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Atp2b3 (NM_177236) Mouse Tagged ORF Clone

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SKU
MG215458
Atp2b3 (tGFP-tagged) - Mouse ATPase Ca++ transporting plasma membrane 3 (Atp2b3), (10ug)
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ORF Plasmid:
tGFP DDK
  $1,262.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Atp2b3
Synonyms 6430519O13Rik; Pmca3
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG215458 representing NM_177236
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGCGACATGGCAAACAGTTCCATTGAGTTCCACCCCAAGCCCCAGCAGCAGCGGGAAGTGCCCCATG
TGGGTGGCTTTGGATGCACACTGGCAGAACTACGAAGCCTCATGGAGCTCCGAGGTGCTGAGGCACTGCA
GAAGATTCAAGAAGCCTATGGGGATGTCAGCGGGCTGTGCAGGAGGCTGAAGACCTCACCTACTGAGGGC
CTGGCAGACAACACCAATGACTTGGAGAAACGCAGGCAGATCTATGGGCAGAACTTCATCCCCCCAAAGC
AGCCTAAGACCTTCCTGCAACTGGTGTGGGAAGCCCTGCAGGACGTGACTCTCATCATCCTGGAGGTGGC
TGCTATCGTCTCCCTGGGCCTCTCCTTCTATGCACCTCCTGGAGAGGAAAGTGAAGCCTGTGGGAATGTG
TCTGGTGGGGCAGAGGATGAAGGAGAGGCTGAAGCTGGCTGGATTGAGGGGGCTGCCATCCTACTCTCTG
TCATCTGTGTGGTGCTGGTCACAGCCTTCAATGACTGGAGCAAGGAAAAGCAGTTCCGAGGTCTTCAAAG
CCGTATTGAGCAGGAGCAGAAGTTTACTGTCATACGAAATGGGCAGCTCCTCCAGGTCCCTGTGGCGGCC
CTGGTGGTAGGGGACATTGCCCAGGTCAAATATGGAGATCTTCTGCCTGCCGATGGTGTGCTCATCCAAG
GCAATGACCTCAAGATCGATGAGAGCTCCCTGACCGGCGAGTCGGACCATGTTCGAAAATCAGCAGACAA
AGATCCTATGCTGCTCTCAGGAACTCATGTCATGGAAGGTTCTGGGAGAATGGTGGTAACAGCTGTTGGT
GTGAACTCCCAGACAGGCATCATCTTTACATTGCTTGGGGCAGGTGGAGAGGAGGAGGAGAAGAAAGACA
AGAAAGGCAAGCAGCAGGATGGGGCGATGGACAGTAGCCAAACCAGAGCTAAGAAGCAGGATGGGGCTGT
TGCCATGGAAATGCAACCCCTGAAGAGCGCCGAGGGTGGGGAAATGGAGGAGCGGGAAAAGAAGAAAGCC
AACGTACCCAAGAAGGAGAAGTCAGTCCTGCAAGGAAAGCTCACAAAACTGGCTGTGCAGATTGGGAAAG
CAGGATTGGTGATGTCTGCTATCACTGTCATCATTCTGGTCCTCTACTTTGTGATTGAGACCTTTGTCGT
GGATGGCCGGGTATGGCTGGCAGAGTGCACACCAGTGTATGTGCAGTACTTCGTGAAGTTCTTCATTATT
GGAGTCACTGTTTTGGTTGTGGCTGTCCCTGAGGGCCTGCCTCTTGCTGTTACTATCTCCTTGGCTTATT
CTGTCAAGAAAATGATGAAGGATAATAACCTGGTACGCCACCTGGATGCCTGTGAGACCATGGGCAATGC
CACAGCCATCTGTTCTGACAAGACGGGCACACTCACCACCAACCGCATGACAGTGGTCCAGTCCTACCTA
GGAGACACCCACTACAAAGAGATTCCAGCTCCCAGCGCCCTGACCCCCAAGATTCTTGACCTTCTGGTTC
ACGCCATCTCCATCAACAGTGCCTACACCACCAAAATTCTACCTCCAGAGAAAGAAGGCGCTCTCCCACG
CCAAGTGGGCAACAAAACAGAGTGTGCTCTGCTGGGCTTCGTCTTGGACCTGAAACGTGACTTCCAGCCA
GTACGGGAGCAGATACCAGAAGATCAGCTTTACAAAGTGTACACCTTCAACTCAGTTCGCAAGTCTATGA
GCACAGTTATCCGCATGCCTGATGGTGGCTTCCGCCTCTTTAGCAAGGGAGCCTCAGAGATTCTGCTCAA
AAAGTGTACAAACATCTTAAACAGCAATGGTGAACTCCGAGGATTTCGTCCTCGGGACCGGGATGATATG
GTGAAGAAGATCATTGAGCCTATGGCTTGTGATGGCCTCCGCACCATCTGCATTGCCTACAGGGACTTCT
CTGCTATCCAGGAGCCTGATTGGGACAATGAGAATGAGGTGGTTGGTGACCTTACCTGCATAGCTGTTGT
GGGCATCGAGGACCCTGTGCGACCTGAGGTCCCTGAAGCCATTCGAAAATGCCAGCGTGCTGGCATCACA
GTCCGTATGGTGACTGGAGACAACATCAACACTGCCCGGGCTATTGCAGCTAAGTGTGGCATCATCCAGC
CAGGAGAGGACTTCCTGTGCCTGGAGGGGAAGGAATTCAACAGAAGAATTCGAAATGAGAAAGGCGAGAT
TGAACAGGAGCGGCTGGACAAAGTGTGGCCCAAGCTGCGGGTGCTTGCCCGGTCATCTCCCACTGATAAA
CATACTCTGGTTAAAGGCATTATTGACAGCACAACTGGTGAGCAGCGGCAGGTGGTGGCTGTGACTGGAG
ATGGCACCAATGATGGGCCAGCCCTCAAAAAGGCAGATGTGGGCTTCGCCATGGGCATTGCAGGCACTGA
TGTGGCCAAGGAGGCCTCTGACATCATTCTGACTGATGACAACTTCACCAGCATTGTCAAGGCGGTCATG
TGGGGCCGCAATGTCTATGACAGCATCTCCAAGTTCCTGCAGTTTCAGTTGACAGTCAACGTGGTAGCTG
TGATCGTGGCCTTCACGGGTGCCTGCATTACTCAGGACTCTCCTCTCAAAGCTGTGCAGATGTTGTGGGT
GAACTTAATCATGGACACATTTGCCTCACTTGCCCTGGCAACGGAACCCCCAACTGAGTCACTGCTGCTG
CGGAAGCCATACGGCCGGGACAAGCCTCTCATCTCGAGAACCATGATGAAGAACATCCTTGGACATGCTG
TCTACCAGCTCACCATCATCTTTACCCTGCTATTTGTTGGGGAGCTTTTCTTTGACATTGACAGTGGAAG
AAATGCACCTCTGCACTCACCACCGTCAGAGCACTATACCATCATCTTCAACACGTTCGTCATGATGCAG
CTTTTCAATGAGATCAATGCTCGCAAGATCCATGGTGAGAGGAATGTCTTTGATGGCATCTTCAGCAACC
CCATCTTCTGCACCATTGTCTTGGGCACCTTTGGAATTCAGATTGTCATTGTCCAATTTGGAGGGAAGCC
CTTCAGCTGTTCCCCACTGTCCACAGAACAGTGGCTTTGGTGCCTGTTTGTTGGTGTTGGGGAGCTGGTC
TGGGGACAGGTCATTGCCACCATCCCCACCAGCCAGCTCAAGTGCCTGAAGGAAGCAGGGCATGGGCCTG
GGAAGGATGAGATGACTGATGAAGAGCTGGCCGAAGGGGAAGAAGAAATTGACCATGCTGAACGAGAGCT
CCGCAGGGGCCAGATCCTCTGGTTTCGGGGCCTCAACCGGATCCAGACACAGATCCGGGTGGTGAAAGCA
TTCCGTAGCTCGCTTTATGAAGGCCTGGAGAAACCAGAATCCAAGAGTTGCATCCATAACTTCATGGCAA
CACCCGAGTTTTTGATCAATGACTACACCCACAATATCCCGCTCATCGATGACACAGATGTGGATGAGAA
CGAAGAGCGCCTGAGGGCCCCACCTCCCCCACCCCCTAACCAGAACAACAACGCCATAGACAGCGGCATC
TACCTGACCACGCATGCCACCAAGTCAGCTACCTCTTCAGCATTCTCTTCCAGGCCCGGGAGCCCACTCC
ACAGCATGGAGACATCCCTC

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG215458 representing NM_177236
Red=Cloning site Green=Tags(s)

MGDMANSSIEFHPKPQQQREVPHVGGFGCTLAELRSLMELRGAEALQKIQEAYGDVSGLCRRLKTSPTEG
LADNTNDLEKRRQIYGQNFIPPKQPKTFLQLVWEALQDVTLIILEVAAIVSLGLSFYAPPGEESEACGNV
SGGAEDEGEAEAGWIEGAAILLSVICVVLVTAFNDWSKEKQFRGLQSRIEQEQKFTVIRNGQLLQVPVAA
LVVGDIAQVKYGDLLPADGVLIQGNDLKIDESSLTGESDHVRKSADKDPMLLSGTHVMEGSGRMVVTAVG
VNSQTGIIFTLLGAGGEEEEKKDKKGKQQDGAMDSSQTRAKKQDGAVAMEMQPLKSAEGGEMEEREKKKA
NVPKKEKSVLQGKLTKLAVQIGKAGLVMSAITVIILVLYFVIETFVVDGRVWLAECTPVYVQYFVKFFII
GVTVLVVAVPEGLPLAVTISLAYSVKKMMKDNNLVRHLDACETMGNATAICSDKTGTLTTNRMTVVQSYL
GDTHYKEIPAPSALTPKILDLLVHAISINSAYTTKILPPEKEGALPRQVGNKTECALLGFVLDLKRDFQP
VREQIPEDQLYKVYTFNSVRKSMSTVIRMPDGGFRLFSKGASEILLKKCTNILNSNGELRGFRPRDRDDM
VKKIIEPMACDGLRTICIAYRDFSAIQEPDWDNENEVVGDLTCIAVVGIEDPVRPEVPEAIRKCQRAGIT
VRMVTGDNINTARAIAAKCGIIQPGEDFLCLEGKEFNRRIRNEKGEIEQERLDKVWPKLRVLARSSPTDK
HTLVKGIIDSTTGEQRQVVAVTGDGTNDGPALKKADVGFAMGIAGTDVAKEASDIILTDDNFTSIVKAVM
WGRNVYDSISKFLQFQLTVNVVAVIVAFTGACITQDSPLKAVQMLWVNLIMDTFASLALATEPPTESLLL
RKPYGRDKPLISRTMMKNILGHAVYQLTIIFTLLFVGELFFDIDSGRNAPLHSPPSEHYTIIFNTFVMMQ
LFNEINARKIHGERNVFDGIFSNPIFCTIVLGTFGIQIVIVQFGGKPFSCSPLSTEQWLWCLFVGVGELV
WGQVIATIPTSQLKCLKEAGHGPGKDEMTDEELAEGEEEIDHAERELRRGQILWFRGLNRIQTQIRVVKA
FRSSLYEGLEKPESKSCIHNFMATPEFLINDYTHNIPLIDDTDVDENEERLRAPPPPPPNQNNNAIDSGI
YLTTHATKSATSSAFSSRPGSPLHSMETSL

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_177236
ORF Size 3660 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_177236.4, NP_796210.2
RefSeq Size 4483 bp
RefSeq ORF 3663 bp
Locus ID 320707
Cytogenetics X A7.3
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Documents
Resources
"NM_177236" in other vectors (4)
SKU Description Size Price
MC224004 Atp2b3 (untagged) - Mouse ATPase, Ca++ transporting, plasma membrane 3 (Atp2b3), (10ug) 10 ug
$1,169.00
MR215458 Atp2b3 (Myc-DDK-tagged) - Mouse ATPase, Ca++ transporting, plasma membrane 3 (Atp2b3) 10 ug
$589.00 $1,062.00
MR215458L3 Lenti ORF clone of Atp2b3 (Myc-DDK-tagged) - Mouse ATPase, Ca++ transporting, plasma membrane 3 (Atp2b3) 10 ug
$1,362.00
MR215458L4 Lenti ORF clone of Atp2b3 (mGFP-tagged) - Mouse ATPase, Ca++ transporting, plasma membrane 3 (Atp2b3) 10 ug
$1,362.00

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.