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Kcnk6 (NM_001033525) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG212215
Kcnk6 (tGFP-tagged) - Mouse potassium inwardly-rectifying channel subfamily K member 6 (Kcnk6), (10ug)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $500.00
3 Weeks*
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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Kcnk6
Synonyms D7Ertd764e; Toss; Twik2
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG212215 representing NM_001033525
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCGGCGGGGCGCGCTCCTGGCTAGCGCCCTGGCCGCCTACGCCGGGTACCTGGCGCTGGGCGCGCTGC
TTGTGGCGCGGCTGGAGCGGCCTCACGAAGCCCGGCTGCGGGCAGAACTGGGGACCCTGCGGGAGCAGCT
GCTACAGCACAGCCCGTGCGTGGCGGCTCACGCCCTGGATGCCTTCGTGGAGCGGGTGCTGGCGGCCGGA
CGGCTGGGGCGCGCAGCGCTCGCCAACGCCTCGGGAGCTGCCAACGCTTCGGACCCTGCGTGGGACTTCG
CCTCGGCGCTCTTCTTCGCCAGCACGCTTGTCACCACCGTGGGCTATGGCTACACGACCCCACTGACAGA
TGCTGGCAAAGCCTTCTCCATCGTCTTTGCACTCCTGGGCGTGCCTATCACCATGCTACTTCTGACTGCC
TCAGCCCAGCGCCTGTCACTGCTGCTCACCCACGCACCCCTGTCCTGGCTGAGCCTGCACTGGGGCTGGC
CCCCGCAGCGGGCAGCCCGCTGGCACCTGGTGGCCCTGCTGATGGTCATAGTGGCTATATTCTTCCTGGT
ACCGGCCGCGGTCTTCGCCTACCTTGAAGAGGCCTGGAGTTTTCTGGATGCTTTCTATTTCTGCTTCATC
TCTCTGTCCACCATTGGCCTAGGGGACTACGTGCCTGGGGAAGCCCCAGGCCAGCCTTACCGGGCCCTCT
ACAAGGTGCTTGTCACAGCGTACCTCTTCCTCGGCCTGGTTGCCATGGTGCTCGTGTTGCAGACTTTCCG
CCGTGTTTCCGACCTCCATGGCCTCACAGAACTCATCTTGCTGCCCGATCCGGACCCTGCCAGCCTCAGC
CAGGATGAGGATGATCAGGTGGCCGTTCTGGATGCCCGGACAGATCTACATCAGCACCTCTCTGCTGCCT
CCCACGCTGACTACGCCTCCATCCCCAGG

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG212215 representing NM_001033525
Red=Cloning site Green=Tags(s)

MRRGALLASALAAYAGYLALGALLVARLERPHEARLRAELGTLREQLLQHSPCVAAHALDAFVERVLAAG
RLGRAALANASGAANASDPAWDFASALFFASTLVTTVGYGYTTPLTDAGKAFSIVFALLGVPITMLLLTA
SAQRLSLLLTHAPLSWLSLHWGWPPQRAARWHLVALLMVIVAIFFLVPAAVFAYLEEAWSFLDAFYFCFI
SLSTIGLGDYVPGEAPGQPYRALYKVLVTAYLFLGLVAMVLVLQTFRRVSDLHGLTELILLPDPDPASLS
QDEDDQVAVLDARTDLHQHLSAASHADYASIPR

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001033525
ORF Size 939 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001033525.3, NP_001028697.2
RefSeq Size 4089 bp
RefSeq ORF 942 bp
Locus ID 52150
Cytogenetics 7 16.94 cM
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Documents
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"NM_001033525" in other vectors (4)
SKU Description Size Price
MC209891 Kcnk6 (untagged) - Mouse potassium inwardly-rectifying channel, subfamily K, member 6 (Kcnk6), (10ug) 10 ug
$330.00
MR212215 Kcnk6 (Myc-DDK-tagged) - Mouse potassium inwardly-rectifying channel, subfamily K, member 6 (Kcnk6) 10 ug
$289.00 $300.00
MR212215L3 Lenti ORF clone of Kcnk6 (Myc-DDK-tagged) - Mouse potassium inwardly-rectifying channel, subfamily K, member 6 (Kcnk6) 10 ug
$600.00
MR212215L4 Lenti ORF clone of Kcnk6 (mGFP-tagged) - Mouse potassium inwardly-rectifying channel, subfamily K, member 6 (Kcnk6) 10 ug
$600.00

Plasmid Map for MG212215

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MG212215
Circular map for MG212215

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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