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Tmem229a (NM_177013) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG214729
Tmem229a (tGFP-tagged) - Mouse transmembrane protein 229A (Tmem229a), (10ug)
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ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $657.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Tmem229a
Synonyms 6332401O19Rik
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG214729 representing NM_177013
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGGGCAGCGATGTGGCGAGTGAGGGGCCATCACCGAGGGACGGCGCGACCCGGAGACCCGGGGCCA
CCGGCGGACTGCGTAGCCAGGCGGCCGCCAGCTGCCCTGAGCCGCTGTCCGCTGCTGAAGCTCCGGCCGA
GCGCGGCGCGCTGCCCGCCTGGATGCGCCTCTACTTCTACGGGATGCACGGCATCACCCTGGACGTGCTG
GTATCCTCTGCCAGGCGCTTCGCCCGCAGCCTGGATCTGCGGATGCTGGGTTTCTCTTCACCCTACCGCT
GCCTCCTGCACTCGCTCACCCACTTCGCCCTGGAGCAGCTCTACCTGCAGCGGCCGCGCTGCCCCAGCGC
CTTCCTCTTCAATTTCCTGCTTTACCCCTCTGCGCACGTCGGGCTGCAGACCCTGGCTGGCCAGGCGCTA
CGGCTCAGCCTAGGCGGCGGGCCGGGGGGCGCGGCAGCGCCGGCGCTGGGGGCGCTCGACCTGGCGCTGC
AGTACGTGCTCGCGCTCTACCACGGCCAAGTGTTCCTGAAGCGCTTCTTGTGTTTGCGGTACCCGCGGCG
GCGCGACCAGCACACCAGGGACACGCTACCCGCAGCCCGGGACGCCCAGATCCTTTGGGAGGCTGGTGGC
CAGCGACGAGGACCGGGTGGCGCAAGGGGCACAGAACGATCTCCTACCCAGGGACTGCCCGACCTCCTCC
GCTTCCTTTTCTTCGGAATGCATGGCTTTCTGGATGAGATCTTCTTCACCTTCTTCTTCAATGTGCTGGG
GCAGGGGGACAGGGCCAGCAGTGGCCACACATCCCTCTGGTCTTTCTTCATGTACGGAAGTTGTAGTTTC
GTGGTGGAAAAACTCTACTTCCACCTTCACTACAGCCGTGGCTGGGGCACGTGGAAGCGGGTGCCCATCT
ATGTGATCTTCATCTATGCGTGGGAGTTTTCCTGGGGTCTGGGACTTCGAATGTGCGGGGCCTGTTCCTG
GGACTATTCTCACTATCCTCTTAATTTCATGGGCCTCATCACCTTGATGTATTTACCTGGCTGGCTATTT
CTTAGTGTGTACCAGGACCTCCTGTCCAACGTGTTGTGGCGGGTGCAGTATGTCCCAACGAAC

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG214729 representing NM_177013
Red=Cloning site Green=Tags(s)

MAGSDVASEGPSPRDGATRRPGATGGLRSQAAASCPEPLSAAEAPAERGALPAWMRLYFYGMHGITLDVL
VSSARRFARSLDLRMLGFSSPYRCLLHSLTHFALEQLYLQRPRCPSAFLFNFLLYPSAHVGLQTLAGQAL
RLSLGGGPGGAAAPALGALDLALQYVLALYHGQVFLKRFLCLRYPRRRDQHTRDTLPAARDAQILWEAGG
QRRGPGGARGTERSPTQGLPDLLRFLFFGMHGFLDEIFFTFFFNVLGQGDRASSGHTSLWSFFMYGSCSF
VVEKLYFHLHYSRGWGTWKRVPIYVIFIYAWEFSWGLGLRMCGACSWDYSHYPLNFMGLITLMYLPGWLF
LSVYQDLLSNVLWRVQYVPTN

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_177013
ORF Size 1113 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_177013.3, NP_795987.2
RefSeq Size 4985 bp
RefSeq ORF 1116 bp
Locus ID 319832
UniProt ID B9EJI9
Cytogenetics 6 A3.1
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Documents
Resources
"NM_177013" in other vectors (4)
SKU Description Size Price
MC214430 Tmem229a (untagged) - Mouse transmembrane protein 229A (Tmem229a), (10ug) 10 ug
$503.00
MR214729 Tmem229a (Myc-DDK-tagged) - Mouse transmembrane protein 229A (Tmem229a) 10 ug
$289.00 $457.00
MR214729L3 Lenti ORF clone of Tmem229a (Myc-DDK-tagged) - Mouse transmembrane protein 229A (Tmem229a) 10 ug
$757.00
MR214729L4 Lenti ORF clone of Tmem229a (mGFP-tagged) - Mouse transmembrane protein 229A (Tmem229a) 10 ug
$757.00

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.