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Rnf148 (NM_027754) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG214589
Rnf148 (tGFP-tagged) - Mouse ring finger protein 148 (Rnf148), (10ug)
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ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $500.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Rnf148
Synonyms 4933432M07Rik; Greul3
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG214589 representing NM_027754
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTTACTTTGTGTTTCATGCCTATCAGTTAATGGAGAAATGAACCCACTTGGACCTACTCCTTCAGTTC
ATAGGTCTGTTTCATTTTGGCTGTTGAGACTTAGCGTCTTCCTACTGCTTAGCCTCCGTGACTCCAAAGG
AAAAGCAATATGGACTGCTCATCTGAATATTACATTCCAGGTGGGAAACCGGATCATATCAGAGCTAGGA
GAGAGTGGAGTGTTTGGGAATCACTCTCCTCTGGAAAGGGTGTCTGGTGCCGTGGTACTTCCTGAAGGAT
GGAATCAGAATGCTTGCAGTCCGCTGACCAACTTCAGCAGGCCCGATCAGACAGACACTTGGCTGGCCCT
CATCGAAAGGGGAGGCTGCACTTTCACTCATAAAATCAACCTGGCAGCGGAGAAGGGAGCAAACGGGGTG
ATCATCTATAACTATCCGGGTACAGGCAACAAGGTGTTTCCCATGTCTCACCAGGGAACAGAGAATATAG
TTGCAGTGATGATTGGCAACCTCAAGGGCATGGAGCTCTTGCACTTGATCCAGCAAGGTGTCTATGTGAC
GATCATCATTGAAGTGGGGAGAATGCATATGCCGTGGCTCAGCCACTATGTTATGTCTCTATTTACCTTC
CTGGCAGCCACCGTCACCTACCTTTTCCTATACTGTGCCTGGAGACCCCGAGTGTCCAATTCTTCTACCA
GGAGGCAAAGGCAGCTAAAGGCCGATGTGAAGAAAGCTATTGGCCAGCTGCAACTTCGAGTGCTCCAGGA
CGGAGATAAGGAGTTAGACCCGAATGAAGACAGCTGCGTGGTTTGTTTTGACATGTACAAAGCCCAAGAT
GTAATACGTATTTTAACTTGCAAACATTTCTTCCACAAGACTTGCATCGATCCCTGGCTTTTAGCACATA
GGACATGCCCCATGTGCAAGTGTGACATCCTGAAACCT

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG214589 representing NM_027754
Red=Cloning site Green=Tags(s)

MLLCVSCLSVNGEMNPLGPTPSVHRSVSFWLLRLSVFLLLSLRDSKGKAIWTAHLNITFQVGNRIISELG
ESGVFGNHSPLERVSGAVVLPEGWNQNACSPLTNFSRPDQTDTWLALIERGGCTFTHKINLAAEKGANGV
IIYNYPGTGNKVFPMSHQGTENIVAVMIGNLKGMELLHLIQQGVYVTIIIEVGRMHMPWLSHYVMSLFTF
LAATVTYLFLYCAWRPRVSNSSTRRQRQLKADVKKAIGQLQLRVLQDGDKELDPNEDSCVVCFDMYKAQD
VIRILTCKHFFHKTCIDPWLLAHRTCPMCKCDILKP

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_027754
ORF Size 948 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_027754.1, NP_082030.1
RefSeq Size 1242 bp
RefSeq ORF 951 bp
Locus ID 71300
UniProt ID G3X9R7
Cytogenetics 6 A3.1
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Documents
Resources
"NM_027754" in other vectors (4)
SKU Description Size Price
MC211170 Rnf148 (untagged) - Mouse ring finger protein 148 (Rnf148), (10ug) 10 ug
$330.00
MR214589 Rnf148 (Myc-DDK-tagged) - Mouse ring finger protein 148 (Rnf148) 10 ug
$289.00 $300.00
MR214589L3 Lenti ORF clone of Rnf148 (Myc-DDK-tagged) - Mouse ring finger protein 148 (Rnf148) 10 ug
$600.00
MR214589L4 Lenti ORF clone of Rnf148 (mGFP-tagged) - Mouse ring finger protein 148 (Rnf148) 10 ug
$600.00

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.