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Epx (NM_007946) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG215396
Epx (tGFP-tagged) - Mouse eosinophil peroxidase (Epx), (10ug)
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ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $856.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Epx
Synonyms E; EPO
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG215396 representing NM_007946
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGATGCAGCAGCTTCTGGCCCTGGTAGGGGCCTTAGCCACACTCATCCTCACCCAACACGCTGAAGGCA
CTGCCCCAGCTTCCCCCAGTCCAGTGGAGATTTCAGTCCTTCGAGACTGCATAGCAGAGGCTAAGCTGCT
GGTGGACACTGCCTACAATCACACACAGAAAAGCATCATGCAACGTCTTCGCAGTGGCTCGGCCAGCCCC
ATGGACCTCCTGGCCTACTTCAAACAGCCAGTAGCAGCCACCAGAAGAGTGGTTCAGGCCGCTGATTATA
TGCATGTGGCCTTAGGGCTGCTAGAAGAGAGGCTGCAGCCACGGGGATCTAGACCCTTCAATGCCACTGA
TGTGCTCACAGAACCACAGCTGCGCCTGCTGTCCCAGGCCAGTGGCTGTGCTCTCCAGGACCAGGCTGAG
AGGTGCAGCAACAAGTACCGAACCATAACAGGGAGATGCAACAACAAGAAGCATCCCTGGCTGGGAGCCT
CCAACCAGGCCCTGGCCCGCTGGCTGCCAGCTGAGTATGAAGACCACAGGTCTCTCCCCTTTGGCTGGAC
CCCTGGAAAGAGGCGTAATGGCTTCCTGCTTCCTCTTGTCCGGGATGTCTCCAATCAGATTGTGCGCTTC
CCCAGCAAGAAACTGACCTCAGACCGCGGCCGGGCCCTTATGTTCATGCAGTGGGGCCAGTTCATCGACC
ATGACCTGGACTTCTCCCCGGAGTCCCCAGCCAGAGTGGCCTTCAGCATGGGTGTGGACTGTGAGAAGAC
CTGTGCCCAGCTGCCCCCCTGCTTCCCCATCAAGATCCCACGCAATGATCCCCGCATCAAGAACCAGCGC
GACTGCATCCCCTTCTTCCGCTCTGCCCCGGCGTGCCCCCAAAACAGGAACAAAGTCCGCAACCAGATCA
ACGCACTTACATCCTTCGTGGACGCCAGCATGGTGTATGGCAGCGAGGTGACCCTCGCCCTGCGGCTCCG
TAATAGGACCAACTTCCTGGGGCTGCTGGCCACGAACCAGCGATTCCAGGACAACGGTCGAGCCCTACTG
CCTTTTGACAACCTGCATGAGGACCCCTGTCTCCTGACTAACCGCTCTGCACGCATCCCCTGCTTCCTGG
CAGGTGACACTCGGTCAAGTGAAACCCCCAAACTGACGGCCTTGCACACGCTGTTTGTGAGAGAACACAA
TCGCCTAGCCGCTGAGCTGAGGCGCCTGAATCCCCACTGGAGTGGAGACAAGCTGTACAACGAAGCCCGG
AAGATCGTGGGAGCCATGGTCCAGATCATCACCTACCGAGACTTCCTGCCTCTGGTTCTGGGCAGGGCCC
GGATCAGAAGAACTTTGGGTCCCTACAGAGGGTATTGCTCCAATGTGGACCCCCGTGTAGCCAATGTTTT
CACCCTGGCCTTCCGCTTTGGCCACACCATGCTTCAGCCCTTCATGTTCCGCCTGGATAGCCAGTATCGG
GCCTCGGCACCCAACTCCCACGTCCCGCTTAGCTCTGTATTCTTTGCCAGCTGGCGAATCATACATGAAG
GTGGCATCGACCCTATCCTCCGAGGTCTCATGGCCACCCCAGCTAAGCTGAACCGGCAGGATTCCATGCT
GGTGGATGAGCTCCGCGACAAGCTGTTTCAGCAAGTGAGAAGGATCGGGCTGGATCTGGCAGCTCTGAAC
ATGCAGCGCAGCCGGGACCACGGCCTTCCAGGATACAATGCCTGGAGACGCTTCTGTGGGCTTTCCCAGC
CCCGAAACTTGGCCCAGCTTAGTCGGGTGCTAAAAAATCAGGATTTGGCAAGGAAGTTTCTGAGACTGTA
CAAGACGCCCGACAACATTGACATCTGGGTCGGAGCCATTGCGGAGCCTCTCCTGCCGGGGGCCCGAGTG
GGGCCGCTTCTGGCTTGTCTTTTTGAGAACCAGTTCAGGAGAGCCAGAGATGGAGACAGATTCTGGTGGC
AGAAATGGGGAGTTTTCACCAAAAGACAACGCAAAGCCCTGAGGCGAATCTCTTTATCTCGGATTGTATG
CGACAATACTGGCATCACCACGGTTTCGAGGGACATCTTCAGGGCTAACATCTACCCACAGGGTTTTGTG
AGCTGTAGCCGTATTCCCAAGTTGAACCTATCAGCCTGGCGAGGCAAA

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG215396 representing NM_007946
Red=Cloning site Green=Tags(s)

MMQQLLALVGALATLILTQHAEGTAPASPSPVEISVLRDCIAEAKLLVDTAYNHTQKSIMQRLRSGSASP
MDLLAYFKQPVAATRRVVQAADYMHVALGLLEERLQPRGSRPFNATDVLTEPQLRLLSQASGCALQDQAE
RCSNKYRTITGRCNNKKHPWLGASNQALARWLPAEYEDHRSLPFGWTPGKRRNGFLLPLVRDVSNQIVRF
PSKKLTSDRGRALMFMQWGQFIDHDLDFSPESPARVAFSMGVDCEKTCAQLPPCFPIKIPRNDPRIKNQR
DCIPFFRSAPACPQNRNKVRNQINALTSFVDASMVYGSEVTLALRLRNRTNFLGLLATNQRFQDNGRALL
PFDNLHEDPCLLTNRSARIPCFLAGDTRSSETPKLTALHTLFVREHNRLAAELRRLNPHWSGDKLYNEAR
KIVGAMVQIITYRDFLPLVLGRARIRRTLGPYRGYCSNVDPRVANVFTLAFRFGHTMLQPFMFRLDSQYR
ASAPNSHVPLSSVFFASWRIIHEGGIDPILRGLMATPAKLNRQDSMLVDELRDKLFQQVRRIGLDLAALN
MQRSRDHGLPGYNAWRRFCGLSQPRNLAQLSRVLKNQDLARKFLRLYKTPDNIDIWVGAIAEPLLPGARV
GPLLACLFENQFRRARDGDRFWWQKWGVFTKRQRKALRRISLSRIVCDNTGITTVSRDIFRANIYPQGFV
SCSRIPKLNLSAWRGK

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_007946
ORF Size 2148 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_007946.2, NP_031972.2
RefSeq Size 2691 bp
RefSeq ORF 2151 bp
Locus ID 13861
UniProt ID P49290
Cytogenetics 11 52.25 cM
Summary This gene encodes a member of the peroxidase superfamily of enzymes that is produced by eosinophils and plays a critical role in eliminating tissue-invasive parasites. The encoded preproprotein undergoes proteolytic processing to generate a heterodimeric enzyme that forms a predominant component of the intracellular granules of eosinophils. Mice lacking the encoded protein exhibit resistance to ulcerative colitis induced by dextran sulfate. provided by RefSeq, Jul 2016
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Documents
Resources
"NM_007946" in other vectors (4)
SKU Description Size Price
MC220986 Epx (untagged) - Mouse eosinophil peroxidase (Epx), (10ug) 10 ug
$657.00
MR215396 Epx (Myc-DDK-tagged) - Mouse eosinophil peroxidase (Epx) 10 ug
$589.00 $656.00
MR215396L3 Lenti ORF clone of Epx (Myc-DDK-tagged) - Mouse eosinophil peroxidase (Epx) 10 ug
$956.00
MR215396L4 Lenti ORF clone of Epx (mGFP-tagged) - Mouse eosinophil peroxidase (Epx) 10 ug
$956.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.