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Tmem185a (NM_001033328) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG215457
BC023829 (tGFP-tagged) - Mouse cDNA sequence BC023829 (BC023829), (10ug)
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ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $500.00
3 Weeks*
Bulk Requests & Clone Modifications

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Other products for "Tmem185a or NM_001033328"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Tmem185a
Synonyms ee3; Tmem185a
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG215457 representing NM_001033328
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAACCTGAGGGGCCTCTTTCAGGACTTCAACCCGAGTAAATTCCTCATCTATGCCTGTCTGCTGCTGT
TCTCTGTGCTACTGGCCCTTCGTTTGGATGGCATCATTCAATGGAGCTACTGGGCTGTCTTTGCTCCAAT
ATGGTTGTGGAAGTTAATGGTCATTGTTGGCGCATCAGTTGGAACTGGAGTTTGGGCACGAAATCCTCAG
TATCGAGCAGAAGGAGAAACATGTGTGGAGTTTAAAGCCATGTTAATTGCTGTGGGCATCCATTTGCTCC
TGCTGATGTTTGAAGTTCTGGTCTGTGACAGGATTGAGAGAGGAAGCCATTTCTGGCTTCTGGTCTTCAT
GCCATTGTTCTTTGTTTCCCCAGTGTCTGTTGCAGCATGTGTCTGGGGCTTTCGACATGACAGGTCACTA
GAGGTTGTGTGTGTCCCTCTGTGGATTCTCATGTCCTTCTTGTGCCTGGTGGTCCTCTATTACATCGTTT
GGTCGGTTCTGTTCCTGCGCTCTATGGATGTGATTGCAGAACAACGCAGAACACATATAACCATGGCACT
GAGCTGGATGACCATTGTCGTGCCTCTTCTTACTTTTGAGATCCTGCTGGTTCACAAACTGGATGGCCAC
AATGCTTTCTCCTGTATTCCAATATTTGTTCCCCTGTGGCTCTCATTGATCACACTGATGGCAACAACAT
TTGGACAGAAAGGAGGAAACCACTGGTGGTTTGGTATTCGCAAAGACTTCTGCCAGTTTCTGCTTGAAAT
ATTCCCGTTTTTGAGAGAGTATGGAAACATTTCATATGATCTCCACCATGAAGATAGTGAGGAAACAGAA
GAAACCCCAGTTCCAGAACCTCCTAAAATTGCACCTATGTTCCGCAAGAAGGCCAGGGTAGTCATCACCC
AGAGCCCTGGCAAGTATGTCCTTCCTCCACCCAAACTAAATATTGAAATGCCAGAC

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG215457 representing NM_001033328
Red=Cloning site Green=Tags(s)

MNLRGLFQDFNPSKFLIYACLLLFSVLLALRLDGIIQWSYWAVFAPIWLWKLMVIVGASVGTGVWARNPQ
YRAEGETCVEFKAMLIAVGIHLLLLMFEVLVCDRIERGSHFWLLVFMPLFFVSPVSVAACVWGFRHDRSL
EVVCVPLWILMSFLCLVVLYYIVWSVLFLRSMDVIAEQRRTHITMALSWMTIVVPLLTFEILLVHKLDGH
NAFSCIPIFVPLWLSLITLMATTFGQKGGNHWWFGIRKDFCQFLLEIFPFLREYGNISYDLHHEDSEETE
ETPVPEPPKIAPMFRKKARVVITQSPGKYVLPPPKLNIEMPD

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001033328
ORF Size 966 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001033328.3
RefSeq Size 1989 bp
RefSeq ORF 969 bp
Locus ID 236848
Cytogenetics X A7.2
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Documents
Resources
"NM_001033328" in other vectors (4)
SKU Description Size Price
MC213027 BC023829 (untagged) - Mouse cDNA sequence BC023829 (BC023829), (10ug) 10 ug
$330.00
MR215457 BC023829 (Myc-DDK-tagged) - Mouse cDNA sequence BC023829 (BC023829) 10 ug
$289.00 $300.00
MR215457L3 Lenti ORF clone of BC023829 (Myc-DDK-tagged) - Mouse cDNA sequence BC023829 (BC023829) 10 ug
$600.00
MR215457L4 Lenti ORF clone of BC023829 (mGFP-tagged) - Mouse cDNA sequence BC023829 (BC023829) 10 ug
$600.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.