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Rtf1 (NM_030112) Mouse Tagged ORF Clone

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SKU
MG215339
Rtf1 (tGFP-tagged) - Mouse Rtf1 Paf1/RNA polymerase II complex component homolog (S. cerevisiae) (Rtf1), (10ug)
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ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $855.00
3 Weeks*
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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Rtf1
Synonyms 2900005O08Rik; 6530416A09Rik; AI853581; AU043144; AW553985; cbp82; Gtl7
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG215339 representing NM_030112
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCGCGGTCGCCTTTGTGTGGGTCGAGCGGCGGCGGTGGCGGCGGCGGTGGCGGCGGCGGCAGTGGCGG
TCCCACTGGCAGGCGGGCAAGAGGGGAGTCAGGGCGGCGTCCGGCGTGGAAGCCGGGGGACCACCATGGT
AAAGAAGCGGAAAGGCCGTGTCGTGATCGACTCGGATACGGAAGACAGCGGCAGTGACGAGAACCTGGAT
CAGGAGCTCTTGTCCCTGGCGAAACGGAAGCGCAGTGACTCCGAGGAGAAGGAGCCTCCTGTGAGTCAGC
CTGCAGCCTCATCAGATTCAGAGACCTCCGACAGTGATGATGAGTGGACATTTGGGAGCAATAAAAATAA
GAAGAAAGGAAAAACCAGAAAAGTAGAGAAGAAAGGAGCCATGAAGAAGCAAGCCAACAAAGCTGCCTCC
TCGGGCAGCTCAGACAGAGACAGCTCAGCTGAGAGCTCTGCCCCTGAGGAAGGTGAAGTGTCAGATTCTG
AGAGCAGCAGCTCCTCTTCCAGTTCAGATTCAGACTCCTCGTCAGAAGATGAAGAATTCCATGATGGCTA
TGGAGAGGATCTTATGGGAGATGAGGAAGATAGGGCCCGACTGGAGCAGATGACGGAGAAGGAGAGGGAG
CAGGAGCTCTTCAACCGCATAGAGAAGAGAGAGGTGTTGAAAAGAAGATTTGAAATTAAGAAAAAACTAA
AAACAGCCAAAAAGAAAGAGAAGAAAGAAAAAAAGAAAAAGCAGGAAGAAGAACAAGAAAAGAAGAAGCT
GACACAGATTCAGGAATCTCAGGTTACTTCCCACAATAAAGAACGGCGTTCCAAGCGGGATGAAAAACTA
GATAAGAAATCTCAAGCCATGGAGGAGCTAAAAGCAGAGCGAGAAAAACGGAAGAACAGAACAGCTGAGC
TCCTCGCCAAAAAACAGCCATTGAAAACCAGTGAGGTCTACTCTGACGATGAGGAGGAGGAAGATGATGA
CAAGTCCAGTGAGAAATCGGACCGCTCATCCCGGACATCATCATCTGATGAAGAGGAAGAGAAAGAAGAA
ATCCCTCCAAAATCACAGCCAGTCTCATTACCCGAGGAACTGAATCGGGTTCGGTTATCACGGCATAAGC
TAGAGCGCTGGTGTCACATGCCCTTCTTTGCTAAAACTGTCACAGGATGTTTTGTACGGATTGGCATTGG
AAACCACAATAGCAAGCCAGTTTACCGGGTGGCTGAGATCACGGGTGTTGTGGAAACTGCCAAAGTTTAC
CAGCTGGGTGGCACCAGAACAAATAAAGGGCTCCAGCTACGGCATGGCAATGACCAGCGAGTGTTCCGCC
TAGAGTTTGTGTCCAACCAAGAATTCACTGAAAGTGAATTCATGAAGTGGAAAGAAGCGATGTTTTCTGC
TGGCATGCAGTTGCCCACACTAGATGAAATCAATAAAAAGGAATTATCTATTAAGGAAGCTCTCAATTAT
AAATTCAATGATCAGGACATTGAGGAGATTGTAAAAGAGAAGGAAAGGTTCAGAAAAGCCCCACCAAACT
ATGCTATGAAGAAGACTCAGCTGCTAAAGGAGAAGGCTATGGCTGAGGACCTGGGAGATCAGGACAAAGC
CAAACAAATCCAAGACCAGCTAAATGAGCTAGAGGAGCGAGCAGAGGCCCTGGACCGCCAGCGGACCAAG
AACATATCTGCTATCAGCTACATCAACCAGCGGAATCGGGAGTGGAATATTGTGGAGTCTGAGAAGGCTC
TGGTGGCTGAAAGTCACAACATGAGAAACCAACAGATGGACCCCTTCACCAGGCGGCAGTGCAAACCCAC
CATCGTCTCTAATTCCAGGGATCCAGCTGTTCAGGCTGCCATTTTGGCCCAGCTCAATGCAAAATACGGT
TCTGGGGTATTACCAGATGCTCCAAAGGAAATGAGCAAGGGTCAAGGAAAGGATAAAGATTTGAATTCTA
AGACAGCCAGTGACCTCTCGGAAGACCTGTTCAAAGTGCATGACTTTGACGTGAAGATTGACTTACAGGT
TCCAAGCTCAGAGTCAAAAGCTTTGGCCATTACCTCCAAAGCCCCACCAGCCAAGGACGGAGCTCCGAGG
AGATCTCTGAACTTAGAAGACTACAAAAAACGACGGGGGCTTATT

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG215339 representing NM_030112
Red=Cloning site Green=Tags(s)

MRGRLCVGRAAAVAAAVAAAAVAVPLAGGQEGSQGGVRRGSRGTTMVKKRKGRVVIDSDTEDSGSDENLD
QELLSLAKRKRSDSEEKEPPVSQPAASSDSETSDSDDEWTFGSNKNKKKGKTRKVEKKGAMKKQANKAAS
SGSSDRDSSAESSAPEEGEVSDSESSSSSSSSDSDSSSEDEEFHDGYGEDLMGDEEDRARLEQMTEKERE
QELFNRIEKREVLKRRFEIKKKLKTAKKKEKKEKKKKQEEEQEKKKLTQIQESQVTSHNKERRSKRDEKL
DKKSQAMEELKAEREKRKNRTAELLAKKQPLKTSEVYSDDEEEEDDDKSSEKSDRSSRTSSSDEEEEKEE
IPPKSQPVSLPEELNRVRLSRHKLERWCHMPFFAKTVTGCFVRIGIGNHNSKPVYRVAEITGVVETAKVY
QLGGTRTNKGLQLRHGNDQRVFRLEFVSNQEFTESEFMKWKEAMFSAGMQLPTLDEINKKELSIKEALNY
KFNDQDIEEIVKEKERFRKAPPNYAMKKTQLLKEKAMAEDLGDQDKAKQIQDQLNELEERAEALDRQRTK
NISAISYINQRNREWNIVESEKALVAESHNMRNQQMDPFTRRQCKPTIVSNSRDPAVQAAILAQLNAKYG
SGVLPDAPKEMSKGQGKDKDLNSKTASDLSEDLFKVHDFDVKIDLQVPSSESKALAITSKAPPAKDGAPR
RSLNLEDYKKRRGLI

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_030112
ORF Size 2145 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_030112.2, NP_084388.2
RefSeq Size 4680 bp
RefSeq ORF 2148 bp
Locus ID 76246
UniProt ID A2AQ19
Cytogenetics 2 E5
Summary Component of the PAF1 complex (PAF1C) which has multiple functions during transcription by RNA polymerase II and is implicated in regulation of development and maintenance of embryonic stem cell pluripotency. PAF1C associates with RNA polymerase II through interaction with POLR2A CTD non-phosphorylated and 'Ser-2'- and 'Ser-5'-phosphorylated forms and is involved in transcriptional elongation, acting both indepentently and synergistically with TCEA1 and in cooperation with the DSIF complex and HTATSF1. PAF1C is required for transcription of Hox and Wnt target genes. PAF1C is involved in hematopoiesis and stimulates transcriptional activity of KMT2A/MLL1. PAF1C is involved in histone modifications such as ubiquitination of histone H2B and methylation on histone H3 'Lys-4' (H3K4me3). PAF1C recruits the RNF20/40 E3 ubiquitin-protein ligase complex and the E2 enzyme UBE2A or UBE2B to chromatin which mediate monoubiquitination of 'Lys-120' of histone H2B (H2BK120ub1); UB2A/B-mediated H2B ubiquitination is proposed to be coupled to transcription. PAF1C is involved in mRNA 3' end formation probably through association with cleavage and poly(A) factors. Binds single-stranded DNA (By similarity). Required for maximal induction of heat-shock genes. Required for the trimethylation of histone H3 'Lys-4' (H3K4me3) on genes involved in stem cell pluripotency; this function is synergistic with CXXC1 indicative for an involvement of a SET1 complex.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_030112" in other vectors (4)
SKU Description Size Price
MC221020 Rtf1 (untagged) - Mouse Rtf1, Paf1/RNA polymerase II complex component, homolog (S. cerevisiae) (Rtf1), (10ug) 10 ug
$721.00
MR215339 Rtf1 (Myc-DDK-tagged) - Mouse Rtf1, Paf1/RNA polymerase II complex component, homolog (S. cerevisiae) (Rtf1) 10 ug
$589.00 $655.00
MR215339L3 Lenti ORF clone of Rtf1 (Myc-DDK-tagged) - Mouse Rtf1, Paf1/RNA polymerase II complex component, homolog (S. cerevisiae) (Rtf1) 10 ug
$955.00
MR215339L4 Lenti ORF clone of Rtf1 (mGFP-tagged) - Mouse Rtf1, Paf1/RNA polymerase II complex component, homolog (S. cerevisiae) (Rtf1) 10 ug
$955.00

file-alt Citations

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