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Ctsw (NM_009985) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG215397
Ctsw (tGFP-tagged) - Mouse cathepsin W (Ctsw), (10ug)
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ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $657.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Ctsw
Synonyms lym; lymphopain
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG215397 representing NM_009985
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGACACTGACTGCCCACCTCTCCTACTTTCTGGTCCTGTTGTTAGCGGGCCAAGGCCTCAGTGACTCCC
TCCTCACCAAGGATGCAGGTCCCCGGCCACTGGAGCTGAAGGAAGTCTTCAAGCTGTTCCAGATCCGGTT
CAACCGGAGTTACTGGAACCCAGCAGAGTACACTCGCCGTCTGAGCATCTTTGCCCACAATCTGGCTCAG
GCTCAAAGGCTACAGCAAGAAGACTTGGGTACAGCTGAGTTTGGAGAGACTCCATTCAGTGACCTCACAG
AGGAGGAGTTTGGCCAGTTATACGGGCAGGAGAGGTCACCAGAAAGGACCCCCAACATGACCAAAAAGGT
AGAGTCTAACACGTGGGGGGAATCTGTGCCCCGCACCTGTGACTGGCGTAAAGCAAAGAACATCATCTCG
TCGGTCAAGAACCAGGGAAGCTGCAAATGCTGCTGGGCCATGGCAGCTGCCGACAACATCCAGGCTCTGT
GGCGCATCAAACACCAGCAGTTTGTGGACGTCTCTGTGCAGGAGCTGCTGGACTGCGAACGCTGTGGAAA
TGGTTGCAATGGTGGCTTCGTGTGGGACGCATATCTAACTGTCCTCAACAACAGTGGCCTGGCCAGTGAA
AAGGATTATCCATTCCAGGGGGACAGAAAGCCTCACAGATGCCTAGCCAAGAAGTACAAGAAGGTGGCCT
GGATCCAGGATTTCACCATGTTGTCCAATAATGAGCAGGCAATTGCCCACTACCTGGCCGTGCATGGACC
TATCACCGTGACCATCAACATGAAACTACTCCAGCATTACCAGAAGGGTGTCATCAAGGCTACACCCAGC
TCCTGTGACCCTCGGCAAGTGGACCACTCTGTCTTGCTGGTGGGCTTTGGCAAGGAGAAAGAGGGCATGC
AGACAGGGACAGTCTTGTCCCATTCTCGAAAACGTCGCCACTCCTCCCCATACTGGATCCTGAAGAACTC
CTGGGGAGCTCACTGGGGCGAGAAGGGTTACTTCAGGCTGTATCGGGGAAACAACACCTGTGGAGTCACC
AAGTATCCCTTCACAGCTCAAGTGGACTCACCAGTAAAGAAGGCACGGACCTCTTGTCCTCCC

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG215397 representing NM_009985
Red=Cloning site Green=Tags(s)

MTLTAHLSYFLVLLLAGQGLSDSLLTKDAGPRPLELKEVFKLFQIRFNRSYWNPAEYTRRLSIFAHNLAQ
AQRLQQEDLGTAEFGETPFSDLTEEEFGQLYGQERSPERTPNMTKKVESNTWGESVPRTCDWRKAKNIIS
SVKNQGSCKCCWAMAAADNIQALWRIKHQQFVDVSVQELLDCERCGNGCNGGFVWDAYLTVLNNSGLASE
KDYPFQGDRKPHRCLAKKYKKVAWIQDFTMLSNNEQAIAHYLAVHGPITVTINMKLLQHYQKGVIKATPS
SCDPRQVDHSVLLVGFGKEKEGMQTGTVLSHSRKRRHSSPYWILKNSWGAHWGEKGYFRLYRGNNTCGVT
KYPFTAQVDSPVKKARTSCPP

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_009985
ORF Size 1113 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_009985.5, NP_034115.2
RefSeq Size 1232 bp
RefSeq ORF 1116 bp
Locus ID 13041
UniProt ID P56203
Cytogenetics 19 A
Summary This gene encodes a member of the peptidase C1 (papain) family of cysteine proteases. The encoded preproprotein is proteolytically processed to generate a mature protein product. Expression of the encoded protein is upregulated following lymphocyte activation. Data from a human cell line suggests that the encoded enzyme may be important for viral entry into host cells. provided by RefSeq, Aug 2015
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Documents
Resources
"NM_009985" in other vectors (4)
SKU Description Size Price
MC208360 Ctsw (untagged) - Mouse cathepsin W (Ctsw), (10ug) 10 ug
$503.00
MR215397 Ctsw (Myc-DDK-tagged) - Mouse cathepsin W (Ctsw) 10 ug
$289.00 $457.00
MR215397L3 Lenti ORF clone of Ctsw (Myc-DDK-tagged) - Mouse cathepsin W (Ctsw) 10 ug
$757.00
MR215397L4 Lenti ORF clone of Ctsw (mGFP-tagged) - Mouse cathepsin W (Ctsw) 10 ug
$757.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.