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Epc1 (NM_027497) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG215235
Epc1 (tGFP-tagged) - Mouse enhancer of polycomb homolog 1 (Drosophila) (Epc1) transcript variant 2, (10ug)
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ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $945.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Epc1
Synonyms 2400007E14Rik; 5730566F07Rik; A930032N02Rik; mir-1893
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG215235 representing NM_027497
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAGTAAACTGTCGTTTCGGGCGCGGGCGCTGGACGCCTCGAAGCCGCTGCCGGTCTTCCGCTGTGAGG
ATCTGCCCGACCTGCACGAATACGCCTCGATAAACCGGGCCGTGCCGCAGATGCCCACCGGGATGGAGAA
GGAAGAGGAGTCGGAACACCATCTTCAGCGGGCTATTTCTGCACAGCAGGTGTACGGCGAGAAGCGGGAT
AACATGGTTATTCCAGTTCCTGAAGCAGAGAGTAACATCGCTTACTATGAGTCCATATACCCTGGGGAGT
TCAGGATGCCAAAGCAGCTCATTCACATACAGCCTTTTAGCTTGGACGCTGAACAGCCTGATTACGATCT
GGATTCTGAAGATGAAGTATTTGTGAATAAACTGAAGAAGAAAATGGACATCTGCCCATTGCAGTTTGAG
GAGATGATTGACCGGCTAGAAAAAGGCAGCGGTCAGCAGCCAGTCAGTCTGCAGGAAGCCAAACTACTTC
TAAAAGAAGATGATGAGTTAATTAGAGAAGTTTATGAATACTGGATTAAAAAGAGGAAAACCTGCCGGGG
GTCATCACTTATCCCGTTGGTAAAACAGGAAAAGCGAGATGGTTCTAGCACAAATGATCCTTACGTGGCT
TTTAGAAGACGAACTGAGAAAATGCAAACTCGGAAAAACCGTAAGAATGATGAAGCCTCATATGAGAAAA
TGCTCAAGCTGCGCCGCGATCTCAGCCGGGCTGTCACCATCCTGGAAATGATCAAGAGACGGGAGAAGAG
TAAACGGGAGCTGCTGCACCTGACCCTGGAAATCATGGAGAAGAGGTATAATTTAGGTGACTATAGTGGA
GAGATCATGTCTGAGGTCATGGCACAGAGACAGCCAGTGAAGCCTACTTATGCCATCCCCATCATCCCGA
TTACTAACAGCAGCCAGTTCAAACACCAGGATGCCACGGACTCGAAGGAGTTCAAAGTTAACAAGCAAGA
TAAGGCTGATCTTATCCGGCCGAAGCGCAAATATGAAAAGAAGCCCAAAGTCTTACCCCCATCTGCTGCC
GCCCCTCAGCAGCAGAGTCCTGCTGCACTGCCAGGCTTCAGTGCTAAAGACTTAAACCAGTACGACTTCC
CCAGCTCAGATGAAGAGCCTCTGTCACAGGTTTTGTCTGGCTCTTCGGAAGCTGAGGAAGAGAATGACCC
TGATGGCCCTTTTGCTTTCCGTAGGAAAGCAGGCTGTCAGTACTATGCTCCTCATTTAGACCAAACTGGC
AACTGGCCCTGGACTAGCCCTAAAGATGGAGGCCTGGGGGATGTGCGATATAGATATTGCTTAACTACTC
TCACCGTACCCCAGAGGTGTCTTGGATTTGCACGAAGACGGGTCGGACGTGGTGGAAGGGTCGTGCTGGA
CAGAGCGCATTCAGACTATGACAGTATGTTTCACCACCTGGATTTGGACATGCTTTCCTCACCACAACCT
TCTCCAGTCAATCAGTTTGCCAATACCTCAGAACCCAATACCTCGGACAGATCTTCCTCTAAAGACCTCA
GTCAGATACTAGTCGATATCAAATCATGTAGATGGCGGCACTTTAGGCCTCGGACACCATCCCTGCCTGA
CAGTGACAGTGGTGAACTCTCCAGTAGAAAGTTACACAGGAGTATCAGTCGAGCAGGAGCAGCACAGCCT
GGGGCCCACACGTGCAGCACCTCCACCCAGAACAGAAGTAGCAGTGGCTCAGCACACTGTGCATTCACAG
CCGAACAGTACCAGCAGCACCAGCAGCAGCTGGCACTCATGCAGCAGCAGCAGCTTGCGCAGACTCAGCA
GCAGCAGCAAGCAAATAGTAGTTCCTCTGCCGCCGCGCAACAGGGTTTTGTTTCTAAGACGTTGGACTCT
GCTAGTGCACAGTTTGCTGCTTCTGCTTTGATGACGTCGGAACAGCTGCTGGGGTTCAAGGTGAAGGATG
ATGTGGTGCTCGGGCTCGGGGTGAATGGCGTCCTTCCTGCCTCAGGAGTATACAAGGGCTTACACCTCAG
TAGCACTACACCAACGGCACTTGTACATACGAGTCCTTCGACAGCAGGTTCAACTTTGTTACAGCCTTCA
AATATTACACAGACTTCGGGTTCCCACAGCTCACTGAGTCACCAAGTAACTGCTGCCAGCTCTGCAACAA
CTCAGGTTCTGTTTGGGAACAACATCCGATTAACTGTCCCCTCATCTGTCCCCACTGTCAACTCTGTCAC
CCCAATAAACGCACGGCATATACCTAGGACTTTAAGTGCTGTTCCGCCATCTGCCTTAAAGCTGGCTGCT
GCAGCAAATTGTCAAGTTTCCAAGGTCCCCTCCTCATCCTCTGTAGATTCAGTTCCAAGGGAAAATCATG
AATCGGAAAAGCCAGCACTGAACAACATAGCAGACAACACAGTAGCAATGGAGGTGACG

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG215235 representing NM_027497
Red=Cloning site Green=Tags(s)

MSKLSFRARALDASKPLPVFRCEDLPDLHEYASINRAVPQMPTGMEKEEESEHHLQRAISAQQVYGEKRD
NMVIPVPEAESNIAYYESIYPGEFRMPKQLIHIQPFSLDAEQPDYDLDSEDEVFVNKLKKKMDICPLQFE
EMIDRLEKGSGQQPVSLQEAKLLLKEDDELIREVYEYWIKKRKTCRGSSLIPLVKQEKRDGSSTNDPYVA
FRRRTEKMQTRKNRKNDEASYEKMLKLRRDLSRAVTILEMIKRREKSKRELLHLTLEIMEKRYNLGDYSG
EIMSEVMAQRQPVKPTYAIPIIPITNSSQFKHQDATDSKEFKVNKQDKADLIRPKRKYEKKPKVLPPSAA
APQQQSPAALPGFSAKDLNQYDFPSSDEEPLSQVLSGSSEAEEENDPDGPFAFRRKAGCQYYAPHLDQTG
NWPWTSPKDGGLGDVRYRYCLTTLTVPQRCLGFARRRVGRGGRVVLDRAHSDYDSMFHHLDLDMLSSPQP
SPVNQFANTSEPNTSDRSSSKDLSQILVDIKSCRWRHFRPRTPSLPDSDSGELSSRKLHRSISRAGAAQP
GAHTCSTSTQNRSSSGSAHCAFTAEQYQQHQQQLALMQQQQLAQTQQQQQANSSSSAAAQQGFVSKTLDS
ASAQFAASALMTSEQLLGFKVKDDVVLGLGVNGVLPASGVYKGLHLSSTTPTALVHTSPSTAGSTLLQPS
NITQTSGSHSSLSHQVTAASSATTQVLFGNNIRLTVPSSVPTVNSVTPINARHIPRTLSAVPPSALKLAA
AANCQVSKVPSSSSVDSVPRENHESEKPALNNIADNTVAMEVT

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_027497
ORF Size 2439 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_027497.3, NP_081773.1
RefSeq Size 3983 bp
RefSeq ORF 2442 bp
Locus ID 13831
UniProt ID Q8C9X6
Cytogenetics 18 A1
Summary Component of the NuA4 histone acetyltransferase (HAT) complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histones H4 and H2A. This modification may both alter nucleosome - DNA interactions and promote interaction of the modified histones with other proteins which positively regulate transcription. This complex may be required for the activation of transcriptional programs associated with oncogene and proto-oncogene mediated growth induction, tumor suppressor mediated growth arrest and replicative senescence, apoptosis, and DNA repair. NuA4 may also play a direct role in DNA repair when directly recruited to sites of DNA damage (By similarity).UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_027497" in other vectors (4)
SKU Description Size Price
MC221798 Epc1 (untagged) - Mouse enhancer of polycomb homolog 1 (Drosophila) (Epc1), transcript variant 2, (10ug) 10 ug
$820.00
MR215235 Epc1 (Myc-DDK-tagged) - Mouse enhancer of polycomb homolog 1 (Drosophila) (Epc1), transcript variant 2 10 ug
$589.00 $745.00
MR215235L3 Lenti ORF clone of Epc1 (Myc-DDK-tagged) - Mouse enhancer of polycomb homolog 1 (Drosophila) (Epc1), transcript variant 2 10 ug
$1,045.00
MR215235L4 Lenti ORF clone of Epc1 (mGFP-tagged) - Mouse enhancer of polycomb homolog 1 (Drosophila) (Epc1), transcript variant 2 10 ug
$1,045.00

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.