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Trip12 (NM_133975) Mouse Tagged ORF Clone

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SKU
MG214320
Trip12 (tGFP-tagged) - Mouse thyroid hormone receptor interactor 12 (Trip12), (10ug)
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ORF Plasmid:
tGFP DDK
  $1,871.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Trip12
Synonyms 1110036I07Rik; 6720416K24Rik; AA410158; Gtl6; TRIP-12
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG214320 representing NM_133975
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTCCAACCGGCCTAATAACAATCCAGGGGGGTCACTGCGACGTTCACAGAGGAACACTGCCGGGGCCC
AACCACAAGACGACTCAATAGGAGGAAGAAGCTGCAGTTCATCATCTGCTGTCATAGTTCCACAACCAGA
GGATCCAGACAGAGCCAATACTTCAGAAAGGCAGAAAACGGGGCAGGTGCCTAAAAAAGACAATTCTCGA
GGAGTGAAACGCAGTGCTAGTCCAGACTACAACAGGACCAATTCTCCTAGCTCTGCGAAAAAGCCCAGAG
CATTTCAGCATATTGAGTCTTTCTCAGAAACCAATAAGCCACATAGTAAGTCAAAGAAGAGACATTTAGA
CCAGGAGCAACAACTGAAATCTGCACAATTGCCGTCAACAAGCAAGGCTCACACCAGAAAGAGTGTGGCT
GCTGGTAGTTCACGGAATCAGAAAAGAAAGAGGACAGAGAGTTCTTGTGTTAAAAGTGGTTCTGGGTCCG
AATCAACTGGTGCCGAAGAGAGATCTGCAAAACCCATCAAGCTGGCTTCAAAATCAGCCACCTCAGCCAA
AGCTGGGTGTAGCACCATCACCGATTCTTCTTCTGCTGCCTCTACTTCCTCATCATCTTCAGCTATAGCC
TCGGCCTCTTCTACTGTACCAGCAGGTGCCAGGGTGAAACAAGGAAAAGACCAGAACAAGGCCAGGCGTT
CCCGTTCAGCATCCAGCCCTAGTCCCAGAAGAAGTAGCAGGGAAAAGGAACAGAGTAAAACTGGTGGCTC
TTCAAAATTTGATTGGGCTGCTCGCTTCAGCCCCAAAGTTAGCCTACCTAAAACAAAACTGTCTCTTCCA
GGGTCTTCTAAGTCAGAGACATCAAAACCTGGACCTTCTGGATTACAGGCCAAATTAGCAAGTTTAAGAA
AGTCCACCAAGAAACGCAGTGAGTCCCCACCTGCTGAGCTCCCCAGTTTGAGGAGGAGCACACGCCAAAA
GACCACGGGCTCCTGTGCTAGTACCAGTCGGCGAGGCTCTGGCCTGGGCAAGAGAGGAGCAGCTGAGGCC
CGGCGGCAAGAGAAAATGGCAGACCCTGAAAGCAACCAGGAGACAGTAAATTCCTCAGCTGCCCGGACAG
ATGAAGCTCCCCAAGGAGCTGCAGCCTCTAGTTCTGTTGCAGGGGCTGTTGGCATGACCACCTCTGGGGA
GAGTGAATCGGATGATTCTGAGATGGGACGATTACAAGCTCTGTTAGAGGCCAGGGGTCTTCCTCCTCAC
CTATTTGGTCCTCTCGGGCCTCGGATGTCACAACTTTTCCATAGAACAATTGGAAGCGGAGCTAGTTCTA
AGGCCCAGCAGCTTCTTCAAGGACTACAAGCCAGTGATGAAAGTCAACAGCTTCAGGCCGTCATTGAAAT
GTGTCAGTTATTGGTCATGGGGAATGAGGAGACACTAGGAGGGTTTCCTGTGAAGAGTGTTGTTCCAGCT
TTGATAACATTACTGCAGATGGAACATAATTTTGACATTATGAATCATGCTTGCCGAGCCTTAACATATA
TGATGGAAGCTCTCCCCCGGTCATCTGCTGTTGTAGTGGATGCTATCCCTGTTTTCTTAGAAAAGTTGCA
AGTTATTCAGTGTATTGATGTGGCAGAGCAGGCCTTGACTGCCTTGGAGATGTTGTCACGGCGACACAGT
AAAGCCATTTTACAAGCGGGTGGTTTGGCAGATTGCTTGTTGTATCTAGAATTCTTCAGCATCAATGCCC
AAAGAAATGCACTAGCAATTGCAGCCAATTGCTGCCAGAGTATCACACCAGATGAGTTTCATTTTGTGGC
AGATTCCCTACCATTGCTTACCCAAAGGCTAACTCATCAGGACAAAAAGTCAGTAGAAAGTACTTGCCTT
TGTTTTGCACGTTTAGTGGACAACTTTCAACATGAGGAGAACTTACTGCAACAGGTTGCTTCCAAAGATC
TACTTACAAATGTTCAACAATTGTTGGTAGTGACTCCACCGATTTTAAGTTCTGGGATGTTTATTATGGT
GGTTCGCATGTTTTCTCTGATGTGTTCCAACTGTCCAACATTAGCTGTTCAACTTATGAAACAAAACATT
GCAGAAACACTTCACTTCCTGCTATGTGGTGCCTCCAATGGAAGTTGTCAAGAGCAGATTGACCTTGTTC
CACGAAGTCCTCAAGAGCTGTATGAGCTGACATCTTTGATTTGTGAACTTATGCCATGTTTACCAAAAGA
AGGCATCTTCGCAGTTGATACCATGTTGAAAAAGGGAAATGCACAGAACACAGATGGTGCAATATGGCAG
TGGCGGGATGACCGAGGCCTCTGGCATCCCTATAACAGGATTGACAGCCGGATCATTGAGGCAGCCCATC
AGGTCGGTGAGGATGAGATAAGCTTGTCCACTCTGGGACGAGTTTATACTATTGATTTTAATTCTATGCA
GCAAATCAATGAAGACACGGGAACAGCACGTGCCATTCAGAGAAAACCTAATCCCTTAGCCAATTCTAAC
ACTAGTGGATATTCAGAGTTAAAGAAGGATGATGCTCGAGCACAGCTAATGAAAGAAGATCCGGAACTGG
CTAAGTCTTTCATTAAGACCTTGTTTGGTGTTCTCTATGAAGTGTACAGCTCCTCAGCAGGCCCTGCGGT
CAGACACAAGTGCCTTAGAGCAATTCTTAGGATAATTTATTTTGCTGATGCTGAACTTCTGAAGGATGTT
CTGAAAAATCATGCTGTTTCAAGTCACATTGCTTCAATGCTATCCAGCCAAGACCTGAAGATAGTAGTTG
GAGCTCTTCAGATGGCAGAAATCCTAATGCAAAAGTTACCTGATATTTTTAGTGTTTACTTCAGGAGAGA
AGGTGTGATGCATCAAGTCAAACATTTAGCAGAATCAGAGTCTTTGTTGACAAGTCCCCCCAAGGCATGT
ACAAATGGGTCAGGATCCCTGGGATCTACAACACCAGCCAGCAGTGGGACTGCAACGGCAGCAACCAACG
CCTCTGCTGATCTGGGGTCACCTAGCTTGCAGCATAGCAGAGATGATTCTTTGGATCTGAGCCCTCAAGG
TCGATTAAGTGATGTTCTCAAGAGAAAACGACTGCCAAAACGAGGGCCAAGAAGGCCAAAATATTCCCCT
CCAAGAGATGATGACAAAGTAGACAATCAAGCTAAAAGCCCCACCACTACTCAGTCACCTAAATCTTCCT
TCCTGGCAAGCTTGAATCCAAAAACATGGGGAAGGTTAAGCGCACAGTCCAACAGCAACAATATCGAACC
AGCACGAACTGCAGGAGTTAGTGGTCTTGCCAGGGCAGCCTCAAAAGACACCATATCCAATAATAGAGAA
AAAATTAAAGGCTGGATTAAGGAGCAGGCACATAAATTTGTTGAGCGTTATTTTAGTTCTGAGAATATGG
ATGGAAGCAACCCTGCCTTGAATGTCCTTCAGAGACTTTGTGCTGCAACTGAACAACTCAACCTCCAGGT
GGATGGTGGAGCTGAGTGCCTTGTAGAAATCCGTAGCATAGTCTCAGAGTCAGATGTTTCTTCATTTGAA
ATCCAACATAGTGGGTTTGTGAAACAACTGTTGCTGTATTTGACATCTAAGAATGAAAAGGATGCTGTCG
GCAGAGAGATCCGGTTAAAACGTTTCCTTCATGTATTTTTTTCTTCTCCACTTCCTGGAGAAGAGCCTGT
TGGAAGAGTAGAACCAGTGGGCCATGCACCTTTGCTGGCATTGGTTCACAAGATGAACAACTGTCTCAGC
CAAATGGAGCAATTTCCAGTCAAAGTACATGATTTCCCTAGTGGGAACGGAGCTGGAGGAAGCTTTTCTC
TCAACAGAGGATCACAAGCTTTAAAATTTTTCAACACACATCAGTTAAAATGTCAGTTACAAAGACACCC
AGACTGTGCAAATGTGAAGCAGTGGAAAGGCGGGCCTGTCAAGATTGATCCCCTGGCTCTGGTGCAAGCT
ATTGAGAGATACCTTGTGGTTAGAGGATATGGAAGAGTAAGAGAAGATGATGAAGATAGTGATGATGATG
GGTCAGATGAAGAAATAGATGAGTCTTTGGCTGCTCAATTCTTAAATTCGGGAAATGTAAGGCACAGGCT
ACAGTTTTATATTGGAGAACATTTACTACCATATAACATGACCGTGTATCAGGCAGTGCGGCAATTCAGT
GTACAGGCTGAAGATGAAAGAGAATCTACTGATGATGAGAGCAATCCTCTGGGAAGAGCTGGAATTTGGA
CAAAGACTCATACAATATGGTACAAACCTGTGAGGGAGGATGAAGAAAGTACTAAAGATTGTGTTGGTGG
CAAAAGGGGGAGAGCCCAAACAGCTCCAACAAAAACTTCCCCCAGAAATGCAAAGAAGCATGATGAGTTA
TGGCATGATGGAGTGTGCCCGTCAGTAGCAAATCCTTTAGAAGTTTACCTCATTCCCACACCACCTGAAA
ATATCACCTTTGAAGACCCATCCTTAGATGTAATACTTCTTTTAAGAGTTTTACATGCCATCAGTCGATA
TTGGTATTACTTGTATGATAATGCGATGTGCAAGGAGATTATTCCAACTAGTGAATTTATTAACAGTAAA
TTAACAGCAAAAGCAAATAGGCAGCTTCAAGACCCTCTAGTAATCATGACAGGAAACATCCCAACATGGC
TCACTGAGCTAGGAAAAACCTGCCCATTTTTCTTTCCTTTTGATACCCGACAAATGCTTTTTTATGTAAC
TGCATTTGATCGGGACCGGGCAATGCAAAGATTACTTGATACCAACCCAGAAATCAACCAGTCTGATTCT
CAAGACAGCAGAGTTGCGCCTAGATTGGATAGAAAAAAACGTACTGTGAACAGAGAGGAGCTACTGAAAC
AAGCTGAGTCTGTGATGCAGGACCTTGGCAGCTCTCGGGCCATGTTAGAAATCCAATATGAAAATGAGGT
TGGTACAGGTCTGGGGCCAACACTGGAGTTTTATGCACTTGTTTCTCAGGAACTACAGAGAGCTGACTTG
TGTCTCTGGAGGGGTGAAGAAGTAACTCTTAGCAATCCAAAAGGAAGCCAAGAAGGGACCAAGTATATTC
AAAACCTCCAGGGCCTGTTTGCGCTTCCCTTTGGTAGGACAGCTAAGCCAGCTCACATCGCAAAGGTTAA
GATGAAGTTTCGCTTCTTAGGAAAATTAATGGCTAAGGCTATCATGGATTTCAGATTGGTGGACCTTCCT
CTTGGCCTGCCCTTTTATAAGTGGATGCTGCGGCAGGAAACTTCATTGACATCTCATGATTTATTTGACA
TTGATCCTGTTGTAGCCAGATCAGTATATCACCTAGAAGACATTGTCAGACAGAAGAAAAGACTGGAGCA
GGATAAATCCCAGACCAAAGAGAGTCTACAATATGCATTGGAAACTCTTACGATGAATGGCTGTTCAGTG
GAAGATCTGGGATTGGATTTTACGCTGCCTGGGTTTCCCAACATTGAATTGAAGAAAGGAGGGAAGGATA
TTCCAGTTACTATCCACAATTTGGAAGAGTACCTGAGATTGGTGATATTCTGGGCACTAAATGAAGGTGT
TTGTCGACAGTTTGACTCATTCAGAGATGGATTTGAATCGGTCTTCCCGCTCTGCCATCTTCAGTACTTC
TACCCAGAAGAGCTGGACCAGCTCCTGTGTGGCAGTAAAGCAGACACTTGGGATGCAAAGACATTGATGG
AATGCTGCAGGCCTGATCATGGCTATACTCATGACAGTCGGGCTGTGAAGTTCTTGTTTGAGATTCTCAG
TAGTTTTGATAATGAGCAGCAGAGGTTGTTTCTCCAGTTTGTGACTGGTAGCCCACGATTGCCAGTTGGA
GGATTTCGGAGTTTAAATCCACCTCTGACAATTGTGCGGAAGACATTTGAATCAACAGAAAACCCAGATG
ACTTCTTACCATCAGTAATGACTTGTGTGAACTATCTTAAATTGCCGGACTACTCGAGCATTGACATAAT
GCGGGACAAACTCTTGATAGCAGCAAGAGAAGGCCAACAGTCGTTCCATCTTTCC

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG214320 representing NM_133975
Red=Cloning site Green=Tags(s)

MSNRPNNNPGGSLRRSQRNTAGAQPQDDSIGGRSCSSSSAVIVPQPEDPDRANTSERQKTGQVPKKDNSR
GVKRSASPDYNRTNSPSSAKKPRAFQHIESFSETNKPHSKSKKRHLDQEQQLKSAQLPSTSKAHTRKSVA
AGSSRNQKRKRTESSCVKSGSGSESTGAEERSAKPIKLASKSATSAKAGCSTITDSSSAASTSSSSSAIA
SASSTVPAGARVKQGKDQNKARRSRSASSPSPRRSSREKEQSKTGGSSKFDWAARFSPKVSLPKTKLSLP
GSSKSETSKPGPSGLQAKLASLRKSTKKRSESPPAELPSLRRSTRQKTTGSCASTSRRGSGLGKRGAAEA
RRQEKMADPESNQETVNSSAARTDEAPQGAAASSSVAGAVGMTTSGESESDDSEMGRLQALLEARGLPPH
LFGPLGPRMSQLFHRTIGSGASSKAQQLLQGLQASDESQQLQAVIEMCQLLVMGNEETLGGFPVKSVVPA
LITLLQMEHNFDIMNHACRALTYMMEALPRSSAVVVDAIPVFLEKLQVIQCIDVAEQALTALEMLSRRHS
KAILQAGGLADCLLYLEFFSINAQRNALAIAANCCQSITPDEFHFVADSLPLLTQRLTHQDKKSVESTCL
CFARLVDNFQHEENLLQQVASKDLLTNVQQLLVVTPPILSSGMFIMVVRMFSLMCSNCPTLAVQLMKQNI
AETLHFLLCGASNGSCQEQIDLVPRSPQELYELTSLICELMPCLPKEGIFAVDTMLKKGNAQNTDGAIWQ
WRDDRGLWHPYNRIDSRIIEAAHQVGEDEISLSTLGRVYTIDFNSMQQINEDTGTARAIQRKPNPLANSN
TSGYSELKKDDARAQLMKEDPELAKSFIKTLFGVLYEVYSSSAGPAVRHKCLRAILRIIYFADAELLKDV
LKNHAVSSHIASMLSSQDLKIVVGALQMAEILMQKLPDIFSVYFRREGVMHQVKHLAESESLLTSPPKAC
TNGSGSLGSTTPASSGTATAATNASADLGSPSLQHSRDDSLDLSPQGRLSDVLKRKRLPKRGPRRPKYSP
PRDDDKVDNQAKSPTTTQSPKSSFLASLNPKTWGRLSAQSNSNNIEPARTAGVSGLARAASKDTISNNRE
KIKGWIKEQAHKFVERYFSSENMDGSNPALNVLQRLCAATEQLNLQVDGGAECLVEIRSIVSESDVSSFE
IQHSGFVKQLLLYLTSKNEKDAVGREIRLKRFLHVFFSSPLPGEEPVGRVEPVGHAPLLALVHKMNNCLS
QMEQFPVKVHDFPSGNGAGGSFSLNRGSQALKFFNTHQLKCQLQRHPDCANVKQWKGGPVKIDPLALVQA
IERYLVVRGYGRVREDDEDSDDDGSDEEIDESLAAQFLNSGNVRHRLQFYIGEHLLPYNMTVYQAVRQFS
VQAEDERESTDDESNPLGRAGIWTKTHTIWYKPVREDEESTKDCVGGKRGRAQTAPTKTSPRNAKKHDEL
WHDGVCPSVANPLEVYLIPTPPENITFEDPSLDVILLLRVLHAISRYWYYLYDNAMCKEIIPTSEFINSK
LTAKANRQLQDPLVIMTGNIPTWLTELGKTCPFFFPFDTRQMLFYVTAFDRDRAMQRLLDTNPEINQSDS
QDSRVAPRLDRKKRTVNREELLKQAESVMQDLGSSRAMLEIQYENEVGTGLGPTLEFYALVSQELQRADL
CLWRGEEVTLSNPKGSQEGTKYIQNLQGLFALPFGRTAKPAHIAKVKMKFRFLGKLMAKAIMDFRLVDLP
LGLPFYKWMLRQETSLTSHDLFDIDPVVARSVYHLEDIVRQKKRLEQDKSQTKESLQYALETLTMNGCSV
EDLGLDFTLPGFPNIELKKGGKDIPVTIHNLEEYLRLVIFWALNEGVCRQFDSFRDGFESVFPLCHLQYF
YPEELDQLLCGSKADTWDAKTLMECCRPDHGYTHDSRAVKFLFEILSSFDNEQQRLFLQFVTGSPRLPVG
GFRSLNPPLTIVRKTFESTENPDDFLPSVMTCVNYLKLPDYSSIDIMRDKLLIAAREGQQSFHLS

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_133975
ORF Size 6075 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_133975.4, NP_598736.4
RefSeq Size 9632 bp
RefSeq ORF 6078 bp
Locus ID 14897
UniProt ID G5E870
Cytogenetics 1 C5
Summary E3 ubiquitin-protein ligase involved in ubiquitin fusion degradation (UFD) pathway and regulation of DNA repair. Part of the ubiquitin fusion degradation (UFD) pathway, a process that mediates ubiquitination of protein at their N-terminus, regardless of the presence of lysine residues in target proteins. In normal cells, mediates ubiquitination and degradation of isoform p19ARF/ARF of CDKN2A, a lysine-less tumor suppressor required for p53/TP53 activation under oncogenic stress. In cancer cells, however, isoform p19ARF/ARF and TRIP12 are located in different cell compartments, preventing isoform p19ARF/ARF ubiquitination and degradation. Does not mediate ubiquitination of isoform p16-INK4a of CDKN2A. Also catalyzes ubiquitination of NAE1 and SMARCE1, leading to their degradation. Ubiquitination and degradation of target proteins is regulated by interaction with proteins such as MYC, TRADD or SMARCC1, which disrupt the interaction between TRIP12 and target proteins. Acts as a key regulator of DNA damage response by acting as a suppressor of RNF168, an E3 ubiquitin-protein ligase that promotes accumulation of 'Lys-63'-linked histone H2A and H2AX at DNA damage sites, thereby acting as a guard against excessive spreading of ubiquitinated chromatin at damaged chromosomes.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_133975" in other vectors (2)
SKU Description Size Price
MC225130 Trip12 (untagged) - Mouse thyroid hormone receptor interactor 12 (Trip12), (10ug) 10 ug
$1,839.00
MR214320 Trip12 (Myc-DDK-tagged) - Mouse thyroid hormone receptor interactor 12 (Trip12) 10 ug
$1,671.00

file-alt Citations

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