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Osbpl8 (NM_001003717) Mouse Tagged ORF Clone

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SKU
MG214742
Osbpl8 (tGFP-tagged) - Mouse oxysterol binding protein-like 8 (Osbpl8) transcript variant 2, (10ug)
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ORF Plasmid:
tGFP DDK
  $976.00
3 Weeks*
Bulk Requests & Clone Modifications

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Other products for "Osbpl8 or NM_001003717"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Osbpl8
Synonyms AA536976; AA536995; C730029P18Rik; D330025H14Rik; OR; ORP-8
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG214742 representing NM_001003717
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGGCAGCCTTAGCAGACGGAGAACCTGACAGATCTTCGCTTCTTGGTGATAGCAAAGATGTCCTTG
GGCCATCAACTGTTGTAGCGAACAGTGACGAACCTCAGCATTTGACCCCAGGAAAGATGAGTCAGCGCCA
AGGCAGAGACGCTAACCCAACCCCAACTAGAGATCTGCCTCAGCCTTCTCTCAGCCCCGCCAGCCTTCAC
AGCCAGGGTTTTGAAAGAGGAAAAGAAGACATTTCTCAAAATAAAGATGACTCTTCACTTTCAATGTCAA
AGAGCAAGTCTGAATCTAAACTATATAATGGCTCAGAAAAGGACAGTTCAACTTCAAGTAAACTCACGAA
GAAAGAGTCTCTTAAGGTACAAAAGAAAAACTACCGTGAAGAGAAGAAGAGAGCCACGAAGGAGTTACTC
AGCACCATCACAGACCCCTCTGTCATTGTCATGGCCGACTGGCTGAAGATTCGTGGTACCCTGAAGAGCT
GGACCAAGCTGTGGTGTGTGTTGAAACCTGGCGTGCTTCTGATCTATAAAACACAAAAAAATGGTCAGTG
GGTCGGAACAGTCCTTCTGAATGCTTGTGAGATCATTGAACGTCCATCAAAAAAGGATGGCTTTTGTTTC
AAGCTTTTCCATCCTTTGGAGCAGTCTATTTGGGCAGTGAAGGGTCCTAAAGGTGAAGCGGTTGGCTCCA
TAACCCAGCCTCTACCTAGCAGCTACTTGATTATCCGAGCTACTTCAGAGTCAGATGGAAGGTGCTGGAT
GGATGCTTTGGAGTTGGCTTTGAAGTGTTCCAGTCTCCTTAAGCGGACCATGGTGAGGGAGGGGAAGGAG
CACGACCTGAGCATCTCCTCAGACAGCACACACGTGACGCTGTATGGCTTACTGCGCGCTAACAATCTCC
ACAGTGGCGACAACTTCCAGTTGAATGACAGTGAGATCGAAAGACAGCACTTTAAGGACCAAGATTTGTA
CTCTGATAAATCTGACAAGGAGAACGACCCGGAGCACGATGAGTCTGACAACGAGGTGCTGGGGAAGAGC
GAGGAGAGCGACACAGACACGTCCGAAAGGCAAGACGACTCGTACATCGACCCTGAGCCTGTGGAGCCCT
TAAAGGAGACCACCTACATGGAGCAGAGCCACGAAGAACTGGGAGAGGCAGGTGAGGCCTCTCAAACGGA
AACTGTGTCTGAAGAAAACAAAAGCCTTATCTGGACGCTGCTCAAGCAAGTCCGTCCCGGCATGGACCTA
TCCAGGGTGGTTCTTCCTACGTTTATTTTGGAGCCCCGCTCTTTCCTGGATAAGCTTTCAGATTACTACT
ACCACGCCGATTTCCTGTCTGAAGCTGCTCTAGAAGAAAATCCGTATTTCCGTTTGAAGAAAGTCGTGAA
ATGGTATTTGTCAGGATTTTATAAGAAGCCAAAGGGACTAAAGAAACCATATAATCCTATACTTGGTGAG
ACTTTCCGATGTTTATGGATTCATCCCAGAACAAACAGCAAAACTTTCTATATTGCTGAACAGGTGTCCC
ATCACCCACCAATATCTGCCTTTTATGTCAGTAACCGGAAAGATGGGTTTTGCCTTAGTGGTAGCATCCT
GGCTAAGTCCAAGTTTTATGGAAACTCGTTATCTGCCATATTGGAGGGAGAAGCCCGGTTAACTTTCTTG
AACAGAGGTGAAGATTACGTCATGACAATGCCATATGCTCACTGTAAAGGAATTCTTTATGGCACAATGA
CCCTGGAGCTTGGTGGAACTGTCAATATTACCTGTCAGAAGACTGGATACAGTGCGATACTTGAATTTAA
ACTAAAGCCATTTCTAGGGAGTAGTGATTATGTTAACCAAATATCAGGGAAACTTAAATTGGGAAAAGAA
GTCCTAGCTACTTTGGAAGGCCATTGGGATAGTGAAGTTTTCATTAATGACAAAAAGACTGATAACTCAG
AGATTTTCTGGAATCCAACTCCTGACATTAAACAGTGGAGATTAATAAGGCACACTGTAAAGTTTGAAGA
ACAGGATGATTTCGAATCAGAGAAACTCTGGCAACGAGTGACAAAAGCTATAAATGCCAAAGACCAAACG
GAAGCTACTCAAGAAAAGTATGTTCTGGAAGAAGCTCAAAGACAAGCTGCCAGAGATCGAAAAACAAAAA
CTCAAGAGTGGGTTTGTAAGTTATTTGAACTTGACCCACTCACTGGAGAATGGCACTATAAGTTTTCAGA
TACTCGACCATGGGACCCACTTAATGATATGATACAATTTGAAAAAGATGGAGTTATCCAGACCAAAGTG
AAACATCGCACTCCAATGGTTAGTGTTCCAAAAATGAAACATAAACCAACAAGGCAGCAGAAGAAAGTGG
TAAAAGGCTATTCTTCCCCAGAACCTGACATCCAGGATTCCTCTGGAAGTGAAGCTCAATCTGTAAAACC
AAGTACACGAAGGAAGAAGGGGATAGACCTGGGAGACATTCAGAGTTCCATTGAGTCTATAAAGCAGACA
CAGGAAGAGATTAAAAGAAATATTATGGCTCTTCGAAATCATTTACTTTCAAGTACACCTGCTACAGATT
ATTTTCTGCAACAGAAAGACTACTTCGTCATTTTCCTTCTGATTTTGCTTCAAGTCATAATAAACTTTAT
ATTTAAG

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG214742 representing NM_001003717
Red=Cloning site Green=Tags(s)

MEAALADGEPDRSSLLGDSKDVLGPSTVVANSDEPQHLTPGKMSQRQGRDANPTPTRDLPQPSLSPASLH
SQGFERGKEDISQNKDDSSLSMSKSKSESKLYNGSEKDSSTSSKLTKKESLKVQKKNYREEKKRATKELL
STITDPSVIVMADWLKIRGTLKSWTKLWCVLKPGVLLIYKTQKNGQWVGTVLLNACEIIERPSKKDGFCF
KLFHPLEQSIWAVKGPKGEAVGSITQPLPSSYLIIRATSESDGRCWMDALELALKCSSLLKRTMVREGKE
HDLSISSDSTHVTLYGLLRANNLHSGDNFQLNDSEIERQHFKDQDLYSDKSDKENDPEHDESDNEVLGKS
EESDTDTSERQDDSYIDPEPVEPLKETTYMEQSHEELGEAGEASQTETVSEENKSLIWTLLKQVRPGMDL
SRVVLPTFILEPRSFLDKLSDYYYHADFLSEAALEENPYFRLKKVVKWYLSGFYKKPKGLKKPYNPILGE
TFRCLWIHPRTNSKTFYIAEQVSHHPPISAFYVSNRKDGFCLSGSILAKSKFYGNSLSAILEGEARLTFL
NRGEDYVMTMPYAHCKGILYGTMTLELGGTVNITCQKTGYSAILEFKLKPFLGSSDYVNQISGKLKLGKE
VLATLEGHWDSEVFINDKKTDNSEIFWNPTPDIKQWRLIRHTVKFEEQDDFESEKLWQRVTKAINAKDQT
EATQEKYVLEEAQRQAARDRKTKTQEWVCKLFELDPLTGEWHYKFSDTRPWDPLNDMIQFEKDGVIQTKV
KHRTPMVSVPKMKHKPTRQQKKVVKGYSSPEPDIQDSSGSEAQSVKPSTRRKKGIDLGDIQSSIESIKQT
QEEIKRNIMALRNHLLSSTPATDYFLQQKDYFVIFLLILLQVIINFIFK

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001003717
ORF Size 2667 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001003717.1, NP_001003717.1
RefSeq Size 7115 bp
RefSeq ORF 2544 bp
Locus ID 237542
UniProt ID B9EJ86
Cytogenetics 10 D1
Summary This gene encodes a member of the oxysterol-binding protein (Osbp) family, a group of intracellular lipid receptors. Like most members, the encoded protein contains an N-terminal pleckstrin homology domain and a highly conserved C-terminal Osbp-like sterol-binding domain. Two transcript variants encoding different isoforms have been found for this gene. provided by RefSeq, Jul 2008
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Documents
Resources
"NM_001003717" in other vectors (4)
SKU Description Size Price
MC222080 Osbpl8 (untagged) - Mouse oxysterol binding protein-like 8 (Osbpl8), transcript variant 2, (10ug) 10 ug
$854.00
MR214742 Osbpl8 (Myc-DDK-tagged) - Mouse oxysterol binding protein-like 8 (Osbpl8), transcript variant 2 10 ug
$589.00 $776.00
MR214742L3 Lenti ORF clone of Osbpl8 (Myc-DDK-tagged) - Mouse oxysterol binding protein-like 8 (Osbpl8), transcript variant 2 10 ug
$1,076.00
MR214742L4 Lenti ORF clone of Osbpl8 (mGFP-tagged) - Mouse oxysterol binding protein-like 8 (Osbpl8), transcript variant 2 10 ug
$1,076.00

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