Nsmce4a (NM_001162855) Mouse Tagged ORF Clone
- TrueORF®
SKU
MG213222
Nsmce4a (tGFP-tagged) - Mouse non-SMC element 4 homolog A (S. cerevisiae) (Nsmce4a), (10ug)
$886.00
In Stock*
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
|---|---|
| Target Symbol | Nsmce4a |
| Synonyms | 2410003A14Rik |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_001162855 |
| ORF Size | 1143 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_001162855.1, NP_001156327.1 |
| RefSeq Size | 1484 bp |
| RefSeq ORF | 1146 bp |
| Locus ID | 67872 |
| Cytogenetics | 7 F3 |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_001162855" in other vectors (4)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| MC210642 | Nsmce4a (untagged) - Mouse non-SMC element 4 homolog A (S. cerevisiae) (Nsmce4a), (10ug) | 10 ug |
$732.00
|
|
| MR213222 | Nsmce4a (Myc-DDK-tagged) - Mouse non-SMC element 4 homolog A (S. cerevisiae) (Nsmce4a) | 10 ug |
$686.00
|
|
| MR213222L3 | Lenti ORF clone of Nsmce4a (Myc-DDK-tagged) - Mouse non-SMC element 4 homolog A (S. cerevisiae) (Nsmce4a) | 10 ug |
$986.00
|
|
| MR213222L4 | Lenti ORF clone of Nsmce4a (mGFP-tagged) - Mouse non-SMC element 4 homolog A (S. cerevisiae) (Nsmce4a) | 10 ug |
$986.00
|
|
Citations
Interaction between NSMCE4A and GPS1 links the SMC5/6 complex to the COP9 signalosome
Horváth, Rona, Pagano et al
BMC Mol Cell Biol (2020) 21 (1), 36 DOI: 10.1186/s12860-020-00278-x
Horváth, Rona, Pagano et al
BMC Mol Cell Biol (2020) 21 (1), 36 DOI: 10.1186/s12860-020-00278-x
Cited by 7
AI Summary: OriGene products contributed to this study by providing the FLAG-tagged mouse GPS1 cDNA vector (pCMV6-Entry DDK/Myc GPS1 transcript variant 1) and the mouse NSMCE4A cDNA vector (pCMV6-AC-GFP NSMCE4A), which were essential for generating constructs used to investigate the interaction between NSMCE4A and GPS1.
Snippet: … Methods Plasmids used in the study FLAG-tagged mouse GPS1 cDNA encoding vector was obtained from Origene (pCMV6-Entry DDK/Myc GPS1 transcript variant 1). Mouse NSMCE4A cDNA derived from the vector pCMV6-AC-GFP NSMCE4A (Origene) was cloned into the BamHI and XhoI sites of pCMV6-Entry vector (Orige;FLAG-tagged mouse GPS1 cDNA encoding vector was obtained from Origene (pCMV6-Entry DDK/Myc GPS1 transcript variant 1). Mouse NSMCE4A cDNA derived from the vector pCMV6-AC-GFP NSMCE4A (Origene) was cloned into the BamHI and XhoI sites of pCMV6-Entry vector (Origene) using BglII and XhoI. … NSMCE4A cDNA and GPS1 cDNA was cloned into this vector using the primer pairs (BamHI: 5′- cag gga tcc atg tct ggc gac agc ag − 3′;XhoI: 5′- cgg cac tcg atc tcc atg g − 3′) and (BamHI: 5′- cag gga tcc atg cgg ggc agc − 3′;NotI: 5′- gat gag ttt ctg ctc gag cg − 3′) respectively. …
Showing 1-1 of 1 papers.
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