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Pcdhb10 (NM_053135) Mouse Tagged ORF Clone

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SKU
MG214414
Pcdhb10 (tGFP-tagged) - Mouse protocadherin beta 10 (Pcdhb10), (10ug)
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ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $930.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Pcdhb10
Synonyms Pcdhb5D; PcdhbJ
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG214414 representing NM_053135
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGATGACACTAGCAAAAACGCCAGAGAACAGGCAAGTCCTTTTTTTTACTATACTATTGCTATTAT
GGAAGTCTTGTGCCGATGCAATTATATATTCTATACCAGAAGAAACAGAGAGTGGCTATTTGGTGGCTAA
TTTGGCAAAAGATCTGGGGCTCCGAGTTGGAGAGCTGGCTATTAGGGGGGCACGAATCCATTACAAAGGA
AACAAAGAGCTCTTGCAGCTGGATGCAGAGACTGGGAATTTATTCCTGAAGGAAAAACCAGACCGTGAGG
TGCTGTGCAGGGCGACAGAACCCTGTGTGCTGCACTTCCAGATCATACTGGAAAACCCTGTGCAGTTCTT
CCAAACTGAACTGCAGCTCACAGATATAAATGACCACTCTCCAGAGTTCCCTGACCGGAAAATGCTACTG
AAAATCCCTGAGAGCGCCCAGCCAGGGACAACGTTTCCTCTGAAGGCAGCCCAGGACCCTGACATAGGGA
GCAATGCTGTTCAGAACTACACAGTCAGTCCCAACCTCCATTTCCATGTGGTTACTCTCAGTCGCTCAGA
TGGCAGGAAATACCCAGAGCTGGTGCTGGACAGAGCCCTGGACAGGGAGGAGCAGCCTGAACTCACTTTA
ATCCTCACTGCTCTGGATGGTGGAGCTCCACCCAGGTCAGGAACCACCGAAGTTCACATTGAAGTCGTGG
ACATCAATGATAACGCCCCCCAGTTTATACAGTCACTCTATGAGGTGCAGATTCTTGAAAATAGCCCCCT
TGATGCCTTAGTTGCCACAGTCTCTGCCAGGGATTTAGATGCTGGGATATATGGCAAGGTAGCCTACTCT
CTGTTTCAGGATGATGGAATATCTCAACCATTTGTAATAGATGAAATCACAGGAGAAATTCGTCTTAAAG
GGGCACTGGATTTTGAGGCAACTCCGTATTATAACATGGAAATTGTAGCTACAGACCCAGGAGGCTTTTC
AGGAAAATGCACTGTGGTTATCCAGGTGGTGGATGTGAATGACAATGCCCCTAAGCTCACCATATCTTCA
CTCAATAGTTCTATCCCAGAAAATGCTCCTGAGACTGTAGTGGCTGTTTTCAGTGTTTCTGATCCGGATT
CTGGGGAAAATGGAAGGATGGTGTGTTCTATTCAGAACGAACTTCCATTTCTTTTGAAGTCCACATTTGA
GAACTATTATACTTTAGTAACAGAGGGGACACTTGATAGAGAGAGCAAAGCTGAATACAACATTACTATC
TCCGTCACCGACCTGGGCACCCCCAGGCTCACAACCCAGCACACCATAACAGTGCAGGTGTCTGACATCA
ACGATAATGCCCCCACCTTCACCCGAACCTCCTACACCATGTTCATCCGGGAGAACAACAGCCCTGCCCT
GCACATTGGCACCATCAGCGCCACAGACTCAGACGCAGGCTCCAATGCCCACATCACATACTCGCTGCTG
CCGCCCCATGATCCGCAGCTGACCCTCACCTCTCTTGTCTCCATCAATGAAGACAATGGGCAGCTGTTCG
CACTCAGGGCTCTGGACTATGAGGCCCTGCGGGCCTTCGAGTTTCATGTTCGCGCCACAGACCGTGGTTC
ACCCGCGCTTAGCAGCCAGGCTTTGGTACTCGTGGTGGTGCTGGACGACAATGACAATGAGCCCTTCGTG
CTCTACCCGTTGCAGAACTCTTCTGCGCCCTGTAAGGAGCTAGTACCCAGAGCTGCGGAGGCAGGTTACT
TAGTCACCAAGGTGGTGGCTGTGGACCGCGACTCTGGCCAGAATGCCTGGCTGTCATTCCAGTTACTCAA
GGCCACGGAGCCCGGGCTGTTCAGCGTGTGGGCGCACAATGGGGAGGTGCGCACCACCAGGCTGTTGAGT
GAGCGAGATGCACCCAAGCACAGGCTGCTGCTGGTGGTCAAGGACAATGGGGATCCTCCGCGCTCTGCCA
GTGTCACACTGCATGTGTTGCTGGTGGATGGCTTCTCTCAGCCCTACCTGCCTTTGTCTGAAGTCGTGCA
GGATCCCGCTCAGGAAGGTGATGACGCTCTCACGCTGTACTTGGTCATTGCTTTGGCATCTGTGTCTTCA
CTTTTCCTCATTTCTGTTCTGCTCTTCGTTGGGATGAAGCTGTGCAAGAGGGCCAGGTCGCCTTCTCTGG
GTGTCTGCTCTGTGCCTGAGGGCCACTTTCCTGGCCACCTGGTGGATGTTAGCGGAACTGGGACTCTGTC
CCAGAGCTACCAGTATGAGGTGTGTTTATCGGGGGACTCTGGGACTGGTGAGTTCAAATTCCTTAAACCC
ATGATCTCCAACTTAATGGTTCAGGACCCTGAGAGAGAAGTGAAGGAAAGTCCGCACTGCAGGGATAGCT
TTGTATTCAGT

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG214414 representing NM_053135
Red=Cloning site Green=Tags(s)

MEMTLAKTPENRQVLFFTILLLLWKSCADAIIYSIPEETESGYLVANLAKDLGLRVGELAIRGARIHYKG
NKELLQLDAETGNLFLKEKPDREVLCRATEPCVLHFQIILENPVQFFQTELQLTDINDHSPEFPDRKMLL
KIPESAQPGTTFPLKAAQDPDIGSNAVQNYTVSPNLHFHVVTLSRSDGRKYPELVLDRALDREEQPELTL
ILTALDGGAPPRSGTTEVHIEVVDINDNAPQFIQSLYEVQILENSPLDALVATVSARDLDAGIYGKVAYS
LFQDDGISQPFVIDEITGEIRLKGALDFEATPYYNMEIVATDPGGFSGKCTVVIQVVDVNDNAPKLTISS
LNSSIPENAPETVVAVFSVSDPDSGENGRMVCSIQNELPFLLKSTFENYYTLVTEGTLDRESKAEYNITI
SVTDLGTPRLTTQHTITVQVSDINDNAPTFTRTSYTMFIRENNSPALHIGTISATDSDAGSNAHITYSLL
PPHDPQLTLTSLVSINEDNGQLFALRALDYEALRAFEFHVRATDRGSPALSSQALVLVVVLDDNDNEPFV
LYPLQNSSAPCKELVPRAAEAGYLVTKVVAVDRDSGQNAWLSFQLLKATEPGLFSVWAHNGEVRTTRLLS
ERDAPKHRLLLVVKDNGDPPRSASVTLHVLLVDGFSQPYLPLSEVVQDPAQEGDDALTLYLVIALASVSS
LFLISVLLFVGMKLCKRARSPSLGVCSVPEGHFPGHLVDVSGTGTLSQSYQYEVCLSGDSGTGEFKFLKP
MISNLMVQDPEREVKESPHCRDSFVFS

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_053135
ORF Size 2391 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_053135.2, NP_444365.1
RefSeq Size 2842 bp
RefSeq ORF 2394 bp
Locus ID 93881
Cytogenetics 18 B3
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Documents
Resources
"NM_053135" in other vectors (4)
SKU Description Size Price
MC221716 Pcdhb10 (untagged) - Mouse protocadherin beta 10 (Pcdhb10), (10ug) 10 ug
$804.00
MR214414 Pcdhb10 (Myc-DDK-tagged) - Mouse protocadherin beta 10 (Pcdhb10) 10 ug
$589.00 $730.00
MR214414L3 Lenti ORF clone of Pcdhb10 (Myc-DDK-tagged) - Mouse protocadherin beta 10 (Pcdhb10) 10 ug
$1,030.00
MR214414L4 Lenti ORF clone of Pcdhb10 (mGFP-tagged) - Mouse protocadherin beta 10 (Pcdhb10) 10 ug
$1,030.00

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.