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Ctsh (NM_007801) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG215191
Ctsh (tGFP-tagged) - Mouse cathepsin H (Ctsh), (10ug)
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ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $500.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Ctsh
Synonyms AL022844
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG215191 representing NM_007801
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTGGGCTGCGCTGCCGCTGCTGTGCGCTGGGGCCTGGCTGCTGAGTACTGGGGCCACCGCCGAGCTGA
CCGTGAACGCCATAGAAAAGTTTCACTTTAAGTCATGGATGAAACAGCATCAAAAGACGTACAGCTCGGT
GGAGTACAACCACAGACTGCAGATGTTTGCCAACAACTGGAGGAAGATTCAAGCCCACAACCAGAGGAAC
CACACATTTAAAATGGCATTGAACCAGTTTTCAGATATGAGCTTTGCTGAAATAAAACACAAATTCCTTT
GGTCAGAGCCTCAGAATTGCTCAGCCACCAAAAGTAACTACCTCCGAGGTACAGGCCCCTACCCTTCCTC
CATGGACTGGAGGAAGAAAGGAAATGTTGTTTCGCCAGTGATAAATCAGGGGGCCTGTGGCAGCTGCTGG
ACTTTCTCTACCACGGGGGCCCTAGAGTCAGCTGTGGCTATTGCCAGTGGGAAAATGCTGTCTTTGGCTG
AGCAGCAGCTGGTGGATTGTGCCCAAGCCTTCAACAATCATGGCTGCAAAGGAGGTCTCCCCAGCCAGGC
CTTCGAGTACATCCTATACAACAAGGGCATCATGGAAGAAGACAGCTACCCTTACATAGGCAAGGATAGT
TCATGCAGATTCAACCCCCAAAAAGCTGTTGCATTCGTCAAGAATGTTGTCAACATCACACTCAATGACG
AGGCTGCAATGGTTGAGGCTGTGGCTCTATACAACCCTGTGAGCTTCGCCTTTGAGGTGACTGAAGATTT
TTTGATGTATAAAAGTGGCGTCTACTCCAGTAAATCCTGTCATAAAACTCCAGATAAAGTAAACCATGCA
GTCCTGGCGGTTGGCTATGGAGAACAGAATGGATTACTCTACTGGATTGTGAAAAACTCTTGGGGCTCCC
AGTGGGGGGAGAATGGGTACTTCCTCATTGAACGTGGGAAGAACATGTGTGGCCTGGCTGCTTGTGCCTC
CTATCCCATTCCTCAGGTA

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG215191 representing NM_007801
Red=Cloning site Green=Tags(s)

MWAALPLLCAGAWLLSTGATAELTVNAIEKFHFKSWMKQHQKTYSSVEYNHRLQMFANNWRKIQAHNQRN
HTFKMALNQFSDMSFAEIKHKFLWSEPQNCSATKSNYLRGTGPYPSSMDWRKKGNVVSPVINQGACGSCW
TFSTTGALESAVAIASGKMLSLAEQQLVDCAQAFNNHGCKGGLPSQAFEYILYNKGIMEEDSYPYIGKDS
SCRFNPQKAVAFVKNVVNITLNDEAAMVEAVALYNPVSFAFEVTEDFLMYKSGVYSSKSCHKTPDKVNHA
VLAVGYGEQNGLLYWIVKNSWGSQWGENGYFLIERGKNMCGLAACASYPIPQV

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_007801
ORF Size 999 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_007801.2, NP_031827.2
RefSeq Size 1627 bp
RefSeq ORF 1002 bp
Locus ID 13036
UniProt ID P49935
Cytogenetics 9 47.4 cM
Summary This gene encodes a member of the peptidase C1 (papain) family of cysteine proteases. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate multiple protein products. These products include the cathepsin H mini, heavy, and light chains. In rat and human, these three chains can associate to form the mature enzyme, which has both aminopeptidase and endopeptidase activities. Homozygous knockout mice for this gene exhibit impaired lung surfactant processing and reduced tumorigenesis in a pancreatic cancer model. Multiple pseudogenes of this gene have been identified in the genome. provided by RefSeq, Aug 2015
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Documents
Resources
"NM_007801" in other vectors (3)
SKU Description Size Price
MR215191 Ctsh (Myc-DDK-tagged) - Mouse cathepsin H (Ctsh) 10 ug
$289.00 $300.00
MR215191L3 Lenti ORF clone of Ctsh (Myc-DDK-tagged) - Mouse cathepsin H (Ctsh) 10 ug
$600.00
MR215191L4 Lenti ORF clone of Ctsh (mGFP-tagged) - Mouse cathepsin H (Ctsh) 10 ug
$600.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.