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Catsperd (NM_175350) Mouse Tagged ORF Clone

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SKU
MG214687
Catsperd (tGFP-tagged) - Mouse transmembrane protein 146 (Tmem146), (10ug)
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ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $937.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Catsperd
Synonyms 619718; 4921529N20Rik; 4933402B14Rik; AW045609; Gm6095; Tmem146
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG214687 representing NM_175350
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCTGGTGTTGATGCTAGCAGCAGCAGTGGCCACCATGGTCAGGGCTCACACACTGTGCCGGGTGCACA
CAGTGAGGACCGGGAAAGTGTTTAAGTCCAATATACAACTTCAAGGGGACCCACTGTTCTACGCCTTCCC
CAACACATTTGTGCTCAAGAACGTGTGCAAGGCTGACATTTCTGTGTATCTAGGGCAGAAAGTTTTCTTG
ACGATAGACAACTTTGAGTCCAGCCTCTTGCCGCTCACCGTTCCCAAGTCATTGGCGGTGGGGGTGCCAT
CAATCACAAGCGCCCACTTTGTCTCCGGCTCACTAGTACTGTTTGTGATCAGTGGAAAAGGCTACAGTTA
CGATTACTATGAGAACACCTGGCGTAAATTGGAAGGTATATCAGAACCTGTCTCTCACATTAGTGGTGAC
GTTTGTTGTTTCAAAGGCTCCTTTTGCCTGGAGTTATCAAATAACCTGTTTGCATATCTCCGTGGTGGCC
AGATCCCTGGGACCAACATATATTTCTCTGACAATGGAGGATTTTCATTCCAGCTCATGAATACTGACAA
ACTGAGTCACCTGACAGGAACACTAGGAGGCATCTTCCACTTACATTCCATGTCTCAGGTGGGGGTACTC
ATGGTTGAAAATAATTTGGGCACATTTCATTACATGGAGTACCCCCTGAACCATAGTATGGGGATAGCCT
TTTCATACAAGAATCTCCTTGAAGTCATTATGAAACCATACCAGAGGGGGTTTATGGTCCTCTGGAATCA
GAAAAGTATATTGGTGTCCTCCAATTCAGGCCAGATTGTTGAACACGTGCGGCTGATAGATCAGAAGATC
TTTACAGACTTAGATGTCGAACATGCCAACATCAACATCTACAGTGTTGCCTCAAATGCTTACGAGCTGG
CTTTTTTGGTAGCAGAAGATCATCTGTACTATGGAAGTCAGAGTTACATGGGAACCTATGTAATCAAACT
GCCCCATCAGCCTCTCTGGTCTACACACACAAGCATCTACTTTGAGGACATCGGCATACTGCAGGTCCTC
ACCCCTGTTGCTGACCCACACTTTGCAGCCTATGACTTCGACAAGTGTACAGTGAATGTGCAGTCCTCTC
TCATGGATGAAAAGCTGGCGCTTCAACCATGTAATGTGGAACTTCTGGAAAGCACCATGATAAATACAAT
GTTTACAATAGACATGAATAGCAAGCTGAAACTGTCTGCCCTCATGATACCCCGGAAGGGCGAGAACCCT
ACCCCTCTGGTCATGGTCAGCAATCCCCATGCCCTGGGGTTTAAGGCAAACCTCAATGAGTTTGGCAATA
CGTTTGATGGCAACTCTAAGTACAAACTGGATATTGAACTCAAACAGCAGCATCACTGGGGCAACTCAGA
TTTCAACTTCACTGCCAGCATCAAGCGCCATGCCATCTCCTCTGTAACCGTGGACATAGCTGACAAGACA
CTTTCGTGTGTGGACCTAAAGCCTCTGTCCACGCTCATCTCAGTTGGCTGTGACATGACAAAGAAAATTG
TTGTGCAAAACAAGATCTCCGCCTGCACCATGGGAATCCTGAACCCCGTGCAGCTGCAGAAGAACTATAC
TTACACCATTGAGAAGGAAGCATACGATCCTATCAACCACAATGGTGAAGCCCAGGATGACCTGATAGTA
TTTTACGAGTACAAGGACCTGGGCTGCCCGCGCCTAGTCTACTATGACAAGCCGTGGAAACCTGTGGTGG
AACTGTGGAAGAATGGGATTGTAGAAGAAATCATGAACGCAGAGTATGTCATCTCGGAGATAAATGGACT
AGTCACCTATTCATACTCCTTGACGGCTGCCACTGCAAACTGCCGGTCACAGCCCCAGAACTGGAGCACC
TTTGAATCGGATATTGAGAACGAAGAACCGTTCCTCTGGAACAGAGAGAACTATGTCAGCTGCCACGAAG
ACAACAAGGACAACCCCCTGCTATGGCCGAATGTCGAGTATCAGGTCTTAGGTGGCCAGACAAACAACAA
GATTATTTTTGGCCAAAGAAATGGGATCTATACCTTCCATCTGTCTGTGGTAGATCCATACTACAGCTAC
TGCAACCTGAACACCATATTCAGCGTGTACGTGCACGGGGCCTTACCGGTGACGAAGTTCCAACCATTGC
TCACCATCCTCCTGATGGTCACCACAACGCTGCTGACTGCGTGGCTGGCCTATGCTATCCCCAAACAGCT
GAGGAGTGAGAAAGGCCAGCGACTTCTGGGTTTCTGCTACCAGATACTCCAGCTCTGCCTAGGGGTCTGC
TTCTGCACCTGGCTGCGTGGCAAACTGAGACAGTGGCTGCGCCCCAGAAGGGTGAAAGACCAGAACAGAG
GCAAGGTGCGAGTTGCCCAGAAACATCCAGAGACA

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG214687 representing NM_175350
Red=Cloning site Green=Tags(s)

MLVLMLAAAVATMVRAHTLCRVHTVRTGKVFKSNIQLQGDPLFYAFPNTFVLKNVCKADISVYLGQKVFL
TIDNFESSLLPLTVPKSLAVGVPSITSAHFVSGSLVLFVISGKGYSYDYYENTWRKLEGISEPVSHISGD
VCCFKGSFCLELSNNLFAYLRGGQIPGTNIYFSDNGGFSFQLMNTDKLSHLTGTLGGIFHLHSMSQVGVL
MVENNLGTFHYMEYPLNHSMGIAFSYKNLLEVIMKPYQRGFMVLWNQKSILVSSNSGQIVEHVRLIDQKI
FTDLDVEHANINIYSVASNAYELAFLVAEDHLYYGSQSYMGTYVIKLPHQPLWSTHTSIYFEDIGILQVL
TPVADPHFAAYDFDKCTVNVQSSLMDEKLALQPCNVELLESTMINTMFTIDMNSKLKLSALMIPRKGENP
TPLVMVSNPHALGFKANLNEFGNTFDGNSKYKLDIELKQQHHWGNSDFNFTASIKRHAISSVTVDIADKT
LSCVDLKPLSTLISVGCDMTKKIVVQNKISACTMGILNPVQLQKNYTYTIEKEAYDPINHNGEAQDDLIV
FYEYKDLGCPRLVYYDKPWKPVVELWKNGIVEEIMNAEYVISEINGLVTYSYSLTAATANCRSQPQNWST
FESDIENEEPFLWNRENYVSCHEDNKDNPLLWPNVEYQVLGGQTNNKIIFGQRNGIYTFHLSVVDPYYSY
CNLNTIFSVYVHGALPVTKFQPLLTILLMVTTTLLTAWLAYAIPKQLRSEKGQRLLGFCYQILQLCLGVC
FCTWLRGKLRQWLRPRRVKDQNRGKVRVAQKHPET

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_175350
ORF Size 2415 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_175350.3, NP_780559.2
RefSeq Size 2565 bp
RefSeq ORF 2418 bp
Locus ID 106757
UniProt ID E9Q9F6
Cytogenetics 17 D
Summary Auxiliary component of the CatSper complex, a complex involved in sperm cell hyperactivation. Sperm cell hyperactivation is needed for sperm motility which is essential late in the preparation of sperm for fertilization. Required for CATSPER1 stability before intraflagellar transport and/or incorporation of the CatSper complex channel into the flagellar membrane.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_175350" in other vectors (4)
SKU Description Size Price
MC221772 Catsperd (untagged) - Mouse transmembrane protein 146 (Tmem146), (10ug) 10 ug
$812.00
MR214687 Catsperd (Myc-DDK-tagged) - Mouse transmembrane protein 146 (Tmem146) 10 ug
$589.00 $737.00
MR214687L3 Lenti ORF clone of Catsperd (Myc-DDK-tagged) - Mouse transmembrane protein 146 (Tmem146) 10 ug
$1,037.00
MR214687L4 Lenti ORF clone of Catsperd (mGFP-tagged) - Mouse transmembrane protein 146 (Tmem146) 10 ug
$1,037.00

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.