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Setd1a (NM_178029) Mouse Tagged ORF Clone

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SKU
MG215352
Setd1a (tGFP-tagged) - Mouse SET domain containing 1A (Setd1a), (10ug). Note: ORF is codon optimized
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ORF Plasmid:
tGFP DDK
  $1,642.00
3 Weeks*
Bulk Requests & Clone Modifications

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Other products for "Setd1a or NM_178029"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Setd1a
Synonyms BC010250; KMT2F; mKIAA0339; mNSC1; Nsccn1
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG215352 representing NM_178029, codon optimized.
Due to the complexity of NM_178029, the ORF clone is codon optimized for mammalian Expression.
The nucleotide sequence differs from the reference sequence, yet the amino acid sequence remains identical.

Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGACCAGGAAGGTGGGGGAGACGGGCAGAAAGCCCCGAGCTTCCAGTGGCGGAACTACAAGCTCATAG
TGGATCCTGCTTTGGACCCTGCTTTGCGCAGGCCTTCCCAGAAGGTGTACCGCTATGACGGAGTCCACTT
CAGTGTCAGTGACTCAAAGTATACCCCCGTGGAAGACCTCCAGGACCCCCGCTGTCATGTCAGGTCCAAA
GCCAGAGACTTTTCCCTCCCGGTTCCTAAGTTTAAGCTGGATGAGTTCTATATTGGACAGATACCACTGA
AGGAAGTAACTTTTGCGAGGCTCAATGACAATGTGAGGGAAACCTTCCTGAAGGATATGTGCCGGAAATA
TGGAGAGGTGGAAGAAGTGGAGATCCTTCTTCATCCCCGCACTCGAAAGCACCTAGGCCTGGCCCGTGTG
CTCTTCACCAGCACTCGGGGCGCCAAGGAAACTGTCAAAAACCTCCACCTCACCTCTGTCATGGGCAACA
TCATTCATGCCCAGCTTGATATCAAAGGCCAACAACGAATGAAGTACTATGAATTGATTGTCAATGGCTC
TTACACTCCTCAGACTGTGCCCACTGGGGGCAAGGCCTTGAGTGAGAAGTTCCAGGGTTCTGGTGCAGCC
GCTGAGACGACTGAAGCCCGCCGACGGTCTTCCTCGGACACAGCTGCCTATCCAGCAGGCACTACTGTGG
GGGGCACCCCTGGCAATGGCACTCCCTGCTCCCAAGATACAAATTTCTCCAGCAGCCGACAAGATACTCC
ATCTTCCTTTGGCCAGTTCACCCCTCAGTCCTCCCAAGGAACCCCCTACACATCTCGGGGTAGCACCCCC
TACTCTCAGGACTCTGCCTACTCCAGCAGCACCACTTCAACCTCCTTCAAACCCCGGCGGTCAGAGAACA
GCTATCAAGATTCTTTTTCTCGCCGCCATTTCTCTACATCTTCAGCCCCTGCAACCACCGCTACTGCCAC
CTCAGCAACTGCTGCAGCTACTGCAGCCTCCTCCTCATCTTCCTCCTCCTCGTCATCCAGCAGCAGCAGC
AGCTCTAGCTCTTCAGCCTCCCAGTTTAGAGGCTCCGACTCATCCTATCCTGCCTATTATGAATCATGGA
ACAGATATCAAAGGCATACTTCCTACCCTCCGCGCCGCGCCACCCGCGAGGATCCGTCCGGAGCTAGCTT
TGCTGAGAACACAGCAGAGCGCTTTCCTCCCAGTTACACGTCCTATCTTGCCCCTGAGCCAAATCGGTCA
ACTGACCAAGACTACCGACCTCCCGCCAGTGAAGCTCCCCCACCAGAGCCACCTGAGCCTGGAGGAGGTG
GAGGGGGGAGTGGCGGGGGGGGCGGCGGAGGGGGGGGCGGTGGAGGCGGGGCTCCATCTCCAGAACGGGA
GGAAGCACGCACGCCTCCCCGCCCAGCCAGCCCCGCAAGGAGCGGTTCTCCCGCTCCTGAGACAACAAAC
GAGAGCGTACCCTTTGCACAACACTCTTCTCTCGACTCCCGCATTGAGATGTTGTTGAAGGAGCAGAGGA
GCAAATTCAGTTTTTTGGCATCTGACACCGAAGAAGAGGAGGAGAATTCTTCAGCCGGGCCGGGCGCCCG
GGATGCTGGTGCCGAGGTGCCAAGCGGAGCCGGACATGGACCCTGCACACCGCCCCCCGCCCCAGCGAAC
TTTGAAGATGTCGCCCCGACAGGGTCTGGGGAACCAGGAGCCGCAAGGGAAAGCCCCAAAGCAAACGGGC
AAAATCAGGCCTCTCCCTGTTCATCTGGCGAGGACATGGAAATCTCAGATGACGACAGAGGCGGATCCCC
ACCCCCCGCCCCAACACCCCCACAGCAGCCTCCACCTCCACCACCACCTCCTCCACCTCCGCCACCACCC
TACCTGGCCTCACTGCCTTTGGGATACCCACCCCACCAGCCAGCTTACCTTCTGCCCCCGCGACCTGATG
GTCCCCCCCCCCCGGAATACCCTCCCCCACCCCCGCCACCACCCCCCCATATTTATGACTTCGTAAATTC
TCTGGAACTTATGGACCGGCTGGGCGCCCAGTGGGGAGGGATGCCAATGTCCTTTCAGATGCAGACACAG
ATGCTGACAAGACTGCATCAGCTTCGCCAAGGGAAGGGGCTCACGGCTGCAAGCGCTGGGCCGCCTGGGG
GGGCTTTCGGCGAGGCCTTTTTGCCTTTCCCACCTCCACAAGAAGCAGCCTACGGACTGCCCTATGCTCT
GTACACCCAAGGACAGGAGGGGCGAGGCTCTTATAGCCGCGAGGCCTACCATCTTCCTTTGCCTATGGCT
GCTGAACCGTTGCCCAGCTCTTCCGTGTCAGGGGAGGAAGCCAGACTCCCGCACCGCGAAGAGGCTGAGA
TTGCAGAATCCAAGGTGTTGCCTTCCGCTGGAACAGTGGGGCGGGTGTTGGCCACACTTGTGCAGGAAAT
GAAGTCTATCATGCAGCGAGATCTGAACAGGAAAATGGTGGAGAACGTCGCATTTGGCGCCTTCGACCAG
TGGTGGGAATCAAAAGAAGAGAAAGCTAAGCCCTTTCAGAACGCAGCCAAGCAGCAGGCGAAGGAGGAGG
ATAAAGAGAAGATGAAACTGAAAGAACCTGGCATGCTGTCTCTCGTCGACTGGGCTAAAAGCGGAGGGAT
CACCGGAATTGAGGCTTTCGCTTTTGGCAGCGGTCTGCGGGGGGCACTCCGCCTGCCGAGTTTCAAAGTA
AAGAGGAAGGAGCCAAGCGAGATCTCCGAGGCGAGCGAAGAGAAACGCCCTAGGCCATCAACACCTGCCG
AAGAGGACGAAGACGATCCAGAAAGAGAGAAGGAAGCTGGGGAGCCAGGCCGCCCTGGAACTAAACCGCC
AAAGAGAGACGAGGAGCGCGGGAAGACGCAGGGCAAGCACAGGAAGTCTTTTACCTTGGATTCTGAAGGT
GAGGAGGCGTCCCAGGAGTCTTCCTCAGAGAAGGATGAGGATGATGATGATGAAGATGAGGAAGATGAAG
AACAGGAGGAAGCTGTGGATGCCACCAAGAAGGAAGCAGAGGCATCGGATGGTGAGGATGAGGACAGTGA
CTCGTCCTCCCAGTGCTCTCTGTATGCGGATTCAGATGGTGAAAATGGCAGCACATCAGACTCCGAGAGT
GGCAGCTCGTCTTCCTCTTCCTCATCGTCTTCCTCCTCTTCCTCCTCTTCCTCATCAGAGTCCTCCTCTG
AGGAAGAGGAGCAGTCAGCAGTCATTCCCTCAGCTTCACCCCCCAGAGAAGTTCCAGAGCCCCTTCCAGC
TCCTGATGAGAAGCCTGAGACAGACGGGCTTGTAGATTCCCCTGTCATGCCTCTCTCTGAAAAGGAGACA
TTGCCCACACAGCCTGCAGGCCCTGCTGAAGAACCACCTCCTAGTGTACCACAGCCTCCAGCAGAGCCAC
CCGCCGGACCGCCAGACGCTGCCCCCCGCCTGGATGAGCGCCCCTCTTCTCCTATCCCTCTCCTACCCCC
ACCCAAGAAACGCCGGAAAACTGTTTCCTTCTCTGCTGCAGAGGAGGCACCAGTCCCAGAACCTTCCACA
GCTGCCCCACTGCAGGCCAAGTCTTCTGGTCCAGTCTCTCGAAAAGTCCCCCGGGTCGTAGAGCGGACCA
TTCGTAACCTCCCCCTGGACCATGCGTCTCTGGTCAAGAGTTGGCCTGAGGAGGTCGCCCGAGGGGGTCG
GAATCGAGCTGGAGGTCGTGTGCGCTCTACTGAAGAAGAGGAGGCCACTGAGTCAGGGACAGAAGTAGAC
CTGGCAGTACTGGCTGACCTGGCCCTGACCCCAGCCCGACGTGGGTTGGCTACCCTACCCACTGGTGACG
ACTCAGAGGCCACAGAGACCTCAGATGAGGCTGAGCGCCCAAGCCCTCTACTCAGCCACATCCTCTTGGA
ACACAACTATGCCCTAGCCATCAAGCCCCCGCCTACCACACCAGCACCTCGGCCCCTGGAGCCAGCTCCT
GCCCTTGCTGCACTCTTCAGCTCTCCAGCTGATGAAGTCTTAGAGGCCCCTGAGGTGGTGGTGGCTGAGG
CAGAGGAGCCAAAGCAACAGCTGCAGCAGCAGCATCCAGAGCAGGAAGGAGAAGAGGAGGAGGAAGATGA
AGAGGAGGAGTCCGAGTCTTCAGAAAGCAGCAGCAGCAGCAGCAGTGATGAGGAAGGGGCAATCAGGAGG
CGTAGCCTCCGCTCCCACACTCGAAGAAGAAGGCCACCACTCCCACCCCCACCCCCACCTCCTCCTTCCT
TTGAGCCGAGAAGTGAATTTGAGCAGATGACCATCTTGTATGATATTTGGAACTCAGGCCTTGACTTGGA
AGACATGAGCTATTTGCGACTCACATATGAACGGCTGCTGCAGCAAACCAGTGGCGCTGACTGGCTTAAT
GACACCCACTGGGTCCAGCACACAATCACCAACCTAAGCACTCCAAAGCGCAAAAGACGGCCCCAAGATG
GGCCCCGGGAGCATCAGACAGGCTCTGCACGCAGCGAAGGTTATTACCCCATTAGCAAGAAGGAGAAAGA
TAAATACTTGGATGTGTGCCCCGTCTCGGCCCGGCAGCTGGAAGGTGGAGACACTCAGGGGACTAATCGG
GTGCTTTCTGAGCGACGGTCAGAGCAGCGGCGGTTACTAAGCGCTATTGGCACCTCAGCCATCATGGACA
GTGATCTGCTAAAGCTAAACCAGCTCAAGTTTCGGAAGAAGAAACTCCGATTTGGCCGGAGCCGTATCCA
TGAGTGGGGTCTGTTTGCCATGGAACCCATTGCCGCTGATGAGATGGTGATCGAATATGTTGGCCAGAAC
ATCCGCCAGATGGTAGCGGACATGCGGGAAAAGCGCTACGTGCAGGAGGGCATTGGCAGCAGCTACCTTT
TCCGGGTGGATCACGACACCATCATTGATGCCACCAAGTGTGGCAACCTCGCCAGGTTCATCAACCACTG
CTGCACGCCCAACTGCTATGCGAAGGTGATCACCATTGAGTCTCAGAAAAAGATTGTCATCTACTCGAAG
CAGCCCATCGGCGTGGATGAGGAGATCACCTACGACTACAAGTTCCCACTAGAAGACAACAAGATTCCGT
GTCTGTGCGGCACTGAAAGTTGCCGTGGCTCCCTCAAC

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG215352 representing NM_178029
Red=Cloning site Green=Tags(s)

MDQEGGGDGQKAPSFQWRNYKLIVDPALDPALRRPSQKVYRYDGVHFSVSDSKYTPVEDLQDPRCHVRSK
ARDFSLPVPKFKLDEFYIGQIPLKEVTFARLNDNVRETFLKDMCRKYGEVEEVEILLHPRTRKHLGLARV
LFTSTRGAKETVKNLHLTSVMGNIIHAQLDIKGQQRMKYYELIVNGSYTPQTVPTGGKALSEKFQGSGAA
AETTEARRRSSSDTAAYPAGTTVGGTPGNGTPCSQDTNFSSSRQDTPSSFGQFTPQSSQGTPYTSRGSTP
YSQDSAYSSSTTSTSFKPRRSENSYQDSFSRRHFSTSSAPATTATATSATAAATAASSSSSSSSSSSSSS
SSSSSASQFRGSDSSYPAYYESWNRYQRHTSYPPRRATREDPSGASFAENTAERFPPSYTSYLAPEPNRS
TDQDYRPPASEAPPPEPPEPGGGGGGSGGGGGGGGGGGGGAPSPEREEARTPPRPASPARSGSPAPETTN
ESVPFAQHSSLDSRIEMLLKEQRSKFSFLASDTEEEEENSSAGPGARDAGAEVPSGAGHGPCTPPPAPAN
FEDVAPTGSGEPGAARESPKANGQNQASPCSSGEDMEISDDDRGGSPPPAPTPPQQPPPPPPPPPPPPPP
YLASLPLGYPPHQPAYLLPPRPDGPPPPEYPPPPPPPPPHIYDFVNSLELMDRLGAQWGGMPMSFQMQTQ
MLTRLHQLRQGKGLTAASAGPPGGAFGEAFLPFPPPQEAAYGLPYALYTQGQEGRGSYSREAYHLPLPMA
AEPLPSSSVSGEEARLPHREEAEIAESKVLPSAGTVGRVLATLVQEMKSIMQRDLNRKMVENVAFGAFDQ
WWESKEEKAKPFQNAAKQQAKEEDKEKMKLKEPGMLSLVDWAKSGGITGIEAFAFGSGLRGALRLPSFKV
KRKEPSEISEASEEKRPRPSTPAEEDEDDPEREKEAGEPGRPGTKPPKRDEERGKTQGKHRKSFTLDSEG
EEASQESSSEKDEDDDDEDEEDEEQEEAVDATKKEAEASDGEDEDSDSSSQCSLYADSDGENGSTSDSES
GSSSSSSSSSSSSSSSSSSESSSEEEEQSAVIPSASPPREVPEPLPAPDEKPETDGLVDSPVMPLSEKET
LPTQPAGPAEEPPPSVPQPPAEPPAGPPDAAPRLDERPSSPIPLLPPPKKRRKTVSFSAAEEAPVPEPST
AAPLQAKSSGPVSRKVPRVVERTIRNLPLDHASLVKSWPEEVARGGRNRAGGRVRSTEEEEATESGTEVD
LAVLADLALTPARRGLATLPTGDDSEATETSDEAERPSPLLSHILLEHNYALAIKPPPTTPAPRPLEPAP
ALAALFSSPADEVLEAPEVVVAEAEEPKQQLQQQHPEQEGEEEEEDEEEESESSESSSSSSSDEEGAIRR
RSLRSHTRRRRPPLPPPPPPPPSFEPRSEFEQMTILYDIWNSGLDLEDMSYLRLTYERLLQQTSGADWLN
DTHWVQHTITNLSTPKRKRRPQDGPREHQTGSARSEGYYPISKKEKDKYLDVCPVSARQLEGGDTQGTNR
VLSERRSEQRRLLSAIGTSAIMDSDLLKLNQLKFRKKKLRFGRSRIHEWGLFAMEPIAADEMVIEYVGQN
IRQMVADMREKRYVQEGIGSSYLFRVDHDTIIDATKCGNLARFINHCCTPNCYAKVITIESQKKIVIYSK
QPIGVDEEITYDYKFPLEDNKIPCLCGTESCRGSLN

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_178029
ORF Size 5148 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_178029.3, NP_821172.2
RefSeq Size 5934 bp
RefSeq ORF 5151 bp
Locus ID 233904
UniProt ID E9PYH6
Cytogenetics 7 F3
Summary Histone methyltransferase that specifically methylates 'Lys-4' of histone H3, when part of the SET1 histone methyltransferase (HMT) complex, but not if the neighboring 'Lys-9' residue is already methylated. H3 'Lys-4' methylation represents a specific tag for epigenetic transcriptional activation. The non-overlapping localization with SETD1B suggests that SETD1A and SETD1B make non-redundant contributions to the epigenetic control of chromatin structure and gene expression.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_178029" in other vectors (2)
SKU Description Size Price
MC224870 Setd1a (untagged) - Mouse SET domain containing 1A (Setd1a), (10ug) 10 ug
$1,587.00
MR215352 Setd1a (Myc-DDK-tagged) - Mouse SET domain containing 1A (Setd1a). Note: ORF is codon optimized 10 ug
$1,442.00

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