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Svs2 (NM_017390) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG215004
Svs2 (tGFP-tagged) - Mouse semenogelin I (Semg1), (10ug)
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ORF Plasmid:
tGFP DDK
  $703.00
3 Weeks*
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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Svs2
Synonyms BB115391; Semg1; SvsII
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG215004 representing NM_017390
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAAGTCCTCTGTCTTCGTCCTTTCTCTGCTCCTCATTCTGGAAAGACAGTCAGCTGTGGTTGGACAAT
ATGGGGCTACAAAAGGCCACTTCCAGAGCAGCTCCTCAGAGGGATTTATGCTTGGTCAGAAAGGCCGTCT
CAGTTTCGGGATCAAAGGAGGAAGTGACGAAGCAGCTGAAGAAAGCCTTTTCATGCAATCACAACGCCGG
GTGTATGGCCAGGGTGGTGGTGACATGACCCAGACACGTGTTTCACAAGAGCATACAAGTGTCAAAGGGG
CCGCACTTTGTCGTAATGGACAAGTATCCCAACTGAAATCCCAAGAATCCCAAATAAAATCCTATGGCCA
AGTGAAATCCAGTGGACAGCTGAAATCTGGAGGATCAGCCTTTGGACAAGTGAAGTCCAGTGTGTCCCAA
ATAAAATCCTATGGACAGCTGAAATCCGGTGGTCAGTTGAAATCTGGAGGACCAGCCTTTGGACAAGTGA
AATCCCAAGAATCCCAAATAAAATCTTATGGACAATTGAAATCCAGTGGACAACTGAAATCTGGAGGATC
GGCCTTTGGACAAGTGAAGTCCAGTGTGTCCCAAATAAAATCCTATGGACAGCTGAAATCCGGTGGATCC
CAAGTAAAATCCTATGGTCAAACAAAATCCTATGGTGAAGAAGGTCAACTGAACTCTTTCAGCCAACTAA
AATCCCAAGGTGCCCAACTAAAATCCTATGGTCAACAAAAATCTCAACAACAGTCCTCCTTTAGCCAAGT
AAAATCTCAAAGTTCCCAACTAAAGTCCTATGGCCAACAAAAATCCCTCAAAGGTTTTTCTCAACAAACT
CAGCATAAAGGATTTGCCATGGATGAAGGTATGTCACAAGTGCGGAAACAATTTAGTGACGATGACCTCT
CAGTACAACAGAAGTCTACCCAACAGATGAAAACAGAGGAAGACTTATCCCAATTCGGACAACAGCGGCA
ATATGGACAAGAGCGCTCACAATCCTATAAAGGATATCTTGAGCAATACAGAAAGAAAGTACAAGAACAA
CAACGAAAAAATTTTAATCCCGGTAACTATTTTACAAAGGGAGGAGCAGACCTCTATCAAGCTCAGCTTA
AGGGA

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG215004 representing NM_017390
Red=Cloning site Green=Tags(s)

MKSSVFVLSLLLILERQSAVVGQYGATKGHFQSSSSEGFMLGQKGRLSFGIKGGSDEAAEESLFMQSQRR
VYGQGGGDMTQTRVSQEHTSVKGAALCRNGQVSQLKSQESQIKSYGQVKSSGQLKSGGSAFGQVKSSVSQ
IKSYGQLKSGGQLKSGGPAFGQVKSQESQIKSYGQLKSSGQLKSGGSAFGQVKSSVSQIKSYGQLKSGGS
QVKSYGQTKSYGEEGQLNSFSQLKSQGAQLKSYGQQKSQQQSSFSQVKSQSSQLKSYGQQKSLKGFSQQT
QHKGFAMDEGMSQVRKQFSDDDLSVQQKSTQQMKTEEDLSQFGQQRQYGQERSQSYKGYLEQYRKKVQEQ
QRKNFNPGNYFTKGGADLYQAQLKG

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_017390
ORF Size 1125 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_017390.4, NP_059086.3
RefSeq Size 1570 bp
RefSeq ORF 1128 bp
Locus ID 53878
Cytogenetics 2 84.82 cM
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Documents
Resources
"NM_017390" in other vectors (2)
SKU Description Size Price
MC209952 Svs2 (untagged) - Mouse seminal vesicle secretory protein 2 (Svs2), (10ug) 10 ug
$503.00
MR215004 Svs2 (Myc-DDK-tagged) - Mouse seminal vesicle secretory protein 2 (Svs2) 10 ug
$503.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.