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Dennd6b (NM_027081) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG214375
Dennd6b (tGFP-tagged) - Mouse family with sequence similarity 116 member B (Fam116b), (10ug)
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ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $745.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Dennd6b
Synonyms 1700027J05Rik; AI414881; Fam116b
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG214375 representing NM_027081
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGGTGCCGGTGGGTCCCGGGCCCCGGCAAGCTGGCGGAGGACTAGGCGCGACAAGATCCTCGTCCT
CCGGCCGCGCGGCGCGGACCGCGGAGATGCCCTGGGCGCGCTTCTCTGCCTGGCTGGAGTGTGTGTGCGT
GGTCACCTTCGATCTGGAGCTGGGCCAGGCGCTAGAGCTAGTCTACCCAAGTGACTTCCGGCTCACAGAC
AAGGAGAAAAGCAGCATCTGCTACCTGGCCTTCCCAGACTCGCACTCAGGGTGCCTGGGAGACACTCAGT
TCAGTTTCCGTATGCGTCAGTGTGGGGGACAAAGGAGCCTCTGGCAGGTGGATGATCGGTCGTATAACAA
CAAGGCCCCCTTGGCATTGCAGAGGGAGCCAGCACACTATCTGGGCTATGTGTACTTCAGACAGGTGAAG
GACAGCTCTGTGAAGAGGGGTTACTTCCAGAAGTCTTTAGTGCTGGTGTCCCGCCTGCCATTCGTCCGGC
TGTTTCAGTCACTCCTAAGCCTGATTGCCCCTGAATACTTTGAGAAGTTGGCACCCTGCCTGGAAGCAGT
TTGTAATGAGATTGACCAATGGCCAGCGCCGGTGCCTGGGCAGACCCTGAACCTACCCATCATGGGAGTC
GTCATTCAGGTGCGCATCCCATCCAGGGTGGACAAGCTGGAATCCAGTCCTCCAAAGCAGTGTGATCAAG
AGAACCTGCTGCCAGCTCCAGTTGTCCTCACTAGTGTCCATGAACTGGATCTGTTTAGGTGCTTCCGGCC
AGTGCTCACCCACGTGCAGACCCTGTGGGAACTCATGCTTCTTGGGGAGCCCCTCGTGGTGCTGGCACCC
TCACCCGACGTGTCTTCAGAGCTGGTGCTAGCCCTAACCAGCTGCCTCCAGCCCCTGAAGTTCTGCTGTG
ACTTCCGCCCCTACTTCACCATTCATGACAGTGAGTTCAAGGAGCTTACAACTCGAACCCAGGCTCCACC
AAACGTGGTCCTGGGAGTCACAAACCCTTTCTTTATCAAAACACTCCAGCACTGGCCTCATGTCCTCCGA
ATTGGGGAGCCCAAGATGTCAGGGGACCTTCCAAAGCAGGTCAAGCTTAAAAAACCCTCACGGCTGAAGA
CCCTTGACACCAAGCCAGGCCTCTACACCTCCTATACTGCCCACCTCCACCGGGACAAAGCGCTGCTTAA
ACGACTGCTCAAGGGCGTACAGAAGAAGCGGCCATGGGATGTGCAGAGTGCCCTGCTGAGGCGGCACCTT
CTAGAGCTCACACAGAGTTTCATCATTCCTCTGGAGCACTACATGGCCAGCCTCATGCCGCTACAGAAGA
ACATCACACCCTGGAAGAGCCCCCCGCAGATCTGCCCCTTCCGCCAGGACGATTTCCTGCGAAGCCTGGA
GCATGCGGGACCCCAGCTCACCTGCATCCTCAAGGGTGACTGGCTGGGCCTCTACAGGCGGTTTTTCAAG
TCCCCCCATTTTGATGGCTGGTATCGGCAACGACACAAAGAGATGGCCCAGAAGCTGGAAGCTCTGCACC
TGGAAGCTATTTGTGAGGCGAACATTGAGGCTTGGATGAAGGACAAGTCCGAGGTGGAAGTGGTCGACCT
GGTCCTGAAACTTCGGGAGAAGCTGGTACGGGCACAGGGCCACCAACTCCCCGTGAAGGAGGTGACACTG
CAGCGGGCTCAGCTGTACATTGATACAGTTATTGGCTCCCTTCCCAAGGACCTGCAAGCAGTCCTGTGCC
CTCCC

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG214375 representing NM_027081
Red=Cloning site Green=Tags(s)

MEVPVGPGPRQAGGGLGATRSSSSGRAARTAEMPWARFSAWLECVCVVTFDLELGQALELVYPSDFRLTD
KEKSSICYLAFPDSHSGCLGDTQFSFRMRQCGGQRSLWQVDDRSYNNKAPLALQREPAHYLGYVYFRQVK
DSSVKRGYFQKSLVLVSRLPFVRLFQSLLSLIAPEYFEKLAPCLEAVCNEIDQWPAPVPGQTLNLPIMGV
VIQVRIPSRVDKLESSPPKQCDQENLLPAPVVLTSVHELDLFRCFRPVLTHVQTLWELMLLGEPLVVLAP
SPDVSSELVLALTSCLQPLKFCCDFRPYFTIHDSEFKELTTRTQAPPNVVLGVTNPFFIKTLQHWPHVLR
IGEPKMSGDLPKQVKLKKPSRLKTLDTKPGLYTSYTAHLHRDKALLKRLLKGVQKKRPWDVQSALLRRHL
LELTQSFIIPLEHYMASLMPLQKNITPWKSPPQICPFRQDDFLRSLEHAGPQLTCILKGDWLGLYRRFFK
SPHFDGWYRQRHKEMAQKLEALHLEAICEANIEAWMKDKSEVEVVDLVLKLREKLVRAQGHQLPVKEVTL
QRAQLYIDTVIGSLPKDLQAVLCPP

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_027081
ORF Size 1755 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_027081.3, NP_081357.2
RefSeq Size 4716 bp
RefSeq ORF 1758 bp
Locus ID 69440
UniProt ID Q9D9V7
Cytogenetics 15 E3
Summary Guanine nucleotide exchange factor (GEF) for RAB14. Also has some, lesser GEF activity towards RAB35 (By similarity).UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_027081" in other vectors (4)
SKU Description Size Price
MC219309 Dennd6b (untagged) - Mouse family with sequence similarity 116, member B (Fam116b), (10ug) 10 ug
$600.00
MR214375 Dennd6b (Myc-DDK-tagged) - Mouse family with sequence similarity 116, member B (Fam116b) 10 ug
$289.00 $545.00
MR214375L3 Lenti ORF clone of Dennd6b (Myc-DDK-tagged) - Mouse family with sequence similarity 116, member B (Fam116b) 10 ug
$845.00
MR214375L4 Lenti ORF clone of Dennd6b (mGFP-tagged) - Mouse family with sequence similarity 116, member B (Fam116b) 10 ug
$845.00

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.