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Gm15127 (NM_001114400) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG213968
Gm15127 (tGFP-tagged) - Mouse predicted gene 15127 (Gm15127), (10ug)
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ORF Plasmid:
tGFP DDK
  $703.00
3 Weeks*
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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Gm15127
Synonyms ENSMUSG00000072928; OTTMUSG00000019138
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG213968 representing NM_001114400
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGAACCATGAAGACGAAATTAGACAGCTGAGATATCTAGAAGCTGCTAATTCTGAAGAAGAATACA
ATGAGGAGAATGACATACAGTCAGAAAGCTCCAGAGGGCAAGCATTTTTCCCTGGACATCCGCGAGGGAG
AGGACACAGACGTGACAGTAGGGGAAGAAATGGCCATTTTTTCAGACAAAGATTTTCAGCAAATCATCCT
CTACTTCATCTGACAAGACAAGTCAACCTAGAAAGTCTAACAAGGAGGAATGGAAACCAATCAGAAGAGA
GACAGCTACAACAAGAAAGAATCCAATTGTTCTTCAGGAGTTTACATGGCCTCTCCCAATCTCATGACCA
CCACATTGGCAGATCTCATGGCCACTATTCCAGAAGATCACGTAATCGATTCAGGAGAGCTCCTGTTCAC
TCAGAGAGATCTCATGTCTACTCAGGTAGAACTAATAACTATACAGATAGACCACACCATGACCTCTCAG
ATAGACCACACAATCAACTCTCAGTGAGACCACATCCTGACCTCTCAGGTAGACCACTTCATGACTACTC
AGGTAGACCACATCAAAACCATGCAGTTAGGCTTCAACATGGCCACTCAGATAGATATCATCTTACCCAT
ACAGAGAGAGTTCTTCCTAGCCACTCAGGGATAGCTCATCCTGGCCACACAGAGAGAGATAATCAAGTCC
AAAGAGGGAGAGCTCATGATAACCAATCAGGGTTAGCTCATCACAACCATGATGGCAGATCTCATCATGA
CCACTCAGGGAGAGCTTATCATGGCCATGCAAGGAGAGCTCATAACAACCAGTCATGGAGATCTGATAAC
AACCAGTCATGGAGAGATCTTAACCAGTCATGGAGAGGTCGGAACAACCAGTCACAAAGAGGTCATGACA
ACCAGTCACAGAGAGGTCATGACAACCAGTCACGTAGAGGTCATGACAACCAGTCACAGAGAGGTCATGA
CAACCAGTCACAGAGAGGTCATAATGATCAGTCATGGAGAGCTCATAACATCCGGTCACGGAGAGATCGT
TATCACACCTCATTGAGATCTAGTAGCATCCAGTCATGGATGCTTCATGAAAGAGAAAGATACCATGGTC
CAAGAGGAAGAAATGGACCAAGGGATGCATCACCACCAGGATCTGAGCCAGAAATTGTCAGATCGTCTGC
AATAAGAGAAAGGACTGACTATTTATGGGTACAGGAACATCGAAGAAATGAGACAGTTGAACTGAATGTA
GGGCTAGAAGACGGCTACAGCAGTGCTGAAGAGAGTGATGAAAGTTTTGACAGACTAACCTGTCGCTCCA
TGCTAGATACACTATAC

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG213968 representing NM_001114400
Red=Cloning site Green=Tags(s)

MANHEDEIRQLRYLEAANSEEEYNEENDIQSESSRGQAFFPGHPRGRGHRRDSRGRNGHFFRQRFSANHP
LLHLTRQVNLESLTRRNGNQSEERQLQQERIQLFFRSLHGLSQSHDHHIGRSHGHYSRRSRNRFRRAPVH
SERSHVYSGRTNNYTDRPHHDLSDRPHNQLSVRPHPDLSGRPLHDYSGRPHQNHAVRLQHGHSDRYHLTH
TERVLPSHSGIAHPGHTERDNQVQRGRAHDNQSGLAHHNHDGRSHHDHSGRAYHGHARRAHNNQSWRSDN
NQSWRDLNQSWRGRNNQSQRGHDNQSQRGHDNQSRRGHDNQSQRGHDNQSQRGHNDQSWRAHNIRSRRDR
YHTSLRSSSIQSWMLHERERYHGPRGRNGPRDASPPGSEPEIVRSSAIRERTDYLWVQEHRRNETVELNV
GLEDGYSSAEESDESFDRLTCRSMLDTLY

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001114400
ORF Size 1347 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001114400.2, NP_001107872.2
RefSeq Size 1972 bp
RefSeq ORF 1350 bp
Locus ID 434866
Cytogenetics X F2
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"NM_001114400" in other vectors (2)
SKU Description Size Price
MC215760 Gm15127 (untagged) - Mouse predicted gene 15127 (Gm15127), (10ug) 10 ug
$503.00
MR213968 Gm15127 (Myc-DDK-tagged) - Mouse predicted gene 15127 (Gm15127) 10 ug
$503.00

Plasmid Map for MG213968

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MG213968
Circular map for MG213968

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