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Mbd5 (NM_029924) Mouse Tagged ORF Clone

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SKU
MG215106
Mbd5 (tGFP-tagged) - Mouse methyl-CpG binding domain protein 5 (Mbd5), (10ug)
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ORF Plasmid:
tGFP DDK
  $1,652.00
3 Weeks*
Bulk Requests & Clone Modifications

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Other products for "Mbd5 or NM_029924"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Mbd5
Synonyms 9430004D19Rik; AA536666; AI426407; C030040A15Rik; Gm1630; OTTMUSG00000012483
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG215106 representing NM_029924
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAATGGAGGCAAAGAGTGTGACGGAGGAGACAAGGAAGGAGGTCTTGCAGCTATCCAGGTTCCTGTGG
GTTGGCAGCGGCGTGTGGACCATAATGGCGTGCTGTACATCAGTCCCAGTGGGTCTTTGTTATCTTGCTT
GGACCAGGTTAAGACATACCTGCTCACTGACGGAACATGCAAGTGTGGCTTGGAATGTCCTCTTATTCTT
CCCAAGGTGTTTAATTTTGATCCTGGAGCTGCTGTGAAGCAGAGAACTGCAGAAGATGTTAAGGCAGATG
ATGATGTCACAAAGCTATGCATACATAAAAGAAAGATCATTGCAGTGGCCACACTCCATCAAAGCATGGA
GGCCCCGCATCCTTCTCTGGTGCTCACCAGTCCTGGAGGAGGAACAAATGCAACTCCAGTAGTACCTTCA
CGGGCGGCAACTCCAAGATCAGTAAGAAACAAGTCTCATGAAGGAATTACAAATTCTGTAATGCCAGAAT
GTAAGAATCCTTTCAAGTTAATGACTGGATCATCAAATGCCATGGGAAGGCTATACATGCAAGACCTGCC
TGGAAGCCAGCAGCAGGAGCTCCACCCTGTCTACCCCCGCCAGAGATTGGGCAGTAGTGAGCATGGACAG
AAATCTCCATTTCGTGGCAGCCATGGAGGCCTGCCTAGCCCAGCATCATCAGGGTCTCAGATCTATGGAG
ATGGCTCAATCTCTCCAAGGACTGACCCGCTCGGAAGCCCTGATGTCTTCACAAGAAATAACCCTGGTTT
TCATGGAGCTCCCAATTCCAGCCCTATTCACCTAAATAGGACTCCTCTTTCTCCACCTTCAGTAATGCTA
CATGGTTCTCCCGTACAGTCATCCTGTGCAATGGCTGGAAGAACTAATATACCTCTTTCCCCAACCTTGA
CTACAAAGAGTCCAGTAATGAAAAAACCAATGTGTAATTTTTCAACTAATATGGAAATACCACGAGCAAT
GTTCCACCACAAACCACCCCAAGGCCCACCTCCCCCTCCTCCACCTTCTTGTGCTCTTCAGAAAAAGCCA
TTAACATCTGAGAAAGATCCACTTGGCATTCTTGACCCTATTCCTAGTAAACCAGTGAATCAGAACCCTA
TTATCATTAATCCAACCAGTTTCCATTCAAATGTCCACTCTCAGGTACCTGTGATGAATGTAAGCATGCC
TCCTGCTGTTGTTCCTTTGCCAAGTAATCTCCCTTTGCCAACTGTAAAACCTGGTCATATGAATCATGGG
AGTCATGTACAAAGAATTCAGCATTCAGCTTCAACCTCCCTGTCCCCTTCTCCAGTGACATCCCCAGTGC
ACATGATGGGGACTGGAATTGGAAGGATTGAGGCATCGCCCCAAAGATCACGCTCATCTTCCACATCATC
AGATCATGGAAATTTCATGATGCCACCTGTAGGACCCCAGGCCACTTGTAGTGGCATAAAGGTTCCACCT
AGGTCACCAAGGTCAACAATAGGTTCCCCAAGGCCATCAATGCCATCAAGCCCTTCTACCAAGTCTGATG
GACATCATCAGTACAAGGATATCCCTAACCCATTAATTGCTGGAATGAGTAATGTACTCAATACCCCAAG
CAGTGCAGCTTTTCCTACTGCACCTGCCGGAAATGGTTCTGTAAAGAGTCAGCCTGGTTTGCTGGGAATG
CCTTTAAATCAGATCTTGAACCAGCACAATGCTGCCTCCTTTCCAGCAAGTAGTTTACTCTCAGCAGCAG
CCAAAGCACAGCTAGCAAATCAAAACAAACTTGCTGGTAACAACAGTAGCAGCAGTAACAATTCTGGAGC
TGTTGCCAGCAGCGGCAACACTGAAGGACACAGCACTTTAAACACCATGTTCCCTCCAACTGCCAACATG
CTTCTCCCAACAGGTGAAGGTCAAAGTGGTCGAGCAGCACTAAGAGATAAGTTGATGTCTCAGCAAAAAG
ACTCATTACGGAAAAGAAAGCAGCCACCCACCACAGTGTTGAGTTTGCTCAGACAGTCCCAAATGGATAG
TTCTGCAGCTCCTAAACCTGGACCTGACTTGCTAAGGAAGCATGGGCAGGGGTCATTTCCCATCAGTTCA
ATGTCTCAGTTACTACAGTCTATGAGTTGTCAAAGCTCTCACTTGAGTAGCAATAGTACCCCGGGTTGTG
GGGGATCAAATACTGCTTTGCCTTGCTCTGCTAACCAGCTGCATTTTCCAGATCCTAATATGAATTCCAC
TGTTCTTCAGAATTCACTGACACAGAGCATACCTTTAAGAGGGGAAGCCGTGCACTGCCACAATGCAAAC
ACTAACTTTGTTCACAGTAACAGTCCAGTCCCCAACCACCATCTTGCAGGTTTAATAAATCAGATTCAGG
CTAGCGGGAACTGTGGGATGCTCAGTCAGTCGGGCATGGCTTTAGGAAACTCATTACATCCCAATCCACC
TCAGTCAAGAATTTCAACGTCCTCCACTCCAGTGATACCAAACAGCATTGTTAGCAGCTATAATCAAACA
AGTTCTGAAGCAGGTGGTTCAAGCCTGCCGTCCTCCATAGCTATAGCTGGCAGCAACCACCCTGCCATCA
CAAAGACAACATCTGTTCTCCAAGATGGTGTCATAGTCACCACCGCAGCTGGAAACCCACTGCAGAGTCA
GCTGCCCATTGGGAGTGACTTTCCTTTTGTTGGCCAAGAGCATGCCCTTCATTTTCCATCCAACAGCACC
GCAAACAACCATCTTCCACACCCCTTGAACCCCAGCCTCCTCAGCTCTCTACCTATCTCTTTGCCAGTGA
ATCAACAGCATCTCCTAAACCAGAATCTATTAAATATCCTCCAGCCTTCAGCAGGAGAAGGCAAGTCTGA
GATCAACCTCCACCCTTTAGGTTTTCTCAACCCGAATGTAAACGCTGCTTTAGCTTTCCTCTCCGGTGAC
GTGGATGGGCAGGTATTACAGCCTGTCCACTTTCAGCTCTTAGCAGCCCTGCTTCAGAACCAAGCCCAAG
CAGCTGCCATGCTTCCCCTGCCGTCTTTCAATCTGACCATCTCAGATCTTTTGCAACCGCAAAATACCCC
TTTACCCTTATTAACACAGATGACAGCCCCACCAGACCGTTTGCCAAGCAATCAGTCAGACAACAGCCGA
GCTGATACCCTTTTAGGCAGCCCCCTGGGAAACCCTTTAGCAGGCAGTGACACTACTTTTAACCCCCTGC
TCCTCCCAGCTGTCACTGGGGCCTCAGGATTAATGGCCTTGAATCCCCAGCTGTTGGGGGGTGTCCTGAA
CTCGGCATCGGCCAACACCGCTATTCATCCAGAGGTTTCCATAGCAACCTCCTCCCAGGCAACCACTACC
ACAACCACTACATCATCAGCAGTGGCAGCACTGACTGTCTCAACACTTGGTGGGACAGCAGTGGTGTCAA
TGGCAGAAACATTGCTGAATATATCTAATAATGCTGGGAATCCACCTGGTCCAGCTAAACTCAACAGTAA
CTCTATGGTGCCACAGCTACTTAACCCTCTACTGGGGACAGGTCTGCTTGGTGATATATCATCCATAAAC
AATTCATTGAATAACCATCAACTGACTCATCTACAGTCGCTACTAAACAGCAATCAGATGTTTCCTCCAA
ATCAGCAGCCGCAGCAACACCTTCTCCAGGGACACCAGAATCTCCAGGCCTTCCAAGGACAACCCACAGT
CCCTTGCCCAGCTAACAATAACCCCATGGCTTGTCTGTTTCAGAACTTCCAGGTGAGGATGCAAGGAGAT
GCAGCTCTTCTAAATAAAAGAATAAGCACTCAGCCTGGGCTCACAACACTTCCTGAAAACCCAAACCTTG
CACTTCCACATTTCCAAGATACACCCTGTGAGCTGCAGCCAAGGATTGACCTTGGTCAGCCTATGAAGGA
CGGCCTCGTTATGGGTGGCCAAGGAGATGCCTCCGTAGACGCCATTTACAAAGCAGTTGTGGATGCAGCC
AGCAAAGGAATGCAGGTTGTCATCACCACTGCTGTCAACAGCACGACTCAAATCAGCCCTATCCCAGCCC
TGAGCGCCATGAGTGCCTTCACTGCCTCCATTGGTGACCCATTAAATCTGTCCAGTGCTGTGAGCGCTGT
CATCCACGGGCGGAACATGGGAGGAGTGGATCATGATGGCAGGCTGAGGAATGCGAGAGGGGCACGGCTA
CCCAAGAACATAGACCATGGGAAGAACTCCAGCGAAGGAGACGGGTTTGAGTGTTTCAAGTCAGCGAGCT
GCCACACATCCAGAAAACAGTGGGATGGGGAGCAAAGTCCCAGAGGGGAGCGCAACAGGTGGAAGTATGA
GGAGTTTTTAGATCATCCAGGCCATATCCACAGTAGTCCTTGCCACGAAAGGCCCAACAATGTCTCTACA
CTGCCATTTCTAGCTGGGGAACAGCACCCGATACTGTTACCACCAAGAAACTGTCAAGGTGATAAGATTC
TGGAGGAAAATTTCAGGTATAATAATTACAAAAGAACTATGATGAGTTTTAAAGAGAGACTAGAGAGTAC
TGTGGAGAGATGTACACACATTAACGGAAACAGACCTCGTCAGAGTCGGGGCTTTGGAGAGCTGCTAGGC
ACTGCAAAACAAGACCTAGTTCTAGAGGGGCAGTCTCCGGGTTCTTCAAATAGTTTGGAAAGTTCTCTGG
TCAAAGACTACATCCATTACAATGGAGACTTTAATGCCAAAACCATTAATGGGTGTGTGCCTAGTCCCTC
AGATGCTAAAAGCATTAGTAGTGAAGACGACCTACGGAATCCAGACTCCCCCTCCTCACATGAGTTGATA
CACTATAGACCAAGGACGTTCAACGTTGGCGACTTGGTCTGGGGCCAAATCAAAGGACTGACTTCCTGGC
CTGGGAAATTCATAAGAGAAGACGACGTTCACAATTCATGCCAACAAAGCCCCGAGGAAGGGAAGGTGGA
GCCCGAGAAGTTGAAGACTCTCACAGAAGGTTTAGAAGCCTACAGCCGCGTCCGGAAAAGGAGCAGAAAA
AGTGGGAAACTAAATAACCATTTAGAAGCTGCTATTCACGAGGCCATGAGCGAACTGGACAAAATGTCTG
GGACTGTTCACCAAATCCCACAGGGTGACAGACAAATGAGGCCCCCCAAACCCAAGAGGAGGAAGATCTC
CAGA

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG215106 representing NM_029924
Red=Cloning site Green=Tags(s)

MNGGKECDGGDKEGGLAAIQVPVGWQRRVDHNGVLYISPSGSLLSCLDQVKTYLLTDGTCKCGLECPLIL
PKVFNFDPGAAVKQRTAEDVKADDDVTKLCIHKRKIIAVATLHQSMEAPHPSLVLTSPGGGTNATPVVPS
RAATPRSVRNKSHEGITNSVMPECKNPFKLMTGSSNAMGRLYMQDLPGSQQQELHPVYPRQRLGSSEHGQ
KSPFRGSHGGLPSPASSGSQIYGDGSISPRTDPLGSPDVFTRNNPGFHGAPNSSPIHLNRTPLSPPSVML
HGSPVQSSCAMAGRTNIPLSPTLTTKSPVMKKPMCNFSTNMEIPRAMFHHKPPQGPPPPPPPSCALQKKP
LTSEKDPLGILDPIPSKPVNQNPIIINPTSFHSNVHSQVPVMNVSMPPAVVPLPSNLPLPTVKPGHMNHG
SHVQRIQHSASTSLSPSPVTSPVHMMGTGIGRIEASPQRSRSSSTSSDHGNFMMPPVGPQATCSGIKVPP
RSPRSTIGSPRPSMPSSPSTKSDGHHQYKDIPNPLIAGMSNVLNTPSSAAFPTAPAGNGSVKSQPGLLGM
PLNQILNQHNAASFPASSLLSAAAKAQLANQNKLAGNNSSSSNNSGAVASSGNTEGHSTLNTMFPPTANM
LLPTGEGQSGRAALRDKLMSQQKDSLRKRKQPPTTVLSLLRQSQMDSSAAPKPGPDLLRKHGQGSFPISS
MSQLLQSMSCQSSHLSSNSTPGCGGSNTALPCSANQLHFPDPNMNSTVLQNSLTQSIPLRGEAVHCHNAN
TNFVHSNSPVPNHHLAGLINQIQASGNCGMLSQSGMALGNSLHPNPPQSRISTSSTPVIPNSIVSSYNQT
SSEAGGSSLPSSIAIAGSNHPAITKTTSVLQDGVIVTTAAGNPLQSQLPIGSDFPFVGQEHALHFPSNST
ANNHLPHPLNPSLLSSLPISLPVNQQHLLNQNLLNILQPSAGEGKSEINLHPLGFLNPNVNAALAFLSGD
VDGQVLQPVHFQLLAALLQNQAQAAAMLPLPSFNLTISDLLQPQNTPLPLLTQMTAPPDRLPSNQSDNSR
ADTLLGSPLGNPLAGSDTTFNPLLLPAVTGASGLMALNPQLLGGVLNSASANTAIHPEVSIATSSQATTT
TTTTSSAVAALTVSTLGGTAVVSMAETLLNISNNAGNPPGPAKLNSNSMVPQLLNPLLGTGLLGDISSIN
NSLNNHQLTHLQSLLNSNQMFPPNQQPQQHLLQGHQNLQAFQGQPTVPCPANNNPMACLFQNFQVRMQGD
AALLNKRISTQPGLTTLPENPNLALPHFQDTPCELQPRIDLGQPMKDGLVMGGQGDASVDAIYKAVVDAA
SKGMQVVITTAVNSTTQISPIPALSAMSAFTASIGDPLNLSSAVSAVIHGRNMGGVDHDGRLRNARGARL
PKNIDHGKNSSEGDGFECFKSASCHTSRKQWDGEQSPRGERNRWKYEEFLDHPGHIHSSPCHERPNNVST
LPFLAGEQHPILLPPRNCQGDKILEENFRYNNYKRTMMSFKERLESTVERCTHINGNRPRQSRGFGELLG
TAKQDLVLEGQSPGSSNSLESSLVKDYIHYNGDFNAKTINGCVPSPSDAKSISSEDDLRNPDSPSSHELI
HYRPRTFNVGDLVWGQIKGLTSWPGKFIREDDVHNSCQQSPEEGKVEPEKLKTLTEGLEAYSRVRKRSRK
SGKLNNHLEAAIHEAMSELDKMSGTVHQIPQGDRQMRPPKPKRRKISR

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_029924
ORF Size 5184 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_029924.2, NP_084200.1
RefSeq Size 7055 bp
RefSeq ORF 5187 bp
Locus ID 109241
Cytogenetics 2 C1.1
Summary Binds to heterochromatin. Does not interact with either methylated or unmethylated DNA (in vitro) (By similarity).UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_029924" in other vectors (2)
SKU Description Size Price
MC224878 Mbd5 (untagged) - Mouse methyl-CpG binding domain protein 5 (Mbd5), (10ug) 10 ug
$1,598.00
MR215106 Mbd5 (Myc-DDK-tagged) - Mouse methyl-CpG binding domain protein 5 (Mbd5) 10 ug
$1,452.00

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