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Olfr825 (NM_146677) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG214423
Olfr825 (tGFP-tagged) - Mouse olfactory receptor 825 (Olfr825), (10ug)
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ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $500.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Olfr825
Synonyms MOR210-1
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG214423 representing NM_146677
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGCGACAGGGAAACAAGCAATCATTCAGATATGACTGACTTTATCCTTGTAGGCTTCAGGGTCAGTC
CTGAGCTCCATATCCTCCTTTTCCTGCTCTTTCTGCTTGTGTATGCCATGATCCTTCTAGGAAACCTTGG
GATGATGGCCATCATTATGACTGATCCCAGGCTGAACACACCAATGTATTTCTTCCTAGGCAACCTCTCC
TTCATCGACCTCTTCTACTCATCTGTTATTGCACCAAAGGCTATGAGCAACTTTTGGACAGAGAGCAAGT
CTATCTCATTTGCAGGCTGTGTGGCCCAGATCTTTCTCTTTGCACTGTTCATTGTGGCTGAAGGATTTCT
CCTGGCAGCCATGGCTTATGACCGTTTCATTGCCATCTGCAACCCACTCCTGTATTCTGTTCACATGTCC
ACACGTCTCTGCACTCAGCTGGTGGCAGGCTCTTATTTTTGTGGCTGTATCAGCTCTGTTCTCCAGACTA
GCATGACATTTACTTTATCCTTTTGTGCTTCTCGAGCCATTGACCACTTTTACTGTGATAGTCGGCCACT
TCAGAGACTCTCCTGTTCTGACATTTTCATACACAAAATAGTATCTTTTTCTTTATCTGGCATCATTATC
TTACCAACTATCACAGTCATCATTGTTTCCTACATGTATATTGTGTCCACTGTTCTAAAGATACGATCCG
TGGAGGGAAGGAAGAAAGCCTTCTCCACTTGCAGCTCTCACCTGGGTGTCGTGAGTGTGCTGTATGGTGC
TGTTTTCTTCATGTATCTCACCCCTGACAGATTTCCTGAACTGAGTAAGCTGGCTTCTTTGTGCTATTCC
CTAGTCACACCTATGCTGAACCCTCTGATTTACTCTCTGAGAAACAAAGATGTTAGAGATGCTTTAAGCA
AACTTCTAGAGAAGAAAAAATGCAGTGGTTCGTTTTTCCCATTCTACAAG

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG214423 representing NM_146677
Red=Cloning site Green=Tags(s)

MGDRETSNHSDMTDFILVGFRVSPELHILLFLLFLLVYAMILLGNLGMMAIIMTDPRLNTPMYFFLGNLS
FIDLFYSSVIAPKAMSNFWTESKSISFAGCVAQIFLFALFIVAEGFLLAAMAYDRFIAICNPLLYSVHMS
TRLCTQLVAGSYFCGCISSVLQTSMTFTLSFCASRAIDHFYCDSRPLQRLSCSDIFIHKIVSFSLSGIII
LPTITVIIVSYMYIVSTVLKIRSVEGRKKAFSTCSSHLGVVSVLYGAVFFMYLTPDRFPELSKLASLCYS
LVTPMLNPLIYSLRNKDVRDALSKLLEKKKCSGSFFPFYK

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_146677
ORF Size 960 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_146677.1, NP_666888.1
RefSeq Size 963 bp
RefSeq ORF 963 bp
Locus ID 258672
Cytogenetics 10 D3
Summary Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. provided by RefSeq, Jul 2008
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Documents
Resources
"NM_146677" in other vectors (4)
SKU Description Size Price
MC213827 Olfr825 (untagged) - Mouse olfactory receptor 825 (Olfr825), (10ug) 10 ug
$330.00
MR214423 Olfr825 (Myc-DDK-tagged) - Mouse olfactory receptor 825 (Olfr825) 10 ug
$289.00 $300.00
MR214423L3 Lenti ORF clone of Olfr825 (Myc-DDK-tagged) - Mouse olfactory receptor 825 (Olfr825) 10 ug
$600.00
MR214423L4 Lenti ORF clone of Olfr825 (mGFP-tagged) - Mouse olfactory receptor 825 (Olfr825) 10 ug
$600.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.