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Perm1 (NM_172417) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG212629
2310042D19Rik (tGFP-tagged) - Mouse RIKEN cDNA 2310042D19 gene (2310042D19Rik), (10ug)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $1,013.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Perm1
Synonyms 2310042D19Rik
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG212629 representing NM_172417
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGACAACTTCCAGTACAGCGTCCAGCTCAGCGATCGGGAGTGGGCCGAGTTTTCAGCCACTGCTGATG
AGTGTGGCCTCTTGCAGGCTGACCTGGCCTCTGGTGACGAGCCCTTGTCCAGTGACATTGACCAAGGGGA
CAGCAGTGGAAGCAGCCCCCCTGGGCCCCCACCCCTCTTCACTGGGCAGCTGGTTTCCCAAGGGAGGGGA
CAACAGAGCCGTGAGTTGGAGGACGTGGCTGCTCAGCAGCTGGTCAGCAGGTCTCAGTGTGAGCCTGTCC
TGGCTCTGGAGGCCAGTCATCAGGTAGCCGGCACGTCCACACAGTCAGAGGCCCCTCTGTTCCCCAGCTT
AGACAGTGTCTGTCCTGGCCAGGCCTTGTCCTTTCCAGGGCCGGCAACTTGCAGAGACAAGATGCAGAGG
CTTTTGCAAGGCCCTGCCCCTAGCTCTCCCAGTAAAGCCCCTCATAGTCCTGAGTCCCCTGGCCATAGCG
ACAACCCTCAGAGTTCCCCTGACAGCCTCGAAGCTTCACCGCGGAACCCTGGTCGTAAGAAGAGGCGTGC
TGTAGGTGCCAAGGGGACCAAGCACTCCGGATCTCTGGACTCTGCTGCTACCCAGATGAGCTCCCCACAG
CTTACCAGGCCCAAGGAGGCTCTCATGTCTGGGACACTGATGGCAAAAGCCCAGCAGGATAAGCCACAGC
CTGACTCCACGAGTCCTGAACAGATGGCCAGAGAGGAGTCAGGCTTGGATCTTTCTACACCTATTCTGAT
AACTGAACAAGATCAGATCAGAAAGACATCCAGAGCTGCGTTACATGTGGTATCCAAACCTGTCCAGGAA
GTTCACCCAGATGTCCCCATGGCCTCACCAAATGTGTCTACACGTGCCTCCAAGCCTCAACCCGACGTGG
CTTTGCCTAAACCTGCCTCCAAGCCGCAGTCTGACATAGCTTCGTCTACACATCCCTTCACGCCTGACGT
GTCTTTGTCCACACTTGCCTTCAAATGTCAACCCAATACAAATCAGTCTACACCCGCCTCTGAGCCTGAC
ATGGCTTTGTCTACACCTGCCTCTGAGCCTGACACGGCTTTGTCTACACCCGCCTCTGAGCCTGACACGG
CTTTGTCTACACCCGCCTCTGAGCCTGACACGGCTTTGTCTACACCCGCCTCTAGGTCGCAACTGGTGAA
GGCTGAATTTGCGTCTGCTTGCACACCTGGATTGCATGTGGACCTCTCTGCAGCAGGCTCAGAGATCAAG
CCAGAAGTTTCTTCCTCTATGCCTGCCGCAGTGGATGTGCTTCGTACAGATCTGCCTCGTTCTGTTTCAA
AGACTGAGTCTAAAGAGAGTGTGTCTATACCTGTTAAGCCTTCTTTATCCCCTATTTCCCAGGCTGAAGC
TGCCATGGTGGATACAGGTGTGTCTGTACCTCCAGGGGGAAGCATTGAGAAGCCAGCAGGGCAGTTCTCA
GCAGGGTCCTCAGGAGAGTCCTGCCTAGGTCCCGTCCAAGCCCCCAAGAAGAAGAAAGTACGATTTTCCA
TGGCTATCCCCAGCCATGAAGACTCAGGGTCAGGGGAGCCTACAGGTTCACCCTTCCTAACCCCGGAGCA
GCCCCCAGTTCCCAGGACAGCAGCAGGAAGCCGTGGGGGGTCTGCAGCCTGGGATGCTGTGGCTGTCGCG
CCCCGACTCCCCCAGCCTCGGATCCTCAAGCACCTGCCTCCTCCTGTATCCTCTGTATCAGCAGAGGCTG
AGTCTGGAAACTGCTTTGCAGTGACTCTCCCTGAGGCCTACGAGTTCTTCTTCTGTGACACCATTGAGGA
GGAAGATGAAGACGTAGAGGACGAAGAGGCAGCCAGCCAAGCCCTGGATGAAGTCCAATGGCCTGACACA
TGCGAGTTTTTTTTTCGGGATAGCAGAGCCCAAAGATCAAGATGTCAGAGGGGACACTCTCCAGTCTCAC
CACCAAGAGCAGACACCGTGGCACCTGTCCCCCCTGAAGGCTTGGTGCCTATTTCCATCCCTGAGGTCTA
TGAACACTTCTTCACTGAGGAAGGATTTGGGCACAGACAGCCTGCGGCCACTCCTATGCAGACCTCAGAG
CTTTCCAGGGAAGGAGGGCTTGAGGCATCCACCAAGCCTGTTTCACCTACAGCGGAACATCTTAGCTTAA
CAGTCAGGAAGGCAGGGGAACTACGGAGCCCCCTCACTTCATTTACGTTCAGTCAAAACGACATGTGCCT
GGTGTTTGTTGCCTTTGCCACTTGGGCTGTGAGAACCTCAGATCTACAAGCCCCAGATGCTTGGAAAACA
GTCTTGCTCGCCAACATTGGCACCATCTCTGCTATCCGCTACTTCCGCCGTCAGGTTGGGAGAGGCCATA
GTGGCAGACCGAGATCCAGTTCCAGCTCAAACCCCAGCTGC

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG212629 representing NM_172417
Red=Cloning site Green=Tags(s)

MDNFQYSVQLSDREWAEFSATADECGLLQADLASGDEPLSSDIDQGDSSGSSPPGPPPLFTGQLVSQGRG
QQSRELEDVAAQQLVSRSQCEPVLALEASHQVAGTSTQSEAPLFPSLDSVCPGQALSFPGPATCRDKMQR
LLQGPAPSSPSKAPHSPESPGHSDNPQSSPDSLEASPRNPGRKKRRAVGAKGTKHSGSLDSAATQMSSPQ
LTRPKEALMSGTLMAKAQQDKPQPDSTSPEQMAREESGLDLSTPILITEQDQIRKTSRAALHVVSKPVQE
VHPDVPMASPNVSTRASKPQPDVALPKPASKPQSDIASSTHPFTPDVSLSTLAFKCQPNTNQSTPASEPD
MALSTPASEPDTALSTPASEPDTALSTPASEPDTALSTPASRSQLVKAEFASACTPGLHVDLSAAGSEIK
PEVSSSMPAAVDVLRTDLPRSVSKTESKESVSIPVKPSLSPISQAEAAMVDTGVSVPPGGSIEKPAGQFS
AGSSGESCLGPVQAPKKKKVRFSMAIPSHEDSGSGEPTGSPFLTPEQPPVPRTAAGSRGGSAAWDAVAVA
PRLPQPRILKHLPPPVSSVSAEAESGNCFAVTLPEAYEFFFCDTIEEEDEDVEDEEAASQALDEVQWPDT
CEFFFRDSRAQRSRCQRGHSPVSPPRADTVAPVPPEGLVPISIPEVYEHFFTEEGFGHRQPAATPMQTSE
LSREGGLEASTKPVSPTAEHLSLTVRKAGELRSPLTSFTFSQNDMCLVFVAFATWAVRTSDLQAPDAWKT
VLLANIGTISAIRYFRRQVGRGHSGRPRSSSSSNPSC

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_172417
ORF Size 2421 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_172417.3, NP_766005.2
RefSeq Size 3849 bp
RefSeq ORF 2424 bp
Locus ID 74183
UniProt ID Q149B8
Cytogenetics 4 E2
Summary Regulates the expression of selective PPARGC1A/B and ESRRA/B/G target genes with roles in glucose and lipid metabolism, energy transfer, contractile function, muscle mitochondrial biogenesis and oxidative capacity. Required for the efficient induction of MT-CO2, MT-CO3, COX4I1, TFB1M, TFB2M, POLRMT and SIRT3 by PPARGC1A. Positively regulates the PPARGC1A/ESRRG-induced expression of CKMT2, TNNI3 and SLC2A4 and negatively regulates the PPARGC1A/ESRRG-induced expression of PDK4.UniProtKB/Swiss-Prot Function
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Resources
"NM_172417" in other vectors (4)
SKU Description Size Price
MC221766 2310042D19Rik (untagged) - Mouse RIKEN cDNA 2310042D19 gene (2310042D19Rik), (10ug) 10 ug
$814.00
MR212629 2310042D19Rik (Myc-DDK-tagged) - Mouse RIKEN cDNA 2310042D19 gene (2310042D19Rik) 10 ug
$813.00
MR212629L3 Lenti ORF clone of 2310042D19Rik (Myc-DDK-tagged) - Mouse RIKEN cDNA 2310042D19 gene (2310042D19Rik) 10 ug
$1,113.00
MR212629L4 Lenti ORF clone of 2310042D19Rik (mGFP-tagged) - Mouse RIKEN cDNA 2310042D19 gene (2310042D19Rik) 10 ug
$1,113.00

Plasmid Map for MG212629

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MG212629
Circular map for MG212629

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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