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Flrt3 (NM_178382) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG215452
Flrt3 (tGFP-tagged) - Mouse fibronectin leucine rich transmembrane protein 3 (Flrt3) transcript variant 2, (10ug)
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ORF Plasmid:
tGFP tGFP DDK
AAV Particle:
DDK
  $804.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Flrt3
Synonyms 5530600M07Rik; C430047I10Rik; mKIAA1469
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG215452 representing NM_178382
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGATCAGCCCAGCCTGGAGCCTCTTCCTCATCGGGACTAAAATTGGGCTGTTCTTCCAAGTGGCACCTC
TGTCAGTTGTGGCTAAATCCTGTCCATCTGTATGTCGCTGTGACGCAGGCTTCATTTACTGTAACGATCG
CTCTCTGACATCCATTCCAGTGGGAATTCCGGAGGATGCTACAACACTCTACCTTCAGAACAACCAAATA
AACAATGTTGGGATTCCTTCCGATTTGAAGAACTTGCTGAAAGTACAAAGAATATACCTATACCACAACA
GTTTAGATGAATTCCCTACCAACCTTCCAAAGTATGTCAAAGAGTTACATTTGCAAGAGAATAACATAAG
GACTATCACCTATGATTCACTTTCGAAAATTCCGTATCTGGAAGAGTTACACTTGGATGATAACTCAGTC
TCGGCTGTTAGCATCGAAGAGGGAGCATTTCGAGACAGTAACTATCTGCGGCTGCTTTTTCTGTCCCGTA
ACCACCTTAGCACAATCCCGGGGGGCTTGCCCAGGACTATTGAGGAATTACGCCTGGATGACAATCGCAT
ATCAACGATCTCTTCCCCATCACTTCATGGTCTCACAAGCCTGAAACGCCTGGTTTTAGATGGAAACTTG
TTGAACAACCATGGTTTGGGTGACAAAGTTTTCTTCAACTTAGTAAACTTAACAGAGCTGTCCCTGGTGA
GGAATTCCTTGACAGCAGCGCCAGTGAACCTTCCCGGCACAAGCCTGAGGAAGCTTTACCTTCAAGACAA
CCATATCAACCGGGTACCCCCAAATGCTTTTTCTTATTTAAGGCAGCTGTATCGACTCGATATGTCTAAT
AATAACCTAAGCAATTTACCTCAGGGTATCTTTGATGATTTGGACAATATAACCCAACTGATTCTTCGCA
ACAATCCTTGGTATTGTGGATGCAAGATGAAATGGGTACGAGACTGGTTACAGTCGCTACCGGTGAAGGT
CAATGTGCGTGGGCTCATGTGCCAAGCCCCAGAAAAGGTCCGTGGAATGGCTATCAAGGACCTCAGTGCA
GAACTGTTTGATTGTAAAGACAGTGGGATTGTGAGCACCATTCAGATAACCACTGCAATACCCAACACAG
CATATCCTGCTCAAGGACAGTGGCCAGCTCCTGTGACCAAACAACCAGATATTAAAAACCCCAAGCTCAT
TAAGGATCAGCGAACTACAGGCAGCCCCTCACGGAAAACAATTTTAATTACTGTGAAATCTGTCACCCCT
GACACAATCCACATATCCTGGAGACTTGCTCTGCCTATGACTGCTCTGCGACTCAGCTGGCTTAAACTGG
GCCATAGCCCAGCCTTTGGATCTATAACAGAAACAATCGTAACAGGAGAACGCAGTGAATACTTGGTCAC
CGCCCTAGAACCTGAATCACCCTATAGAGTATGCATGGTTCCCATGGAAACCAGTAACCTTTACCTGTTT
GATGAAACACCTGTTTGTATTGAGACCCAAACTGCCCCTCTTCGAATGTACAACCCCACAACCACCCTCA
ATCGAGAGCAAGAGAAAGAACCTTACAAAAATCCAAATTTACCTTTGGCTGCCATCATTGGTGGGGCTGT
GGCCCTGGTAAGCATCGCCCTCCTTGCTTTGGTGTGTTGGTATGTGCATAGGAACGGGTCACTGTTTTCA
CGGAACTGTGCGTACAGCAAAGGGCGGAGGAGAAAGGATGACTATGCAGAAGCCGGTACTAAGAAAGACA
ACTCCATCCTGGAAATCAGGGAAACTTCTTTCCAGATGCTACCGATAAGCAATGAACCCATCTCCAAGGA
GGAGTTTGTAATACACACCATATTTCCTCCGAATGGGATGAATCTGTACAAGAACAACCTCAGTGAGAGC
AGTAGTAACCGGAGCTACAGAGACAGTGGCATCCCAGACTCGGACCACTCACACTCA

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG215452 representing NM_178382
Red=Cloning site Green=Tags(s)

MISPAWSLFLIGTKIGLFFQVAPLSVVAKSCPSVCRCDAGFIYCNDRSLTSIPVGIPEDATTLYLQNNQI
NNVGIPSDLKNLLKVQRIYLYHNSLDEFPTNLPKYVKELHLQENNIRTITYDSLSKIPYLEELHLDDNSV
SAVSIEEGAFRDSNYLRLLFLSRNHLSTIPGGLPRTIEELRLDDNRISTISSPSLHGLTSLKRLVLDGNL
LNNHGLGDKVFFNLVNLTELSLVRNSLTAAPVNLPGTSLRKLYLQDNHINRVPPNAFSYLRQLYRLDMSN
NNLSNLPQGIFDDLDNITQLILRNNPWYCGCKMKWVRDWLQSLPVKVNVRGLMCQAPEKVRGMAIKDLSA
ELFDCKDSGIVSTIQITTAIPNTAYPAQGQWPAPVTKQPDIKNPKLIKDQRTTGSPSRKTILITVKSVTP
DTIHISWRLALPMTALRLSWLKLGHSPAFGSITETIVTGERSEYLVTALEPESPYRVCMVPMETSNLYLF
DETPVCIETQTAPLRMYNPTTTLNREQEKEPYKNPNLPLAAIIGGAVALVSIALLALVCWYVHRNGSLFS
RNCAYSKGRRRKDDYAEAGTKKDNSILEIRETSFQMLPISNEPISKEEFVIHTIFPPNGMNLYKNNLSES
SSNRSYRDSGIPDSDHSHS

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_178382
ORF Size 1947 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_178382.4, NP_848469.1
RefSeq Size 3984 bp
RefSeq ORF 1950 bp
Locus ID 71436
UniProt ID Q8BGT1
Cytogenetics 2 F3
Summary Functions in cell-cell adhesion, cell migration and axon guidance, exerting an attractive or repulsive role depending on its interaction partners (PubMed:19056886, PubMed:25374360). Plays a role in the spatial organization of brain neurons (PubMed:25374360). Plays a role in vascular development in the retina (PubMed:25374360). Plays a role in cell-cell adhesion via its interaction with ADGRL3 and probably also other latrophilins that are expressed at the surface of adjacent cells (PubMed:22405201, PubMed:25374360). Interaction with the intracellular domain of ROBO1 mediates axon attraction towards cells expressing NTN1 (PubMed:24560577). Mediates axon growth cone collapse and plays a repulsive role in neuron guidance via its interaction with UNC5B, and possibly also other UNC-5 family members (PubMed:21673655, PubMed:25374360). Promotes neurite outgrowth (in vitro) (By similarity). Mediates cell-cell contacts that promote an increase both in neurite number and in neurite length (By similarity). Plays a role in the regulation of the density of glutamaergic synapses (PubMed:22405201). Plays a role in fibroblast growth factor-mediated signaling cascades (PubMed:16872596). Required for normal morphogenesis during embryonic development, but not for normal embryonic patterning (PubMed:19056886). Required for normal ventral closure, headfold fusion and definitive endoderm migration during embryonic development (PubMed:18448090). Required for the formation of a normal basement membrane and the maintenance of a normal anterior visceral endoderm during embryonic development (PubMed:19056886).UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_178382" in other vectors (4)
SKU Description Size Price
MG209757 Flrt3 (tGFP-tagged) - Mouse fibronectin leucine rich transmembrane protein 3 (Flrt3) 10 ug
$805.00
MR215452 Flrt3 (Myc-DDK-tagged) - Mouse fibronectin leucine rich transmembrane protein 3 (Flrt3), transcript variant 2 10 ug
$289.00 $604.00
MR215452L3 Lenti ORF clone of Flrt3 (Myc-DDK-tagged) - Mouse fibronectin leucine rich transmembrane protein 3 (Flrt3), transcript variant 2 10 ug
$904.00
MR215452L4 Lenti ORF clone of Flrt3 (mGFP-tagged) - Mouse fibronectin leucine rich transmembrane protein 3 (Flrt3), transcript variant 2 10 ug
$904.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.