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Olfr1447 (NM_146703) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG214465
Olfr1447 (tGFP-tagged) - Mouse olfactory receptor 1447 (Olfr1447), (10ug)
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ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $500.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Olfr1447
Synonyms MOR202-3
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG214465 representing NM_146703
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGACAATAATGAAGAACAGGACAGAAGTGACAGAGTTCATTCTGCTGGGGCTCACCAATGACCCAGGCC
TGCAGCTTCCCCTGTTTATCACCTTCCTTCTCATCTACACCATCACCGTGGTGGGAAACCTAGGGATGAT
CCTGCTGATTGTCCTGGACTCTCGGCTCCACACCCCCATGTACATTTTCCTTGGTAATCTTTCTCTGGTG
GATTTCTGTTACTCTACAGCTGTCACACCCACAGTCATGAATGGGCTACTAATAGGAAACAAGGTCATTT
CCTACAATGATTGTGCTGCTCAGATGTTCTTTTTTGGAGCCTTTGCTACTGTGGAGAACTATCTGTTGGC
CTCAATGGCCTATGATCGCTATGCAGCAGTGTGTAAGCCCCTACACTATGCCACCATCATGACTAAAAGT
ATGTATACATGGCTTATCACTGGCTCTTATGTCATCAGTTTTACAAATTCCTCCATCCATATTGCAGACA
TATTCACTCTCTCCTTCTGCAAGTCCAATGTGATCCATCATTTTTTCTGTGATGTTCCTGCAATCATGGC
TCTCACTTGTTTTGATAATCAAGTTAGGGAACATGTTCTTCTTTACATTGAGAGTTTAAATGTGTTTTTT
GCTCTAATAGTTATTTGTACATCTTACATGTTAATTTTTGTCACCATCTTAAAGATGCACTCAGCTTCAG
GACATCACAAGGCTATATCCACCTGTGCCTCTCACTTCACTGCAGTGTCCATTTTCTATGGAACTGTCAT
TTTCATGTATTTACAGCCCAGCTCCAATCATTCCATGGACACAGACAAAGTAACATCTGTATTCTACACC
ATGGTCATTCCTATGCTGAATCCTCTAGTCTACAGCCTGAGGAACAAGGAGGTGAAGAATGCATTTATAA
AATTGATACTACATTAT

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG214465 representing NM_146703
Red=Cloning site Green=Tags(s)

MTIMKNRTEVTEFILLGLTNDPGLQLPLFITFLLIYTITVVGNLGMILLIVLDSRLHTPMYIFLGNLSLV
DFCYSTAVTPTVMNGLLIGNKVISYNDCAAQMFFFGAFATVENYLLASMAYDRYAAVCKPLHYATIMTKS
MYTWLITGSYVISFTNSSIHIADIFTLSFCKSNVIHHFFCDVPAIMALTCFDNQVREHVLLYIESLNVFF
ALIVICTSYMLIFVTILKMHSASGHHKAISTCASHFTAVSIFYGTVIFMYLQPSSNHSMDTDKVTSVFYT
MVIPMLNPLVYSLRNKEVKNAFIKLILHY

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_146703
ORF Size 927 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_146703.1, NP_666914.1
RefSeq Size 930 bp
RefSeq ORF 930 bp
Locus ID 258698
Cytogenetics 19 A
Summary Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. provided by RefSeq, Jul 2008
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Documents
Resources
"NM_146703" in other vectors (4)
SKU Description Size Price
MC213852 Olfr1447 (untagged) - Mouse olfactory receptor 1447 (Olfr1447), (10ug) 10 ug
$330.00
MR214465 Olfr1447 (Myc-DDK-tagged) - Mouse olfactory receptor 1447 (Olfr1447) 10 ug
$289.00 $300.00
MR214465L3 Lenti ORF clone of Olfr1447 (Myc-DDK-tagged) - Mouse olfactory receptor 1447 (Olfr1447) 10 ug
$600.00
MR214465L4 Lenti ORF clone of Olfr1447 (mGFP-tagged) - Mouse olfactory receptor 1447 (Olfr1447) 10 ug
$600.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.