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Tmem219 (NM_028389) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG212666
Tmem219 (tGFP-tagged) - Mouse transmembrane protein 219 (Tmem219) transcript variant 2, (10ug)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $500.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Tmem219
Synonyms 1110032O16Rik; 2900045G02Rik; IGFBP-3R
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG212666 representing NM_028389
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGCAGCTGCCAGGCAGGGCACAACTTGCATCTCTGTCTGGCTCATCACCCACCTTTGGTCTGTGCCA
CTTTGATCCTGCTGCTCCTTGGCCTTTCTGGCCTGGGCCTTGGTGGCTTCCTCCTCACCCACACAACTGG
CCTCCGTAGCCCTGACATTCCCCAGGATTGGGTTTCCTTCTTGAGATCTTTTGGCCAGCTAAGTCTGTGC
CCCATGAATGAGACAGTCACAGGGACGTGGCAAGGGCCTCACGTCGTCGGCTTACTGACCACTTTGAACT
TCGGAGATGGTCCAGACAGAAACAAAACCCAAACATTCCAAGCCAAGATCCATGGTAGTCAGATAGGATT
GACAGGGTCCTCTGCCGGAGAGTCTGTCCTTGTCACAGCCAGAGTGGCGTCAGGAAGGACTCCAGGGACC
TGCCTATATTTTAGTGGTGTTCCAAAAGTCCTGCCCTCTAGCCAACCACCTATATCCTGCTCAGAGGAGG
GAGTTGGAAATGCTACCCTGAGCCCAGTGATGGGTGAGGAGTGTGTCAGGGTCTGGAGCCACGAGCGCCT
TGTGCTCACCGAGCTCCTTACTTCAGAGGAGCTGGCTCTGTGTGGCAGCAGAGTGCTGGGCTTGGGCTTC
TTCCTGGTTCTGCTCTGTGGCCTCCTCTGCTGTACCACTGCAGTCTGCTTCCATCCGCGCCCAGAGTTCC
ACTGGTCTAGAACACGGCTT

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG212666 representing NM_028389
Red=Cloning site Green=Tags(s)

MGSCQAGHNLHLCLAHHPPLVCATLILLLLGLSGLGLGGFLLTHTTGLRSPDIPQDWVSFLRSFGQLSLC
PMNETVTGTWQGPHVVGLLTTLNFGDGPDRNKTQTFQAKIHGSQIGLTGSSAGESVLVTARVASGRTPGT
CLYFSGVPKVLPSSQPPISCSEEGVGNATLSPVMGEECVRVWSHERLVLTELLTSEELALCGSRVLGLGF
FLVLLCGLLCCTTAVCFHPRPEFHWSRTRL

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_028389
ORF Size 720 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_028389.1, NP_082665.1
RefSeq Size 959 bp
RefSeq ORF 723 bp
Locus ID 68742
UniProt ID Q9D123
Cytogenetics 7 F3
Summary Cell death receptor specific for IGFBP3, may mediate caspase-8-dependent apoptosis upon ligand binding.UniProtKB/Swiss-Prot Function
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"NM_028389" in other vectors (4)
SKU Description Size Price
MC210790 Tmem219 (untagged) - Mouse transmembrane protein 219 (Tmem219), transcript variant 2, (10ug) 10 ug
$300.00
MR212666 Tmem219 (Myc-DDK-tagged) - Mouse transmembrane protein 219 (Tmem219), transcript variant 2 10 ug
$289.00 $300.00
MR212666L3 Lenti ORF clone of Tmem219 (Myc-DDK-tagged) - Mouse transmembrane protein 219 (Tmem219), transcript variant 2 10 ug
$600.00
MR212666L4 Lenti ORF clone of Tmem219 (mGFP-tagged) - Mouse transmembrane protein 219 (Tmem219), transcript variant 2 10 ug
$600.00

Plasmid Map for MG212666

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MG212666
Circular map for MG212666

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