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Pspn (NM_008954) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG215304
Pspn (tGFP-tagged) - Mouse persephin (Pspn), (10ug)
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ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $350.00
3 Weeks*
Bulk Requests & Clone Modifications

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Other products for "Pspn or NM_008954"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Pspn
Synonyms PSP
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG215304 representing NM_008954
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCTGCAGGAAGACTTCGGATCCTGTGTCTGCTGCTCCTGTCCTTGCACCCGAGCCTCGGCTGGGTCC
TTGATCTTCAAGAGGCTTCTGTGGCAGATAAGCTCTCATTTGGGAAGATGGCAGAGACTAGAGGGACCTG
GACGCCCCATCAGGGTAACAACCATGTCCGTCTTCCAAGAGCCTTGGCTGGTTCATGCCGACTGTGGAGC
CTGACCCTACCAGTGGCTGAGCTGGGCCTGGGCTATGCCTCGGAGGAGAAGGTCATCTTCCGATACTGTG
CTGGCAGCTGTCCCCAAGAGGCCCATACCCAGCACAGTCTGGTACTGGCCCGGCTTCGAGGGCGGGGTCG
AGCCCATGGCCGACCCTGCTGCCAGCCCACCAGCTATGCTGATGTGACCTTCCTTGATGATCAGCACCAT
TGGCAGCAGCTGCCTCAGCTCTCAGCTGCAGCTTGTGGCTGTGGTGGC

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG215304 representing NM_008954
Red=Cloning site Green=Tags(s)

MAAGRLRILCLLLLSLHPSLGWVLDLQEASVADKLSFGKMAETRGTWTPHQGNNHVRLPRALAGSCRLWS
LTLPVAELGLGYASEEKVIFRYCAGSCPQEAHTQHSLVLARLRGRGRAHGRPCCQPTSYADVTFLDDQHH
WQQLPQLSAAACGCGG

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_008954
ORF Size 468 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_008954.3
RefSeq Size 474 bp
RefSeq ORF 471 bp
Locus ID 19197
Cytogenetics 17 D
Summary This gene encodes a secreted ligand of the GDNF (glial cell line-derived neurotrophic factor) subfamily and TGF-beta (transforming growth factor-beta) superfamily of proteins. The encoded preproprotein is proteolytically processed to generate the mature protein. This protein signals through the RET receptor tyrosine kinase and a GPI-linked coreceptor, and promotes survival of neuronal populations. This protein may play a role in cell death, and nervous system development and function. Mice lacking a functional copy of this gene exhibit hypersensitivity to cerebral ischemia. provided by RefSeq, Aug 2016
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Documents
Resources
"NM_008954" in other vectors (4)
SKU Description Size Price
MC209248 Pspn (untagged) - Mouse persephin (Pspn), (10ug) 10 ug
$165.00
MR215304 Pspn (Myc-DDK-tagged) - Mouse persephin (Pspn) 10 ug
$289.00
MR215304L3 Lenti ORF clone of Pspn (Myc-DDK-tagged) - Mouse persephin (Pspn) 10 ug
$450.00
MR215304L4 Lenti ORF clone of Pspn (mGFP-tagged) - Mouse persephin (Pspn) 10 ug
$450.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.