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Pcsk6 (NM_011048) Mouse Tagged ORF Clone

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SKU
MG215393
Pcsk6 (tGFP-tagged) - Mouse proprotein convertase subtilisin/kexin type 6 (Pcsk6), (10ug)
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ORF Plasmid:
tGFP DDK
  $1,078.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Pcsk6
Synonyms b2b2830Clo; C86343; Pace4; SPC4
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG215393 representing NM_011048
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCCTCCGCGCGCGCCGCCAGCGCCCGGGCCCCGGCCGCCGCCCCGGGCCGCCGATGTCGCGCGGGGCG
CAGGGGCCGCCGGCCGGCACGGACTCCCGCCGCTCGCGCTGCGCCCCTGGCGTTGGCTGCTTCTGCTCGC
GCTGCCCGCCGCCTGCTCCGCGCTGCCGCCGCCGCGCCCCGTCTACACCAACCACTGGGCAGTGCAAGTG
CTGGGCGGCCCCGGCGCGGCGGACCGCGTGGCTGCGGCGCACGGCTACCTCAACTTGGGCCAGATTGGAA
ACCTGGACGATTACTATCATTTTTACCACAGCAAGACCTTCAAGAGATCGACCTTGAGCAGCAGGGGCCC
CCACACCTTCCTCAGAATGGACCCACAGGTAAAATGGCTGCAACAACAGGAAGTGAAGCGCAGAGTCAAG
AGACAGGCGCGAAGTGACTCTCTTTATTTCAATGATCCCATTTGGTCTAACATGTGGTACATGCATTGTA
CTGATAAGAACAGTCGCTGTCGGTCAGAGATGAACGTCCAGGCGGCGTGGAAGCGTGGCTACACAGGAAA
GAATGTGGTCGTCACCATCCTCGATGACGGCATAGAAAGGAATCACCCAGACCTGGCCCCCAACTACGAT
TCCTATGCAAGCTACGATGTCAATGGAAATGATTATGACCCATCCCCGAGATATGACGCCAGCAACGAGA
ACAAACATGGCACTCGCTGTGCGGGTGAAGTCGCTGCCTCGGCCAACAACTCCTACTGCATCGTGGGCAT
CGCATATAATGCAAAGATAGGAGGCATCCGGATGCTGGACGGCGATGTGACCGATGTGGTGGAGGCCAAG
TCTCTGGGCATCAGACCCAACTACATTGACATTTACAGCGCCAGTTGGGGACCCGATGATGACGGAAAGA
CAGTAGATGGACCAGGCCGGCTGGCTAAACAAGCTTTCGAGTATGGCATTAAAAAGGGTCGCCAAGGTCT
GGGCTCCATTTTTGTTTGGGCCTCTGGGAATGGTGGGAGAGAAGGGGACCACTGCTCCTGTGATGGCTAC
ACCAACAGCATCTACACCATCTCCGTAAGCAGCACCACTGAGAACGGCCACAAACCCTGGTACCTGGAAG
AATGCGCTTCCACGTTGGCTACCACCTACAGCAGCGGGGCCTTCTATGAACGGAAGATCGTCACCACAGA
TCTGCGTCAGCGCTGCACCGACGGCCACACTGGGACATCAGTCTCAGCCCCCATGGTGGCTGGTATCATT
GCCTTGGCTCTAGAAGCAAACAACCAGTTGACCTGGAGGGATGTGCAGCACCTGCTGGTGAAGACATCAC
GGCCAGCTCATCTGAAGGCGAGCGACTGGAAAGTCAACGGAGCTGGGCATAAAGTTAGCCATCTCTATGG
ATTTGGCTTGGTGGATGCAGAAGCTCTGGTCCTGGAGGCGAGGAAGTGGACGGCAGTGCCCTCCCAGCAC
GTATGCGTGGCCACCGCAGACAAAAGGCCCAGGAGCATCCCCATAGTGCAGGTGCTACGGACCACAGCCC
TGACCAACGCCTGTGCGGACCACTCAGACCAGCGTGTGGTGTACCTGGAGCATGTGGTTGTCCGAATCTC
TATCTCACACCCACGCCGGGGCGATCTCCAGATCCACCTGATTTCTCCCTCTGGAACCAAGTCTCAACTT
TTGGCAAAGAGATTGCTGGATTTTTCCAATGAGGGGTTCACAAACTGGGAATTCATGACCGTCCACTGCT
GGGGAGAAAAGGCTGAAGGGGAGTGGACTCTGGAAGTGCAGGATATACCATCGCAGGTCCGAAACCCAGA
GAAACAAGGAAAGCTGAAAGAATGGAGCCTCATTTTATATGGCACCGCAGAGCACCCATATCGCACCTTC
AGCTCCCATCAGTCTCGCTCACGGATGCTGGAGCTCTCCGTCCCGGAGCAGGAGCCTCCCAAGGCTGCTG
GACAACCACCCCAGGCAGAGACTCCAGAAGACGAGGAAGAGTACACAGCTCCTTCCATCCAAGGCTCTCC
TAATACTTTACAGACCAGTGTGTGCCATCCCGAGTGTGGTGACAAAGGCTGTGATGGTCCCAATGCAGAC
CAGTGCTTGAACTGTGTCCACTTCAGCCTGGGGAACTCCAAGACAAACAGGAAGTGTGTGAGCGAGTGCC
CCTTGGGCTACTTTGGGGATGCAGCTGCAAGACGCTGCCGTCGCTGCCATAAGGGATGTGAGACGTGCAC
GGGCAGGAGCCCAGCACAGTGCCTGTCTTGTCGCCGTGGGTTCTATCACCACCAGGAGACGAACACGTGT
GTGACCCTCTGTCCTGCCGGACTTTATGCTGATGAAAGTCAGAGACTCTGCCTCAGGTGCCACCCGAGCT
GTCAGAAGTGCGTGGATGAACCTGAGAAGTGCACTGTGTGTAAGGAGGGATTCAGCCTGGCACGGGGCAG
CTGCATTCCGGACTGTGAACCGGGTACCTACTTCGATTCAGAGCTCGTCAAATGTGGGGAATGCCATCAC
ACCTGCCGGACCTGTGTGGGGCCCAGCAGAGAAGAGTGTATTCACTGTGCAAAAAGCTTCCACTTCCAAG
ACTGGAAATGTGTGCCGGCCTGCGGTGAGGGCTTCTACCCGGAGGAGATGCCTGGCTTACCCCACAAAGT
GTGTCGAAGATGTGAGGAGAACTGCCTGAGCTGCGAGGGCTCCAGTAGGAACTGCAGCAGATGTAAAGCC
GGCTTCACACAGCTGGGAACCTCCTGCATCACCAACCACACGTGCAGCAATGCCGATGAGACCTTCTGCG
AGATGGTGAAGTCCAATCGGCTCTGCGAACGGAAGCTCTTCATCCAGTTTTGCTGCCGCACCTGCCTCCT
GGCTGGG

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG215393 representing NM_011048
Red=Cloning site Green=Tags(s)

MPPRAPPAPGPRPPPRAADVARGAGAAGRHGLPPLALRPWRWLLLLALPAACSALPPPRPVYTNHWAVQV
LGGPGAADRVAAAHGYLNLGQIGNLDDYYHFYHSKTFKRSTLSSRGPHTFLRMDPQVKWLQQQEVKRRVK
RQARSDSLYFNDPIWSNMWYMHCTDKNSRCRSEMNVQAAWKRGYTGKNVVVTILDDGIERNHPDLAPNYD
SYASYDVNGNDYDPSPRYDASNENKHGTRCAGEVAASANNSYCIVGIAYNAKIGGIRMLDGDVTDVVEAK
SLGIRPNYIDIYSASWGPDDDGKTVDGPGRLAKQAFEYGIKKGRQGLGSIFVWASGNGGREGDHCSCDGY
TNSIYTISVSSTTENGHKPWYLEECASTLATTYSSGAFYERKIVTTDLRQRCTDGHTGTSVSAPMVAGII
ALALEANNQLTWRDVQHLLVKTSRPAHLKASDWKVNGAGHKVSHLYGFGLVDAEALVLEARKWTAVPSQH
VCVATADKRPRSIPIVQVLRTTALTNACADHSDQRVVYLEHVVVRISISHPRRGDLQIHLISPSGTKSQL
LAKRLLDFSNEGFTNWEFMTVHCWGEKAEGEWTLEVQDIPSQVRNPEKQGKLKEWSLILYGTAEHPYRTF
SSHQSRSRMLELSVPEQEPPKAAGQPPQAETPEDEEEYTAPSIQGSPNTLQTSVCHPECGDKGCDGPNAD
QCLNCVHFSLGNSKTNRKCVSECPLGYFGDAAARRCRRCHKGCETCTGRSPAQCLSCRRGFYHHQETNTC
VTLCPAGLYADESQRLCLRCHPSCQKCVDEPEKCTVCKEGFSLARGSCIPDCEPGTYFDSELVKCGECHH
TCRTCVGPSREECIHCAKSFHFQDWKCVPACGEGFYPEEMPGLPHKVCRRCEENCLSCEGSSRNCSRCKA
GFTQLGTSCITNHTCSNADETFCEMVKSNRLCERKLFIQFCCRTCLLAG

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_011048
ORF Size 2877 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_011048.1, NP_035178.1
RefSeq Size 4298 bp
RefSeq ORF 2880 bp
Locus ID 18553
Cytogenetics 7 35.36 cM
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Documents
Resources
"NM_011048" in other vectors (4)
SKU Description Size Price
MC222828 Pcsk6 (untagged) - Mouse proprotein convertase subtilisin/kexin type 6 (Pcsk6), (10ug) 10 ug
$967.00
MR215393 Pcsk6 (Myc-DDK-tagged) - Mouse proprotein convertase subtilisin/kexin type 6 (Pcsk6) 10 ug
$589.00 $878.00
MR215393L3 Lenti ORF clone of Pcsk6 (Myc-DDK-tagged) - Mouse proprotein convertase subtilisin/kexin type 6 (Pcsk6) 10 ug
$1,178.00
MR215393L4 Lenti ORF clone of Pcsk6 (mGFP-tagged) - Mouse proprotein convertase subtilisin/kexin type 6 (Pcsk6) 10 ug
$1,178.00

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.