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Lipi (NM_177142) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG214834
Lipi (tGFP-tagged) - Mouse lipase member I (Lipi), (10ug)
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ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $350.00
3 Weeks*
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Other products for "Lipi or NM_177142"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Lipi
Synonyms D930038D03Rik; lpd1
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG214834 representing NM_177142
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAGAAATGGCTCAATTTCATTTGGTTTATTAAATGACCTTGGAGATTTGGAATACTCAACACTCTATG
AGAAGAGCAAGCCATTTGATAATCTTCAAGAAGTCAAGATTCTTGTTCAGTTCGTAAATGACATTGTGAG
CATTTCGCGCATTTGCTTGACATACTTTCAGTCCACAAATCCTTACTGTGCCGCGTGCCAGTACAAAATA
CAGAGCCTTGTGTTAAAATCACTCACATATCCAGAAAGACCACCAATTTGTAAGTATAATTTTGTATTGA
AAGAAGGAACAAGGGTAGATCTTCAACCAGATGAATGTAACACGCAGATGGAG

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG214834 representing NM_177142
Red=Cloning site Green=Tags(s)

MRNGSISFGLLNDLGDLEYSTLYEKSKPFDNLQEVKILVQFVNDIVSISRICLTYFQSTNPYCAACQYKI
QSLVLKSLTYPERPPICKYNFVLKEGTRVDLQPDECNTQME

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_177142
ORF Size 333 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_177142.4, NP_796116.1
RefSeq Size 1492 bp
RefSeq ORF 336 bp
Locus ID 320355
Cytogenetics 16 C3.1
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Documents
Resources
"NM_177142" in other vectors (4)
SKU Description Size Price
MC214457 Lipi (untagged) - Mouse lipase, member I (Lipi), (10ug) 10 ug
$165.00
MR214834 Lipi (Myc-DDK-tagged) - Mouse lipase, member I (Lipi) 10 ug
$289.00
MR214834L3 Lenti ORF clone of Lipi (Myc-DDK-tagged) - Mouse lipase, member I (Lipi) 10 ug
$450.00
MR214834L4 Lenti ORF clone of Lipi (mGFP-tagged) - Mouse lipase, member I (Lipi) 10 ug
$450.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.