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Ankrd34a (NM_001024851) Mouse Tagged ORF Clone

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SKU
MG214346
Ankrd34a (tGFP-tagged) - Mouse ankyrin repeat domain 34A (Ankrd34a), (10ug)
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ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $657.00
3 Weeks*
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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Ankrd34a
Synonyms Ankrd34; AW060394
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG214346 representing NM_001024851
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCTGCACACCGAGGGCCACGCTCTTCTTCGAGCTGTGGGACAGGGTAAGCTACGCTTGGCCCGTTTGC
TTCTGGAGGGAGGAGCCTACGTGAATGAGGGTGATGCCCAGGGGGAGACTGCGCTAATGGCGGCCTGTAG
GGCCCGTTACGACGACCCTCAGAACAAGGCGCGCATGGTACGCTATCTCTTGGAGCAAGGCGCGGATCCC
AACATCGCAGACCGTTTAGGACGTACCGCGCTGATGCATGCTTGCGCCGGGGGTGGGGGAGCCGCAGTGG
CCTCTCTGCTCCTTGCCCACGGCGCAGACCCTTCAGTTCGAGATCACGCTGGCGCCTCGGCGCTTGTCCA
CGCCCTGGACCGAGGGGACCGCGAGACCCTTGCCACACTGCTGGACGCCTGCAAGGCCAAGGGCACGGAG
GTCATCATCATAACGACGGATACCTCGCCCTCGGGCACCAAGAAGACCAGGCAGTATCTTAATTCCCCAC
CGTCCCCAGGGGTAGAGGACCCTGCACCCGCTCCTCCTAGTCCAGGGGTCTGCACATCGCCTTCAGAAGT
CCAACTGCAGACTGCAGGAGGAGGGCGCGGGTTGTTATCCCCTCGCGCCCAGGAAGAAGAGGAGAAAAGA
GACATTTTTGAATTTCCTCTTCCCAAGCCCCCTGATGACCCTTCCCCTTCCGAGCCTCTCCCCAAGCCAC
CTCGACATCCTCCAAAACCACTCAAAAGGCTCAATTCCGAGCCCTGGGGCCTTGTGGCTCCTCCTCAACC
GGTTCCTCCTGCAGAAGGGAGGCCCGGTTTGGAGCGTCTGGCTGCTGAATTCAATGGCTTGACCCTGACC
GGGAGACCGCGTCTTTCTCGGCGTCACAGCACCGAAGGCCCGGAGGACCCGCCCCCATGGGCGGAGAAAG
TGACGGGTGGGGGTCCTCTCTCGCGCCGAAACACTGCGCCCGAGGCTCAGGAGTCTGGTCTTCCTTCAGG
GCTAAGGCAAAAACTGAGCCGCATGGAGCCGGTGGAGCTCGACACCCCTGGACATTTTTGCCCTGACTCC
CCCGAGTCCAGCCGGTTATCCCTGGAGCGCCGGCGATACAGCGCCTCTCCGCTGACCCTCCCTCCAGCGG
GCTCAGTTTCCTCCCCACGCCAGTCCCAGGAAAGTCTGCCCGGGGCTGTATCCCCGCTGAGCGGACGGAG
GCGGAGTCCAGGGCTGCTAGAGAGAAGGGGCTCCGGGACCTTGCTCCTGGACCACATCTCGCAAACACGA
CCAGGTTTCTTGCCTCCTCTCAACGTCAGTCCCCACCCTCCCATCCCCGACATTCGTCCCCAACCCGGAG
GTCGGGCACCCTCACTGCCTGCCCCTCCACCCTCTGGGGCACCAGGGTCTCCCAGGACCAAGCGCAAGTT
GGTGAGACGCCACTCCATGCAGACTGAGCAGATTCGCCTGCTAGGGGGGTTTCAGAGTCTAGGTGGGCCT
GGGGAACCCGGGCGC

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG214346 representing NM_001024851
Red=Cloning site Green=Tags(s)

MLHTEGHALLRAVGQGKLRLARLLLEGGAYVNEGDAQGETALMAACRARYDDPQNKARMVRYLLEQGADP
NIADRLGRTALMHACAGGGGAAVASLLLAHGADPSVRDHAGASALVHALDRGDRETLATLLDACKAKGTE
VIIITTDTSPSGTKKTRQYLNSPPSPGVEDPAPAPPSPGVCTSPSEVQLQTAGGGRGLLSPRAQEEEEKR
DIFEFPLPKPPDDPSPSEPLPKPPRHPPKPLKRLNSEPWGLVAPPQPVPPAEGRPGLERLAAEFNGLTLT
GRPRLSRRHSTEGPEDPPPWAEKVTGGGPLSRRNTAPEAQESGLPSGLRQKLSRMEPVELDTPGHFCPDS
PESSRLSLERRRYSASPLTLPPAGSVSSPRQSQESLPGAVSPLSGRRRSPGLLERRGSGTLLLDHISQTR
PGFLPPLNVSPHPPIPDIRPQPGGRAPSLPAPPPSGAPGSPRTKRKLVRRHSMQTEQIRLLGGFQSLGGP
GEPGR

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001024851
ORF Size 1485 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001024851.3, NP_001020022.2
RefSeq Size 2586 bp
RefSeq ORF 1488 bp
Locus ID 545554
Cytogenetics 3 F2.1
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Documents
Resources
"NM_001024851" in other vectors (4)
SKU Description Size Price
MC217090 Ankrd34a (untagged) - Mouse ankyrin repeat domain 34A (Ankrd34a), (10ug) 10 ug
$503.00
MR214346 Ankrd34a (Myc-DDK-tagged) - Mouse ankyrin repeat domain 34A (Ankrd34a) 10 ug
$289.00 $457.00
MR214346L3 Lenti ORF clone of Ankrd34a (Myc-DDK-tagged) - Mouse ankyrin repeat domain 34A (Ankrd34a) 10 ug
$757.00
MR214346L4 Lenti ORF clone of Ankrd34a (mGFP-tagged) - Mouse ankyrin repeat domain 34A (Ankrd34a) 10 ug
$757.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.