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Mcf2 (NM_133197) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG215353
Mcf2 (tGFP-tagged) - Mouse mcf.2 transforming sequence (Mcf2), (10ug)
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ORF Plasmid:
tGFP DDK
  $1,050.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Mcf2
Synonyms B230117G22Rik; Dbl; Mcf-2
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG215353 representing NM_133197
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCAGAAGCAAATCCCCCGAGAGGCAAGATGAGGTTCAGAAGGAATGCGGCCTCCTTCCCTGGGAGCT
TACACTTAGTCCTGGTTTTGCGTCCTACCAGTTTTCTTCAACGGACTTTCACGGACATTGGATTTCGATT
TAGCCAGGAGGATTTTATGCTGAAATTACCAGTTGTTATGCTGAGCTCAGTTAGTGATTTGCTGACATAC
ATTGATGACAAGCAGTTAACCCCTGAGTTAGGCGGCACCTTGCAGTACTGCCACAGTGAATGGATCATCT
TCAGAAATGCCATAGAGAAGTTTGCTGTCACAGTGAAAGAAATGGCTCAGATGTTGCAATCCTTTGGAAC
AGAATTGGCTGAAACAGAGCTACCACAGGATATCCTTTCAATAGAAGAGATTCTGGCAGGTCGAGCCGAA
AGGTATCACCTGTTGAAGAATGACTTAACTGCTGTAACCAAAGAAGGAAAAGTTCTGCTCATGAACCTGC
AAGTGCCTGCCACTGAAGAAACTGTCAGTTCAAGCCTTGAATGTACTCAGCACATAAATGGCGACTGGCA
AACTATTAAAAAGTTGCTGGCTCAGGTGCATGATATGGAAACTGCTTTTGATGGATTTTGGGAAAAACAT
CAGCTGAAAATGGAGCAGTATCTGCAACTGTGGAAGTTTGAACAAGACTTTCAAGAGGCTGTGACTCAAG
TTGAATTTTTATTAAGTCAACAAAGAGAACTGGGTGATATAACAGGGAACTTAGCTCAAGTAAAACAAAG
ACTTAAAAAACTAGAGATCTTAGATGATAAATCTCAGGAACTGTTAACCAAGGCCCGGATTGTGATACTA
CGTGGCCACAAGCTGGCATCGAATCACCACTATGCACTTGATTTAATTTGCCAGCGATGCAATGAACTAC
GCTACCTTTCTGATATTTTAGTTAATGAGATCAGAACAAAGCGGGTCCAACTGAGCAGGACTTTCAAAGT
GCATAGACTCTTACAGCAGGCACGTCAGTGCTGTGATCAAGGGGAATGCCTCCTGGCTAGCCAAGGAATG
GATAAGTTTCAGACTAAAGAAGATGCTCAGAAAGCCCTTCAGGATGTGGACAATTTCCTTCAAATGGCCA
TGCCTTTCATCAATTATGATATCGAAAGCCTGCAATATGAATTTGATGTACTCCTATCTCCTGAACTCAA
GGCTCAAATGCAGAATATACAACTAAAGCTTGAAAATATTCGAAGTGCATTTCAGAACCAACAGGCTGGT
TGTAAGAGCCTAAAAGAAGTACCCGAGGGGGCGTTCCAGAACTTGGTTCCTGCATCTGAAAATGTGATGC
GGTCCAGAATGATATTCTTTTCACCGAAACATGTGAAAAAGTCTTGGAGACAGATTCACGCTCAGAGCAA
TGTGAAAGTTGAAGCCGTTGAGGATAGCCAAGAGAAGAATTCTGATCAGTCTCCCAAATTGGACAATAGC
TTGGATATTTTAAAGAACCATGTCCTAAATGAGCTGATACAGACAGAGAGAGCATATGTTCGAGAGCTGT
TTACTGTTTTGTTGGGCTACAGATCTGAGATGGACAATCCACAGATGTTTGATCTTATGCCACCTCTCCT
GAGAAATAAAAAGGATGTTCTCTTTGGAAATATGGCAGAAATATACGAATTCCATAACAACATTTTCATG
AGCAGACTGGAAGATTGCTCTGATGCTCCAGAAAGAGTGGGACCTTGCTTCCTGGAAAGGAAGGATGATT
TTCAGATGTATGCCAAATATTGTCAGAATAAACCCAGGTCAGAGTTAATTTGGAGGAAGTATTCTGAATG
TGCCTTTTTCCAGGAATGTCAACGAAAATTGAAACATCGACTTGGTCTGGATTCCTATTTGCTCAAACCT
GTTCAACGAATCACTAAGTATCAGTTGCTTTTAAAGGAGCTTTTAAAGTATAGCAAAGAAGGTGAAGGAA
CTACTAAATTAAAGGAGGCACTTGACTCAATGCTGGAGCTGCTGAAGTCAGTGAATGACTCCATGCATCA
GACTGCCATCAATGGCTATGTTGGAAATATAAATGAGCTGGGCAAGATGGTTCTTCAAGGTTCCTTCAAT
GTTTGGCTAGGACACCGGAAAGGTGCTACAAAAATGAAGGATTTTGCACGTTTCAAACCCATGCAGAGAC
ATCTTTTCTTATATGAAAAAGCTGTTATGTTTTGTAAGAGGCGTTTCGAATCTGGAGAAGGTGCTGATAG
ATACCCATCATACAGTTTTAAGCACTGCCTAAAAATGGAAGATGTTGGCATCACAGAACATGTGAAAGGT
GATAACCGCAAATTTGAAATACGGTATAGTGAGAAGGAAGAAATTTACATTGTTCAGGCTCCAAATGTTG
ATGTGAAGATGTTATGGCTAAAAGAAATAAGAAAAATTTTGGTGCAGCAGAAGGAACTTATGACTGCTAA
AACCCAGCAGGATCAGGCACTAGACCAGGACCAACTTTTCCCCCAGCAGCAGAATGCCGAGCTATGCAAA
AGCTCACCTTTTTGTGTGTGTGAGGAAACTCTTTTCAACGCCACAGAAGCAGGAGCAGAAGTGGAGCAAG
CTGGTGCCCTGATAAAGGTCAGGGAGGCAGCTGTGCTAGCTCAAGCAGAGGCAAGTTCAGCAGCTTGGAA
TGGGATGTTACCATCTGCAGAAGGCGCTGCTGCAATTGCTGAACATTCATACAATTATTTCTCCTCTAAC
AATGACCATGGAGAAGATAGGACACAAATGAGACACATGTCGGAGGTGACATTC

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG215353 representing NM_133197
Red=Cloning site Green=Tags(s)

MAEANPPRGKMRFRRNAASFPGSLHLVLVLRPTSFLQRTFTDIGFRFSQEDFMLKLPVVMLSSVSDLLTY
IDDKQLTPELGGTLQYCHSEWIIFRNAIEKFAVTVKEMAQMLQSFGTELAETELPQDILSIEEILAGRAE
RYHLLKNDLTAVTKEGKVLLMNLQVPATEETVSSSLECTQHINGDWQTIKKLLAQVHDMETAFDGFWEKH
QLKMEQYLQLWKFEQDFQEAVTQVEFLLSQQRELGDITGNLAQVKQRLKKLEILDDKSQELLTKARIVIL
RGHKLASNHHYALDLICQRCNELRYLSDILVNEIRTKRVQLSRTFKVHRLLQQARQCCDQGECLLASQGM
DKFQTKEDAQKALQDVDNFLQMAMPFINYDIESLQYEFDVLLSPELKAQMQNIQLKLENIRSAFQNQQAG
CKSLKEVPEGAFQNLVPASENVMRSRMIFFSPKHVKKSWRQIHAQSNVKVEAVEDSQEKNSDQSPKLDNS
LDILKNHVLNELIQTERAYVRELFTVLLGYRSEMDNPQMFDLMPPLLRNKKDVLFGNMAEIYEFHNNIFM
SRLEDCSDAPERVGPCFLERKDDFQMYAKYCQNKPRSELIWRKYSECAFFQECQRKLKHRLGLDSYLLKP
VQRITKYQLLLKELLKYSKEGEGTTKLKEALDSMLELLKSVNDSMHQTAINGYVGNINELGKMVLQGSFN
VWLGHRKGATKMKDFARFKPMQRHLFLYEKAVMFCKRRFESGEGADRYPSYSFKHCLKMEDVGITEHVKG
DNRKFEIRYSEKEEIYIVQAPNVDVKMLWLKEIRKILVQQKELMTAKTQQDQALDQDQLFPQQQNAELCK
SSPFCVCEETLFNATEAGAEVEQAGALIKVREAAVLAQAEASSAAWNGMLPSAEGAAAIAEHSYNYFSSN
NDHGEDRTQMRHMSEVTF

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_133197
ORF Size 2784 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_133197.5
RefSeq Size 3617 bp
RefSeq ORF 2787 bp
Locus ID 109904
Cytogenetics X 33.5 cM
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Documents
Resources
"NM_133197" in other vectors (4)
SKU Description Size Price
MC222652 Mcf2 (untagged) - Mouse mcf.2 transforming sequence (Mcf2), (10ug) 10 ug
$936.00
MR215353 Mcf2 (Myc-DDK-tagged) - Mouse mcf.2 transforming sequence (Mcf2) 10 ug
$589.00 $850.00
MR215353L3 Lenti ORF clone of Mcf2 (Myc-DDK-tagged) - Mouse mcf.2 transforming sequence (Mcf2) 10 ug
$1,150.00
MR215353L4 Lenti ORF clone of Mcf2 (mGFP-tagged) - Mouse mcf.2 transforming sequence (Mcf2) 10 ug
$1,150.00

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.