGACTGAGGAGG; GTGGGGACCGGGGTGCCCCAGGGCTGGCCCTGGCACGGGTGCGGGCGCCCAGCGACACCACCAACGGGAC; AAGCTCCAGCAAAGGCAGCAGCCACAACAAAGGGCAACGGGCTTCTTCCTCCACCTACCACCGCCAGCGC; CGTCACAGTGACTTCTGTGGCCCGTCCCCTGCCCCGCTGCACCCGAAGCGCAGCCCAACCAGCACGGGAG; ACACGGAGCTCAAAGAAGAGCGGATGCCGGGTCGGAAAGCGAGCTGCAGTGCAGTGGGCAGTGGAAGTCG; AGGCTTGCCCCCCTCCAGCCCCATGGTCAGCAGTGCCCACAACCCCAATAAGGCAGAGATCCCTGAGCGG; CGGAAGGACAGCACTAGCACCCCTAACAACCTCCCCCCCAGCATGATGACCCGAAGAAACACCTATGTGT; GCACAGAGCGACCAGGATCTGAACGCCCGTCCTTGTTGCCAAATGGCAAAGAAAATAGCTCCGGTACCTC; GCGGGTGCCCCCTGCCTCGCCTTCCAGTCATAGCCTGGCTCCCCCGTCAGGCGAGCGGAGCCGCCTGGCT; CGGGGCTCCACCATCCGCAGCACCTTCCATGGGGGCCAGGTCCGAGACCGGCGGGCAGGGAGCGGGAGTG; GCGGGGGTGTGCAGAATGGACCCCCAGCCTCACCCACGCTTGCCCACGAGGCCGCACCCCTGCCCTCCGG; GCGGCCTCGCCCCACCACCAACCTCTTCACCAAGCTGACCTCCAAACTGACCCGAAGGGTCACAGACGAA; CCTGAGAGAATCGGGGGACCTGAGGTCACAAGTTGCCATCTACCTTGGGATAAAACGGAAACCGCCCCCA; GGCTGCTCCGATTCCCCTGGAGTGTGAAGCTGACCAGCTCGCGACCTCCTGAGGCCCTGATGGCTGCCCT; GCGACAGGCCACAGCGGCCGCCCGCTGCCGGTGCCGCCAGCCGCAGCCGTTCCTGCTGGCCTGCCTGCAC; GGGGGTGCGGGCGGGCCCGAGCCCCTGTCCCATTTCGAAGTGGAGGTGTGCCAGCTGCCCCGGCCCGGCC; TCAGGGGCGTCCTCTTCCGCCGTGTGGCGGGCACCGCCCTGGCCTTCCGCACCCTCGTCACCCGCATCTC; CAACGACCTCGAACTC; ; ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG215461 representing NM_172279; Red=Cloning site Green=Tags(s); ; MSSRTALAPGNDRNSDTHGTLGSGRSSDKGPSWSSRSLGARCRNSIASCPEEQPHVGNYRLLRTIGKGNF; AKVKLARHILTGREVAIKIIDKTQLNPSSLQKLFREVRIMKGLNHPNIVKLFEVIETEKTLYLVMEYASA; GEVFDYLVSHGRMKEKEARAKFRQIVSAVHYCHQKNIVHRDLKAENLLLDAEANIKIADFGFSNEFTLGS; KLDTFCGSPPYAAPELFQGKKYDGPEVDIWSLGVILYTLVSGSLPFDGHNLKELRERVLRGKYRVPFYMS; TDCESILRRFLVLNPAKRCTLEQIMKDKWINIGYEGEELKPYTEPEEDFGDTKRIEVMVGMGYTREEIKE; ALTNQKYNEVTATYLLLGRKTEEGGDRGAPGLALARVRAPSDTTNGTSSSKGSSHNKGQRASSSTYHRQR; RHSDFCGPSPAPLHPKRSPTSTGDTELKEERMPGRKASCSAVGSGSRGLPPSSPMVSSAHNPNKAEIPER; RKDSTSTPNNLPPSMMTRRNTYVCTERPGSERPSLLPNGKENSSGTSRVPPASPSSHSLAPPSGERSRLA; RGSTIRSTFHGGQVRDRRAGSGSGGGVQNGPPASPTLAHEAAPLPSGRPRPTTNLFTKLTSKLTRRVTDE; PERIGGPEVTSCHLPWDKTETAPRLLRFPWSVKLTSSRPPEALMAALRQATAAARCRCRQPQPFLLACLH; GGAGGPEPLSHFEVEVCQLPRPGLRGVLFRRVAGTALAFRTLVTRISNDLEL; ; TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_172279
• ORF Size: 2256 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_172279.2
• RefSeq Size: 2259 bp
• RefSeq ORF: 2259 bp
• Locus ID: 232944
• UniProt ID: Q8CIP4
• Cytogenetics: 7 A3
• Summary: Serine/threonine-protein kinase (By similarity). Phosphorylates the microtubule-associated protein MAPT/TAU (By similarity). Also phosphorylates the microtubule-associated proteins MAP2 and MAP4 (By similarity). Involved in regulation of the microtubule network, causing reorganization of microtubules into bundles (By similarity). Required for the initiation of axoneme extension during cilium assembly (By similarity). Regulates the centrosomal location of ODF2 and phosphorylates ODF2 in vitro (By similarity). Plays a role in cell cycle progression, specifically in the G1/S checkpoint (By similarity). Reduces neuronal cell survival (By similarity). Plays a role in energy homeostasis by regulating satiety and metabolic rate (PubMed:22992738). Promotes adipogenesis by activating JNK1 and inhibiting the p38MAPK pathway, and triggers apoptosis by activating the JNK1 pathway (PubMed:24989893). Phosphorylates mTORC1 complex member RPTOR and acts as a negative regulator of the mTORC1 complex, probably due to disruption of the interaction between phosphorylated RPTOR and the RRAGA/RRAGC heterodimer which is required for mTORC1 activation (By similarity).[UniProtKB/Swiss-Prot Function]