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Rapgef6 (NM_175258) Mouse Tagged ORF Clone

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SKU
MG215350
Rapgef6 (tGFP-tagged) - Mouse Rap guanine nucleotide exchange factor (GEF) 6 (Rapgef6), (10ug)
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ORF Plasmid:
tGFP DDK
  $1,569.00
3 Weeks*
Bulk Requests & Clone Modifications

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Other products for "Rapgef6 or NM_175258"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Rapgef6
Synonyms A530068K01; AI844632; BB085664; C030018K18Rik; mKIAA4052; PDZ-GEF2; Pdzgef2; RA-GEF-2
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG215350 representing NM_175258
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAACTCGCCCGTGGACCCCGGCGCTCGGCAGGCACTGAGGAAGAAGCCGCCCGAGCGGACTCCTGAGG
ACTTAAATATTATTTATTCTTATCTGCATGGGATGGAAATACTATCAAACCTCAGGGAGCATCAGCTTAG
GTTAATGTCTACAAGAGCACGCTATGAGAGATACAGTGGCAATCAAATGCTTTTTTGTTCAGAAACTATT
GCCAGATGTTGGTATATCCTGCTTTCTGGATCTGTACTTGTAAAAGATTCTATGGTCCTTCCTCCTTGCA
GTTTTGGTAAGCAGTTTGGAGGAAAAAGAGGATGTGATTGTCTTGTGTTAGAACCTTCAGAAATGATTGT
GGTAGAGAATTCCAAAGATAATGAAGACAGTATTTTACAAAGAGAAATTCCTGCCAGACAGTCTCGACGA
AGGTTTCGGAAAATTAACTATAAAGGAGAACGCCAAACCATAATAGATGGAGTGGATATTAATAACTACC
TTTCGCTTCCAGCTGATCTTACCAAGATGCACCTCACAGACAACCCTCATCCACAGGTGACGCATGTGTC
TTCTAGTCAGTCTGGCTGTAGCATTGCCAGTGACTCTGGGAGCAGCAGTTTATCCGATATCTATCAGGCT
ACAGAGAGTGAAGTAGGAGATGTAGATTTAACACGCCTTCCAGAAGGACCTGTTGATTCTGAGGATGAAG
AGGAGGAGGAAGAAGAGATTGATAGAACAGATCCACTGCAGGGTCGAGATCTTGTTCGGGAGTGTCTTGA
AAAGGAACCCGCAGACAAAACTGATGATGATGTTGAACAGTTACTTGAGTTCATGCACCAGCTGCCTGCC
TTTGCGAACATGACCATGTCTGTAAGGAGAGAACTCTGCTCCGTCATGGTTTTTGAAGTAGTAGAGCAGG
CTGGGGCTGTTATTCTTGAAGATGGGCAAGAGCTTGACTCATGGTATGTCATTTTAAATGGCACTGTAGA
AATTAGTCATCCAGATGGGAAAATAGAAAATCTCTTCATGGGAAATAGTTTTGGGATTGTCCCCACGCTG
GATAAGCAGCACATGCATGGAGCTGTCAGGACCAAAGTCGATGATTGTCAGTTTGTCTGCATAGCTCAAC
AAGACTATTGGAGAATTTTAAATCATGTGGAAAAAAATACTCACAAAGTTGAGGAAGAGGGAGAGATTGT
CATGGTACATGAGCACCGTGAGCTAGACCGGAGCGGAACCAGGAAAGGACACATTGTAATCAAGGCAACA
CCTGAGCGTCTCATCATGCATTTGATAGAAGAACATTCTATTGTGGACCCAACATACATAGAAGATTTCC
TCTTAACTTACAGGACATTTCTTGAAACTCCTTTGGATGTTGGGATCAAGCTTTTGGAATGGTTTAAGAT
TGACAACCTAAGGGATAAGGTGACACGGATTGTACTCTTATGGGTAAATAATCATTTTAATGATTTTGAA
GGTGATCCTGCTATGACTCAATTTCTAGAAGAATTTGAAAGAAATTTGGAAGATACAAAAATGAATGGTC
ACCTCAGGTTATTGAATATTGCCTGTGCTGCAAAGGCTAAGTGGAGGCAAGTTGTACTGCAGAAGGCGTC
CCGTGAGTCCCCGCTGCACTTCTGCCTTACTGGGGGCAGTGAGAAGGGATTTGGAGTCTTTGTTGAAGAA
GTAGAATCTGGTAGCAAAGCTGCTGACGCAGGACTGAAAAGGGGTGATCAGGTAATGGAAGTCAATGGAC
AAAACTTTGAGAATATTACACTTGCTAAAGCCCTTGAAATTTTGAGAAATAATACTCATCTTGCACTTAC
TGTGAAGACCAACATTTTTGTGTTCAAAGAATTGCTCAGTAGGACTGAACAAGAGAAATCTGGTGTTCCT
CATATTCCCAAAATTGCTGAAAAAAAAAGTAATCGCCATTCAATTCAAGATGTCCCAGGGGATATGGAGC
AGGCACCACAGGAGAAAGGAAATAAGAAAATTAAAGCAAATACCGTTTCAGGTGGAAGAAACAAAATCAG
GAAAATTTTGGATAAAACACGATTTAGTATCTTGCCTCCAAAACTATTTAGTGACGGAGGCCTGAGTCAA
TCACAAGATGACAGCATTGTGGGAACCAGGCACTGTAGGCACAGCCTGGCTATAATGCCAATTCCCGGAA
CACTTTCATCCAGCAGCCCAGATCTCTTACAGCCTACCACTAGCATGTTGGACTTTTCCAATCCTTCAGA
TATTCCTGATCAAGTTATAAGAGTTTTCAAAGCAGATCAACAAAGTTGCTACATTATCATCAGTAAGGAT
ACCACAGCTAAAGAAGTGGTCTGCCAAGCTGTACAGGAATTCGGTTTGACTGGTGCATCTGACACATACT
CCCTCTGTGAAGTGTCTGTTACTCCTGAGGGTGTTATAAAGCAGAGAAGACTTCCGGACCAGTTCTCCAA
ATTAGCTGATAGAATTCAACTCAATGGAAGGTATTACCTAAAAAACAACATGGAAACAGAAACATTGTGT
TCTGATGAAGATGCTCAGGAACTACTTAAGGAAAGCCAGCTCTCCATGCTGCAGCTCAGTACCATCGAGG
TCGCCACCCAGCTCTCCATGAGAGACTTTGATTTATTTCGTAACATTGAGCCTACTGAATACATTGATGA
TCTTTTTAAATTAGATTCCAAAACAGGAAATACTCACTTGAAACAATTTGAGGATATTGTAAACCAAGAG
ACATTCTGGGTTGCCTCAGAGATTTTAAGTGAGTCAAATCAGCTGAAAAGAATGAAGATTATTAAACATT
TTATCAAAATTGCTCTTCATTGCCGAGAATGCAAGAATTTCAATTCCATGTTTGCAATAATAAGTGGCTT
GAACCTGGCACCGGTAGCACGACTCAGAGGCACTTGGGAAAAGTTGCCAAGCAAATATGAGAAACATCTT
CAAGATCTACAAGACCTTTTTGATCCATCTAGAAATATGGCAAAATACAGAAATATTCTTAGTAGTCAAA
GCATGCAGCCTCCAATTATTCCACTCTTCCCTGTTGTCAAGAAAGATATGACATTTCTCCATGAAGGAAA
TGACTCCAAAGTAGATGGCTTAGTAAACTTCGAGAAGCTAAGAATGATTGCCAAGGAAATCCGTCATATT
ATTCGAATGACTTCTGCCAACATGGACCCTGCCATGATGTTCCGACAGAGGTCATTGAGTCAAGGCAGCA
CAAATTCTAACATGCTAGATGTCCAGGGAGGTGCTCACAAAAAAAGAGCACGCCGCAGCTCACTGCTGAA
TGCCAAGAAACTGTATGAAGATGCCCAAATGGCAAGGAAAGTGAAACAGTATCTTTCTAGTCTGGACATA
GACACAGATGAGGAGAAGTTCCAGATGATGTCACTGCAGTGGGAACCTGCATATGGTACCTTGACAAAGA
ACTTAACTGAAAAAAGGTCAGCCAAATCATCTGAGATGTCTCCTGTACCATTGAGGTCAGTTGGCCAAAC
AGCTAAAGTCCACTTGCATCAGCCACACAGAGTGAGCCAGGTGCTTCAGGTGCCAGCAGTTAACTTGCAC
CCCATCCGGAAGAAAGGACAAGCCAAAGACCATGTGCTGAGTACAAGTTTACCTCAGAAAGGTTTAGGAC
CAACTGAGGAAGTGAGTGTTAAGAAACACACAGAAGACACTATTTCTGTAGCGTCCTCTCTGCACTCTAG
CCCTCCTGCATCTCCTCAGAACTCCCCACGCAAAGGTTATACACTCACACCATCAAGTAAATGTGACAAC
TTGTCTGACTCCAGCCACAGTGAAATCTCCTCTCGGTCCAGCATTGTGAGCAATGGTTCTGTTGACTCCA
TGTCTGCAGCTGGGCAGGATGAACGCTGTTCCTCCCACAGTCTGGCAGTTCCTGAGCCCACAGGGGCATT
GGAAAAGACAGATCACCCTTCCGGGATCTCAGACCACAGTCAGCTTGCTCACGGGTGGATGCTGTCAAAG
CCGTGTCTTATCAAGGGTGTAGCTGTCTCATCTTCTCTGAGCAGCGAAGAGATGTCTCATGAGCATGTTG
TGCTGGAAGCAGCTGACAGTGGGCGGGGCAGTTGGACTTCCTGTTCCAGCAGCTCCCATGACAACTTCCA
AAGCCTTCAAAATCAAAAAAGCTGGGACTTCCTGAATTCTTACAGACACATGCATTTAGATGACCCTATT
GCAGAAGTTGAGCCCACTGACTGTGAGCCCTGTGCCTGTCCTAAAGGCTGCTCAAGAACTTGTGGCCAGT
GCAAAGGCAGCCTAGAGACGAACCAGCTGAGGCAGAGTTGGGCCTCCTCCAGCTCACTCTCGGACACATG
TGAACCAAACTACGGGACAGTTAAGCGGAGGGTGTTGGAAAGTGCACCAGCAGAGGCACCTGATGGCTTG
GAGCCCAGGGATACCACTGACCCTGTTTATAAAACTGTCACTTCCAGTACAGACAAGGGCTTGATTGTGT
ACTGTGTCACCTCACCCAAGAAAGGTGATAGGTATAGGGAGCCACCGCCCACTCCTCCAGGATATCTGGG
AATTTCTTTAGCGGACCTAAAGGAAGGACCCCACCCGCACCTAAAACCTCCAGACTATAGTGTGGCAGTA
CAGAGATCAAAGATGATGCTTAACAGCCTGTCTAGACTGCCGCCAGCTCCTCCCAGTAGCCACACCTCGG
CCTGGGTTCCCTCGAAGATTGGATCCCAGCCTCAAAGGCATAGCCATCCCAAGCTAGCAGATGTGGCTGA
TGCAGATAGCGAAGCAGATGAAAACGAGCAGGTGTCAGCAGTC

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG215350 representing NM_175258
Red=Cloning site Green=Tags(s)

MNSPVDPGARQALRKKPPERTPEDLNIIYSYLHGMEILSNLREHQLRLMSTRARYERYSGNQMLFCSETI
ARCWYILLSGSVLVKDSMVLPPCSFGKQFGGKRGCDCLVLEPSEMIVVENSKDNEDSILQREIPARQSRR
RFRKINYKGERQTIIDGVDINNYLSLPADLTKMHLTDNPHPQVTHVSSSQSGCSIASDSGSSSLSDIYQA
TESEVGDVDLTRLPEGPVDSEDEEEEEEEIDRTDPLQGRDLVRECLEKEPADKTDDDVEQLLEFMHQLPA
FANMTMSVRRELCSVMVFEVVEQAGAVILEDGQELDSWYVILNGTVEISHPDGKIENLFMGNSFGIVPTL
DKQHMHGAVRTKVDDCQFVCIAQQDYWRILNHVEKNTHKVEEEGEIVMVHEHRELDRSGTRKGHIVIKAT
PERLIMHLIEEHSIVDPTYIEDFLLTYRTFLETPLDVGIKLLEWFKIDNLRDKVTRIVLLWVNNHFNDFE
GDPAMTQFLEEFERNLEDTKMNGHLRLLNIACAAKAKWRQVVLQKASRESPLHFCLTGGSEKGFGVFVEE
VESGSKAADAGLKRGDQVMEVNGQNFENITLAKALEILRNNTHLALTVKTNIFVFKELLSRTEQEKSGVP
HIPKIAEKKSNRHSIQDVPGDMEQAPQEKGNKKIKANTVSGGRNKIRKILDKTRFSILPPKLFSDGGLSQ
SQDDSIVGTRHCRHSLAIMPIPGTLSSSSPDLLQPTTSMLDFSNPSDIPDQVIRVFKADQQSCYIIISKD
TTAKEVVCQAVQEFGLTGASDTYSLCEVSVTPEGVIKQRRLPDQFSKLADRIQLNGRYYLKNNMETETLC
SDEDAQELLKESQLSMLQLSTIEVATQLSMRDFDLFRNIEPTEYIDDLFKLDSKTGNTHLKQFEDIVNQE
TFWVASEILSESNQLKRMKIIKHFIKIALHCRECKNFNSMFAIISGLNLAPVARLRGTWEKLPSKYEKHL
QDLQDLFDPSRNMAKYRNILSSQSMQPPIIPLFPVVKKDMTFLHEGNDSKVDGLVNFEKLRMIAKEIRHI
IRMTSANMDPAMMFRQRSLSQGSTNSNMLDVQGGAHKKRARRSSLLNAKKLYEDAQMARKVKQYLSSLDI
DTDEEKFQMMSLQWEPAYGTLTKNLTEKRSAKSSEMSPVPLRSVGQTAKVHLHQPHRVSQVLQVPAVNLH
PIRKKGQAKDHVLSTSLPQKGLGPTEEVSVKKHTEDTISVASSLHSSPPASPQNSPRKGYTLTPSSKCDN
LSDSSHSEISSRSSIVSNGSVDSMSAAGQDERCSSHSLAVPEPTGALEKTDHPSGISDHSQLAHGWMLSK
PCLIKGVAVSSSLSSEEMSHEHVVLEAADSGRGSWTSCSSSSHDNFQSLQNQKSWDFLNSYRHMHLDDPI
AEVEPTDCEPCACPKGCSRTCGQCKGSLETNQLRQSWASSSSLSDTCEPNYGTVKRRVLESAPAEAPDGL
EPRDTTDPVYKTVTSSTDKGLIVYCVTSPKKGDRYREPPPTPPGYLGISLADLKEGPHPHLKPPDYSVAV
QRSKMMLNSLSRLPPAPPSSHTSAWVPSKIGSQPQRHSHPKLADVADADSEADENEQVSAV

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_175258
ORF Size 4803 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_175258.4, NP_780467.2
RefSeq Size 8212 bp
RefSeq ORF 4806 bp
Locus ID 192786
Cytogenetics 11 B1.3
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Documents
Resources
"NM_175258" in other vectors (4)
SKU Description Size Price
MC224739 Rapgef6 (untagged) - Mouse Rap guanine nucleotide exchange factor (GEF) 6 (Rapgef6), (10ug) 10 ug
$1,507.00
MR215350 Rapgef6 (Myc-DDK-tagged) - Mouse Rap guanine nucleotide exchange factor (GEF) 6 (Rapgef6) 10 ug
$589.00 $1,369.00
MR215350L3 Lenti ORF clone of Rapgef6 (Myc-DDK-tagged) - Mouse Rap guanine nucleotide exchange factor (GEF) 6 (Rapgef6) 10 ug
$1,669.00
MR215350L4 Lenti ORF clone of Rapgef6 (mGFP-tagged) - Mouse Rap guanine nucleotide exchange factor (GEF) 6 (Rapgef6) 10 ug
$1,669.00

file-alt Citations

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