OriGene Logo

Bbof1 (NM_028377) Mouse Tagged ORF Clone

  • TrueORF®
Be the first to review this product
SKU
MG214879
Ccdc176 (tGFP-tagged) - Mouse RIKEN cDNA 2900006K08 gene (2900006K08Rik), (10ug)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $696.00
3 Weeks*
Bulk Requests & Clone Modifications

Frequently Bought Together (4)

TA150041 Mouse monoclonal turboGFP antibody, clone OTI2H8
100 ul
$447.00
CC100003 DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
5 x 200 ul
$170.00
PRIMER Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
100 pmol
$59.00
PS100010 pCMV6-AC-GFP, mammalian vector with C-terminal tGFP tag, 10ug
10 ug
$780.00

Other products for "Bbof1 or NM_028377"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Bbof1
Synonyms 2900006K08Rik; AI427784; Ccdc176
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG214879 representing NM_028377
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCCGGCGAAGGACAAGAGGAAAGACAAGAGGAAAGACAAGAGGAAAGGCAAAAACAAAGGCAAAGAAC
CAAAGAAAATAATCAAATCAGATGAACCTGCAATTGAAAGAGCCAAAGCCAATGCCTCTCTTTGGGAGGC
CAGATTGGAAGTCACAGAACTCTCTAGGATTGAATATCGTGATACTTCCCGAAGACTGGCAAAAAATAAT
GAAGACTTAAAGAAACAGCAATATAACATGGAAAAAGACATTATGTCTGTATTAAGCTACTTGAAGAAGC
AGGATCAAGAGAAAGATAATATGATAGAAAAACTGAAGCAGCAGTTGGCTAAAACAAAGGAAAAAGCCCA
GGAAGAGAAGGAAAAATTGGAACAAAAGTATGCTCTGCAAGTGAGTGAGTTGGAGGGACAGTTTCATCAA
AAAGCCAAGGAAATCGGCATGATTCAGACAGAGCTAAAGACAATAAAACAATTCCAGAAGAGAAAAATAC
AAGTGGAGAAAGAATTAGATGATCTGAAAGAAAATTTAAGGAACTCAGAGAAGAACTATCAGGAGACTCT
TAGAAGACTAGAAAGCAGATTTTTTGAAGAAAAGCACCGTTTGGAGCAAGAGGCTGAGAAGAGGATCATA
ATGCTGGCAGAGAGAGCTCACCATGAAGCTGTAGTGCAGTTGAACACTGCTGGAAGAAATGTTTTCAAAG
AGAATGTTTATCTTCACAAGGCGCTTGCATACCACTTGAAGGAAGCTGAAATTCTCCAGCAAAACTCCAA
GAAGATTGAAGAAAACCATTCCTGCCTTTTACAACAAAAGGAAATCAATGATCTGTTGGTTAAGGAAAAG
ATTATGCAACTCACCCAGCAGAAATCACAAATCCAAACTCTCCAGAAGAAGGTGGTAAGCTTGGAGAACG
CTCTGACCTACATGACCACAGAGTTTGAGGCTGAAGTGTTGAAACTGCAGCAAAAGGCAATCATAGAGAA
CCAAGCAGGCTTGGTAGAGATTGACAAGCTGCAGCAACTTCTTCAGATGAAGGACAGGGAGATGAACCGA
GTGAAGAGGCTGGCTAAGAACATACTGGATGAGAGGACAGAAGTGGAACAGTTCTTTCTAGACGCTCTCT
ACGAAGTGAAGCAACAGATTCTGGCCAGCAGGAAGCATTATAAGCAGATAGCACAAGCTGCTTTCAATTT
AAAAATGAGAGCAGCATGTGCGGGAAAAACAGAATATCCCAGAATCAGGACCTTTGATGGCAAAGAACAA
AGTACCAATAGTGTGAACCAGGATCTCTTGGAGGCTGAAAAATGGCCAACTACTCAAAAAAATGTGGATA
TTAGAGATTTGACCTGGGAGCAGAAGGAAAAGGTCTTAAGATTGCTCTTTGCGAAAATGAATGGTTTTGC
TGCTAGGAAATACAGTCAGAGTTCTAAGCCTCCTGTTCCAGACCATATTATTTATGGCAGTGGAGAAATG
AAAGAAACTGGGGATGAAAATAACCTTCTAGATCAAACCTTCATCACCCAGCAAGCGCCAGTATCAGACT
CCAATAGAATGGTGTCACCCGATGTTATTCCTCAAGGATTACAGGACTCTGACATAGCC

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG214879 representing NM_028377
Red=Cloning site Green=Tags(s)

MPAKDKRKDKRKDKRKGKNKGKEPKKIIKSDEPAIERAKANASLWEARLEVTELSRIEYRDTSRRLAKNN
EDLKKQQYNMEKDIMSVLSYLKKQDQEKDNMIEKLKQQLAKTKEKAQEEKEKLEQKYALQVSELEGQFHQ
KAKEIGMIQTELKTIKQFQKRKIQVEKELDDLKENLRNSEKNYQETLRRLESRFFEEKHRLEQEAEKRII
MLAERAHHEAVVQLNTAGRNVFKENVYLHKALAYHLKEAEILQQNSKKIEENHSCLLQQKEINDLLVKEK
IMQLTQQKSQIQTLQKKVVSLENALTYMTTEFEAEVLKLQQKAIIENQAGLVEIDKLQQLLQMKDREMNR
VKRLAKNILDERTEVEQFFLDALYEVKQQILASRKHYKQIAQAAFNLKMRAACAGKTEYPRIRTFDGKEQ
STNSVNQDLLEAEKWPTTQKNVDIRDLTWEQKEKVLRLLFAKMNGFAARKYSQSSKPPVPDHIIYGSGEM
KETGDENNLLDQTFITQQAPVSDSNRMVSPDVIPQGLQDSDIA

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_028377
ORF Size 1599 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_028377.3, NP_082653.2
RefSeq Size 2190 bp
RefSeq ORF 1602 bp
Locus ID 72873
UniProt ID Q3V079
Cytogenetics 12 D1
Summary Basal body protein required in multiciliate cells to align and maintain cilia orientation in response to flow. May act by mediating a maturation step that stabilizes and aligns cilia orientation. Not required to respond to planar cell polarity (PCP) or flow-based orientation cues (By similarity).UniProtKB/Swiss-Prot Function
Reviews
Write Review
 
 
 
 
 

Be the first to review this product

Please note:
Only reviews with images are eligible for a $20/20€ Amazon gift card.
Reviews without images are eligible for a $10/10€ Amazon gift card.
For more details about the terms and conditions, please visit the promotion page.

Documents
Resources
"NM_028377" in other vectors (4)
SKU Description Size Price
MC217615 Ccdc176 (untagged) - Mouse RIKEN cDNA 2900006K08 gene (2900006K08Rik), (10ug) 10 ug
$547.00
MR214879 Ccdc176 (Myc-DDK-tagged) - Mouse RIKEN cDNA 2900006K08 gene (2900006K08Rik) 10 ug
$289.00 $496.00
MR214879L3 Lenti ORF clone of Ccdc176 (Myc-DDK-tagged) - Mouse RIKEN cDNA 2900006K08 gene (2900006K08Rik) 10 ug
$796.00
MR214879L4 Lenti ORF clone of Ccdc176 (mGFP-tagged) - Mouse RIKEN cDNA 2900006K08 gene (2900006K08Rik) 10 ug
$796.00

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.