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Krt90 (NM_177717) Mouse Tagged ORF Clone

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SKU
MG214959
4732456N10Rik (tGFP-tagged) - Mouse RIKEN cDNA 4732456N10 gene (4732456N10Rik), (10ug)
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ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $702.00
3 Weeks*
Bulk Requests & Clone Modifications

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Other products for "Krt90 or NM_177717"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Krt90
Synonyms 4732456N10Rik; Ket90
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG214959 representing NM_177717
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGACCCAGCGATCCTCGGTGACCATCAAGTCTGGGGGCACGCGGAACTTCAGTGCCTCCTCGGCTAGCC
TCCTTCCTGGATGTCGGCCGGGTTTCAGCTCTGTCTCTGTGTCCCAGAGCGGGAAGAGTTTCGGAGGTGG
CATAGGGGGTGGTTTTGGGACAAGAAGCCTACACAGCTTTGGGGGTAACAAGAGGATCTCCATCGGTGGG
GGCTACCGCTCCACCCGAGCCAGCTTTGGGGGCTCTGCCTGTGGTCTGGGTGTCAGCGGCATAGGATACA
GAGTTGGGGGAGCCTATGGAGGGTATGGATATGGAGGTGGGATGGCCCCTGGAGCTGGAGGCATCCATGA
AGTCACTGTCAACCAGAGTCTCCTGACACCCCTCCACCTGGAGATTGATCCATCTCTCCAGCGGGTTCGA
AAGGAGGAGAAGGAGCAGATCAAGACCCTCAACAACAAGTTCGCCTCCTTCATAGATAAGGTGCGGTTCT
TGGAGCAGCAGAACAAAGTCTTAGAGACCAAATGGAGCCTCCTCCAAGAGCACAAAACAACCAGGACCAA
TCTAGAGCCCATGTTCGAAGCCTACATCACCAACCTGAGGCGCCAGCTGGAGTGCCTGGGAGGGGAGCGG
AGCAGGCTGGAGACGGAGCTGAAGAGCATGCAGGACGTGGTGGAGGACTTCAAGAACAAGTACGAAGAGG
AAATCCACAGACGTACAGCGGCAGAGAATGAGTTTGTGGTACTCAAAAAAGATGTGGACGCTGCCTACAT
GAACAAGGTGGAGCTAGAGGCCAAGGTGGAAGCTCTGATGGATGAGATCAACTTCCTGAGAGCTTTCTTT
GAAGCGGAGCTGGCTCAACTTCAGGCTCAAATCTCTGAGACCTCTGTGGTCCTGTCCATGGACAACAACC
GCAGCCTGAACCTGGACAGCATCATTGCTGAGGTCAAGGCCCAATACGAGGACATTGCCAATCGCAGCCG
GGCAGAGGCTGAATCCTGGTACCAGACCAAGTATGAGGAGCTGCAGAGGTCTGCTGGCCAACGCGGGGAT
GACCTCCGTACTACTAAGATGGAGATCTCTGAGCTGAACCGGGCCATGCAGAGGCTGCGTTCTGAGATTG
ACAATCTGAAGAAGCAGTGTGCCACACTCCAGGCTTCCATTGCCGATGCCGAGCAACGCGGGGAGCTGGC
TCTCAAAGATGCCAAGAACAAGCTGGCCGAGCTGGAGGACGCCCTGCAGAAGGCCAAGCAGGACATGGCC
CGTCAACTGCGAGAGTACCAGGAGCTCATGAACGTCAAGCTGGCCCTGGACATAGAGATCGCCACCTACC
GAAAGCTGCTGGAGGGCGAGGAGTGCAGGCTCACTGGAGAAGGTGTTGGTGCCGTGAACATCTCTGTGGT
CTCTTCCTCCGGGGGCACAGGCTACAGTGGAGGTGGTGGCCTGTGTGTGAGTGGCGGAGGCTACAGCGGA
GGCGGCTACAGTGGGAGTGGCCTCTGCTACGGGGGCGGAGGCAGCGGAGGTTTTAGCTCCACCAGCGGTC
GCAGTGTGAGCGGCAGCAGTTCAAGCATGAGAATTGTCTCCAAGACATCATCCACCAAGAAGAGTTACCG
GAGC

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG214959 representing NM_177717
Red=Cloning site Green=Tags(s)

MTQRSSVTIKSGGTRNFSASSASLLPGCRPGFSSVSVSQSGKSFGGGIGGGFGTRSLHSFGGNKRISIGG
GYRSTRASFGGSACGLGVSGIGYRVGGAYGGYGYGGGMAPGAGGIHEVTVNQSLLTPLHLEIDPSLQRVR
KEEKEQIKTLNNKFASFIDKVRFLEQQNKVLETKWSLLQEHKTTRTNLEPMFEAYITNLRRQLECLGGER
SRLETELKSMQDVVEDFKNKYEEEIHRRTAAENEFVVLKKDVDAAYMNKVELEAKVEALMDEINFLRAFF
EAELAQLQAQISETSVVLSMDNNRSLNLDSIIAEVKAQYEDIANRSRAEAESWYQTKYEELQRSAGQRGD
DLRTTKMEISELNRAMQRLRSEIDNLKKQCATLQASIADAEQRGELALKDAKNKLAELEDALQKAKQDMA
RQLREYQELMNVKLALDIEIATYRKLLEGEECRLTGEGVGAVNISVVSSSGGTGYSGGGGLCVSGGGYSG
GGYSGSGLCYGGGGSGGFSSTSGRSVSGSSSSMRIVSKTSSTKKSYRS

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_177717
ORF Size 1614 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_177717.4, NP_808385.2
RefSeq Size 2534 bp
RefSeq ORF 1617 bp
Locus ID 239673
Cytogenetics 15 F2
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Documents
Resources
"NM_177717" in other vectors (4)
SKU Description Size Price
MC218635 4732456N10Rik (untagged) - Mouse RIKEN cDNA 4732456N10 gene (4732456N10Rik), (10ug) 10 ug
$552.00
MR214959 4732456N10Rik (Myc-DDK-tagged) - Mouse RIKEN cDNA 4732456N10 gene (4732456N10Rik) 10 ug
$289.00 $502.00
MR214959L3 Lenti ORF clone of 4732456N10Rik (Myc-DDK-tagged) - Mouse RIKEN cDNA 4732456N10 gene (4732456N10Rik) 10 ug
$802.00
MR214959L4 Lenti ORF clone of 4732456N10Rik (mGFP-tagged) - Mouse RIKEN cDNA 4732456N10 gene (4732456N10Rik) 10 ug
$802.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.