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Spata31d1a (NM_028157) Mouse Tagged ORF Clone

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SKU
MG214314
Spata31d1a (tGFP-tagged) - Mouse RIKEN cDNA 1700013B16 gene (1700013B16Rik), (10ug)
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ORF Plasmid:
tGFP DDK
  $1,343.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Spata31d1a
Synonyms 1700013B16Rik; Fam75d1a; Fam75d3
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG214314 representing NM_028157
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGAAGATTCTCTCAGCTCTGAATGGCCTCAGTGAGACATGGCTGACCCTAGGCTCAGCTTCCTACC
ACATAGACCTCAACTACACTCTTCTGAGTGGACTGGGGCTACTGCTTCTGTACATCTCCTACCTGATATT
GAAACTGCTTATACATTTGCTGGGGAGAAAGAAATACACCCCAAAGCGGCAGAACAGCATCAGGCGCAAA
CAAAGGAAAGCATTTAAACGGAGGAAAGTTGGCCCGAGAGACCCTGAGGAAGGAAGGAGGCTGCAGACAG
TCCTACAAAGCCCAGACAAGCCATATGATCCCACGGGCTTTCGGCAAGCGCTATGCCCAGATCCCCGTTG
TCGCGTATGTAACAAAGCAAGCACTCAGGTCAGACACCTGCTCTCTCAGGCTCCCCTGGAAGATGGTGCT
GCCTCGGTGTCCTCTTTGCCATCTACCGCTCCCCTGATAGAGGCTTCGCTTGCTCTGCCCCCTCACTTTC
CTGCCAACCGACCAGGACACCCAGCTCCAGAAACTCCTCCCCACACTTCTCGCTCCATCCACTTGCAGAA
CCAAATCACTCCTTTGGATGGTGTATCTTCTCCCCCCATATCGCTCAGCGCCTCTCTGTCACCACAAGCT
GCTGCCTCCTTAAACCCTGCAGTGTTACCGGCCCACACCCCACGCAGAACACTTGCCTCTTTTCCACACC
TACATCCAAACGCCACTCAGGAAGCACAGTTAGCTCTCCAAGCAGAAGTTCCCCAGCCTCCCGTGGAAAG
CCCTAGGGAGCGGTCCCCCAGTGTCTCAACTGCCAAAAGCACTGTCTATGAGCCAGCATCCTCCCGGCCA
CAGTTCACTGCTGAAGACCAGTCCCTGACAAATTCGACACATGTTATTAACCATCAGCCTCCTCCATCAG
ACTCCTATGCTTCTCAGGGCTCATACCAGGGACTCACAAAGGCCTATGCTGTGGAGCCTGGGGATCATTT
GTCTCCCAGCCCCGATGTCTTAGCACTCTTCACGAAGCAAGAGGAAGGGGATGCTGATGGCACTGTGTAT
AAACTTAAAAGGGAGAAAGCAGTATCTTATATAATGCCATCAAATACTTCAGGGGAAATCTCAGCATCAA
CTGCTGGTCTAGAGAACTTGGAAGTCTCCCACCTCTTAGGTAATGAAAAGGGAAAGCCAGAGGACCCTCA
CCTACTCCAACAGCCCTCTCATCTGAAGAACTCTGAGGACCAGTCAGAGATAAAGGACATCCAGCTCTTC
TGGGGTCTCCCTTCTCTCCACAGTGAGTCCCTGAAACATGAGGCTGCCAACACAGGTAACAGTTACCCAG
GAAGGGACTATTTCAACAAAGTGGCTCAGGCCTCCACAGGCAGTCATTCTTCCAATCTTACTTACTCCAC
ACCTCTGCCCTTGCCTGAGAGTGACCAACAAGGGCAGATTGTACCTCAGTCTCAGATCATGCCTGACTCC
CTTGCCAACTCTAAGGCCCAGCCTCAATCCCTAGCCCAACTCCTGTCACCTTCTCCTCAGTCCCAGCTTG
GGATATGTGGGGTGCGTTTTCATAGCCTGAAGAATGAGGCACAGCCTCTCACCCCATGTGAAATTCAACA
GCTGGAATACAACCTATTGCAAAAAGCGCTGCAAAGTTTCTTGGGTTTACCCACTCTAACTGAAACATTC
CAAGAAAGCTTCTGTCCCCCACCTCCTAAAATCTCCTCAACCAGAAATTCATCCAAGGTCCATGCTCCCA
AGACAATCCTTCCTGGAGACTTCCCTCTCAGCCATACACTCAAGAGGAAACTCGAACACCATCTTCGAAA
GAGACTCATCCAGCGACACTGGGGCTTGCCCCACAGGATCCTTGAATCTCTGTCATTCTTGAGTCCTCAT
TCAGAAATTCCTGAGGCCCCCAAGCCAAGGGACACCCATGGACTCTCACGGGTCTCTCTCTATAAGTTTA
ATGCCAACACAAACCTACCAAGCTCTGGGGTAAGCCAATCGGAAAGATCCTGTAAGAAGCCCTTAGAGAG
TCACCCTCCACAGGAAAAAGACATCAACGTTCAGAGACACCGTCCAGGGATAAACCAAAGAGATCCCATA
CAATGTAAGTCCTGTGGAGCTACAAAGAGCACTGTACATTCTAATTCTGAGACAGACCCAGAACTTCAGC
TAGGGAATATGTCTGGAAACATTTCAAAGGCTTCATGGGTCAGACAGTGCCAGAAAGAATGTGAAGCTTA
CCTGCAAAAACATCTGGGCAAGGACACTAAAGAAATTAGTGGGAGTGAGAGGCCTAGTGCTGTGGACAGC
TCAAGGCATTCTCGGAACATGACACAGCCTCCACCTGAGACCTGCCCCAGACAGATGGAACCATTGGCAT
GTGAGGAGGATGCACTGACGAGGCACCCCCACAGCTTAGCGATTGGTCCTAGCAAAGAGAAGACGCTGGA
AGAACACATCACAGCCTTCCGCAGAAGGATGGCATTTGGCCTCCCAAAAAGGGTTGAGGAGTCCTTAGAA
TCAGACCTTACAAAAGCAGAGCCTTCTCAGCCTTTCCCTCAGCTCCACATTCAAGCCCACAACGTTTCTA
GAGCAGACCCTGCCAAGTCCTCCAGGTCCCTTCGGAGAAACACTACTGGAGATAGAATGGGGACAATGAC
TTCAGTCCCCACCCAACAGAGCCCTCTCCCTGCTGCCTCACTGGTGGGCCACAGCCAGCCAGCCTCTGAG
AACAAGAAGGTGGGTGTAGATAGAGACCTTAGCATAGCCCCAAGGGACAGAGAGCCAACCCAGCACTGGA
CACCCAGGAGGGCAGACAAAGGTGACGTGCAGCAGTCTGGATCACACAACAGACCTGGCCCAGAGCTGCC
CATGAGCCCAGGTGGGCCAACACATGAGAGATTGGCTTCCAGCACCAACACACAAGGGTCTCAGGAAGAA
AGGAGGAGCCGGGAAGACGGCTCCGAGGCCGAGGGCTCCACAGAGCTACACAAGGGAGAGCAACTCCCGG
GTCTTCACCCACAGTCCACTAAGAGCTTGAAAGGCACCCAAGGCCTGTGCTCCCCAGGGAGCCCTGTGAC
TGCATGTCAGTCCCCACAGGGAATGTCATTTCTCCACAACTCTGAGAGCCCTGACTCTAAAAGTCAGGTG
TCCACAGAAGTTCAGCTGAACTCAGAGGGCGGGACACACAACCAAGTGCCAGACCTGCCTGCCACGCCCT
TTGCCCCACAAGAAATGACTTCCAAACCCCAGGGCCCCTCCGGTGGGGACATGGCAGTTTCTCAGGTACT
GCGTGTGCACATGCCCACTGTGGGCATCAGCATGGAGTCGAGGCAGGGTCCCTGGGTCCCTGCATATGTC
TCAGGCAAGAGCAAGAACAAGGACTGTCTCCCTGCTGCCAGGGGATTGCCACAGCTGGCAACTGAGGCAG
GAAAGTTTGGGGGAGGGGATGCAGGCTTAGGGACATCCCAGACCACAGGGAAGGGACACTGTGTTCAGGC
CAGGGCACCAGAGGAGACACAAGGACTCACAGCCTCCCCAGCACTGACACCTAAGAGTCAGCCTCAGGAA
AATCAGTTCACCAGCCAGGTGAAGGGCTTCTGGCAGAGGCTGTCCCCTGGGAAGAAACACAAAGGACAGG
AAAAGTCCCTGGCCAAGGGCTGCTCTCCATTAGCATCTGGGAAGGGCACAAGCCCCATCAAAGGGAGAAG
TGAGTTTTGTGGGAACCCTGAAGCTCAGAACTGTGTGAGGGAGCCTGGGATGGTCCTGAGGAAGCAGCTG
GGGCACAGGCATGGGACAGTCACCCCCTGCCCCCAGGTCCCTGTGTCTCCCCTCATGGGGTCTGAAGGAG
CTCAGCAGGAGGTGCCCCTGCAGGCTCAGGCAGAGCCTGTCCAGAGGCTGCCCCACTTGTGCTGCAGAGG
TTCCTGCTCTCCAGTGCCAAGGGCTGAGTCCTGCAGCCCAGGACAGGGACAGACAGCCCCTGAGAGGTGT
GGCCCTACAGGAAAAAGC

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG214314 representing NM_028157
Red=Cloning site Green=Tags(s)

MEKILSALNGLSETWLTLGSASYHIDLNYTLLSGLGLLLLYISYLILKLLIHLLGRKKYTPKRQNSIRRK
QRKAFKRRKVGPRDPEEGRRLQTVLQSPDKPYDPTGFRQALCPDPRCRVCNKASTQVRHLLSQAPLEDGA
ASVSSLPSTAPLIEASLALPPHFPANRPGHPAPETPPHTSRSIHLQNQITPLDGVSSPPISLSASLSPQA
AASLNPAVLPAHTPRRTLASFPHLHPNATQEAQLALQAEVPQPPVESPRERSPSVSTAKSTVYEPASSRP
QFTAEDQSLTNSTHVINHQPPPSDSYASQGSYQGLTKAYAVEPGDHLSPSPDVLALFTKQEEGDADGTVY
KLKREKAVSYIMPSNTSGEISASTAGLENLEVSHLLGNEKGKPEDPHLLQQPSHLKNSEDQSEIKDIQLF
WGLPSLHSESLKHEAANTGNSYPGRDYFNKVAQASTGSHSSNLTYSTPLPLPESDQQGQIVPQSQIMPDS
LANSKAQPQSLAQLLSPSPQSQLGICGVRFHSLKNEAQPLTPCEIQQLEYNLLQKALQSFLGLPTLTETF
QESFCPPPPKISSTRNSSKVHAPKTILPGDFPLSHTLKRKLEHHLRKRLIQRHWGLPHRILESLSFLSPH
SEIPEAPKPRDTHGLSRVSLYKFNANTNLPSSGVSQSERSCKKPLESHPPQEKDINVQRHRPGINQRDPI
QCKSCGATKSTVHSNSETDPELQLGNMSGNISKASWVRQCQKECEAYLQKHLGKDTKEISGSERPSAVDS
SRHSRNMTQPPPETCPRQMEPLACEEDALTRHPHSLAIGPSKEKTLEEHITAFRRRMAFGLPKRVEESLE
SDLTKAEPSQPFPQLHIQAHNVSRADPAKSSRSLRRNTTGDRMGTMTSVPTQQSPLPAASLVGHSQPASE
NKKVGVDRDLSIAPRDREPTQHWTPRRADKGDVQQSGSHNRPGPELPMSPGGPTHERLASSTNTQGSQEE
RRSREDGSEAEGSTELHKGEQLPGLHPQSTKSLKGTQGLCSPGSPVTACQSPQGMSFLHNSESPDSKSQV
STEVQLNSEGGTHNQVPDLPATPFAPQEMTSKPQGPSGGDMAVSQVLRVHMPTVGISMESRQGPWVPAYV
SGKSKNKDCLPAARGLPQLATEAGKFGGGDAGLGTSQTTGKGHCVQARAPEETQGLTASPALTPKSQPQE
NQFTSQVKGFWQRLSPGKKHKGQEKSLAKGCSPLASGKGTSPIKGRSEFCGNPEAQNCVREPGMVLRKQL
GHRHGTVTPCPQVPVSPLMGSEGAQQEVPLQAQAEPVQRLPHLCCRGSCSPVPRAESCSPGQGQTAPERC
GPTGKS

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_028157
ORF Size 4008 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_028157.2, NP_082433.2
RefSeq Size 4281 bp
RefSeq ORF 4011 bp
Locus ID 72219
Cytogenetics 13 B2
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Documents
Resources
"NM_028157" in other vectors (4)
SKU Description Size Price
MC224265 Spata31d1a (untagged) - Mouse family with sequence similarity 75, member D3 (Fam75d3), (10ug) 10 ug
$1,258.00
MR214314 Spata31d1a (Myc-DDK-tagged) - Mouse family with sequence similarity 75, member D3 (Fam75d3) 10 ug
$589.00 $1,143.00
MR214314L3 Lenti ORF clone of Spata31d1a (Myc-DDK-tagged) - Mouse family with sequence similarity 75, member D3 (Fam75d3) 10 ug
$1,443.00
MR214314L4 Lenti ORF clone of Spata31d1a (mGFP-tagged) - Mouse family with sequence similarity 75, member D3 (Fam75d3) 10 ug
$1,443.00

file-alt Citations

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