Ephb4 (NM_010144) Mouse Tagged ORF Clone

SKU MG211390

Ephb4 (tGFP-tagged) - Mouse Eph receptor B4 (Ephb4)

Product Data

• Type: Mouse Tagged ORF Clone
• Target Symbol: Ephb4
• Synonyms: AI042935; Htk; MDK2; Myk1; Tyro11
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG211390 representing NM_010144; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGGAGCTCCGAGCGCTGCTGTGCTGGGCTTCCCTCGCCACTGCTTTAGAAGAGACCCTGTTGAACACAA; AACTGGAAACGGCGGATCTGAAATGGGTGACTTACCCTCAGGCAGAGGGCCAGTGGGAGGAGCTAAGCGG; CCTGGATGAGGAACAGCACAGCGTCCGCACCTATGAGGTGTGCGACATGAAGCGTCCAGGGGGCCAGGCT; CACTGGCTGCGCACTGGCTGGGTCCCAAGGCGAGGTGCTGTCCACGTGTATGCCACGATACGCTTCACCA; TGATGGAATGCCTGTCCCTGCCGAGGGCCAGTCGCTCCTGCAAGGAGACATTCACTGTCTTCTATTACGA; GAGCGAAGCTGATACGGCCACGGCCCATACGCCCGCCTGGATGGAGAACCCCTACATCAAGGTGGACACA; GTGGCCGCAGAACATCTGACTCGGAAGCGCCCTGGAGCTGAAGCCACAGGGAAAGTTAATATCAAGACGC; TGCGCCTGGGTCCTCTCAGCAAAGCTGGCTTCTACCTGGCTTTCCAGGACCAAGGAGCCTGCATGGCTCT; GCTCTCCCTGCATCTCTTTTACAAGAAGTGCTCCTGGCTGATCACGAACTTGACCTACTTCCCCGAGACG; GTGCCTCGGGAGCTCGTGGTGCCGGTGGCAGGTAGCTGCGTGGCCAACGCGGTCCCTACCGCCAACCCCA; GCCCCAGCCTCTACTGCCGGGAAGATGGTCAATGGGCTGAGCAGCAGGTCACGGGCTGCAGCTGCGCGCC; AGGGTACGAGGCTGCGGAAAGCAACAAAGTATGCAGAGCCTGTGGCCAGGGAACCTTCAAGCCCCAAATA; GGAGACGAGTCCTGCCTGCCATGCCCAGCCAACAGCCACTCGAATAACATTGGGTCTCCTGTCTGCCTGT; GTCGAATTGGGTATTACCGGGCCCGCTCAGACCCCCGGAGTTCACCTTGCACTACCCCACCCTCTGCTCC; AAGAAGCGTGGTTCACCATTTGAATGGTTCCACCCTGCGCCTGGAATGGAGTGCTCCCCTTGAGTCCGGA; GGCCGAGAGGACCTCACTTATGCTGTACGCTGCCGAGAGTGCCGTCCTGGGGGTTCCTGCTTGCCCTGTG; GGGGCGACATGACCTTCGACCCCGGTCCTCGAGACCTGGTTGAGCCCTGGGTGGCAATCCGAGGGCTGCG; TCCTGATGTCACCTATACCTTTGAGGTTGCTGCTTTGAATGGTGTGTCTACCTTAGCCACTGGACCACCT; CCTTTTGAGCCTGTCAATGTCACCACTGACCGTGAGGTGCCTCCTGCAGTGTCTGACATCCGAGTGACTC; GGTCGTCACCCAGCAGCTTGATCCTGTCATGGGCTATCCCCAGAGCACCCAGTGGGGCCGTGCTGGACTA; CGAGGTCAAGTATCATGAGAAGGGCGCAGAGGGCCCCAGCAGTGTTCGTTTCCTGAAGACATCAGAAAAC; CGAGCTGAGCTCCGGGGGCTGAAGCGGGGAGCCAGCTATCTGGTCCAGGTACGCGCACGGTCCGAGGCTG; GCTACGGTCCCTTCGGCCAGGAGCATCACAGTCAGACTCAACTGGATGAGAGCGAGAGCTGGCGGGAGCA; GCTGGCCCTGATTGCAGGCACTGCGGTTGTGGGTGTGGTCCTGGTCCTGGTGGTCGTCATCATTGCAGTT; CTCTGCCTCAGGAAGCAGAGCAATGGGAGGGAAGTTGAGTACTCGGATAAGCATGGGCAGTATCTCATCG; GGCACGGTACCAAGGTCTACATTGATCCTTTTACTTACGAAGACCCTAATGAGGCAGTGAGGGAATTTGC; CAAAGAGATCGATGTCTCCTATGTCAAGATTGAAGAGGTAATTGGTGCAGGTGAGTTCGGCGAGGTGTGC; CGGGGTCGGCTGAAGGCACCAGGGAAAAAGGAGAGCTGTGTGGCCATCAAGACTCTGAAGGGTGGCTACA; CCGAGCGCCAGAGGCGTGAGTTCCTGAGCGAGGCCTCCATCATGGGCCAGTTCGAGCATCCCAACATCAT; CCGCCTCGAGGGCGTGGTCACCAACAGTGTGCCGGTTATGATCCTCACGGAATTCATGGAGAACGGAGCC; CTGGACTCCTTCCTGCGGCTGAACGACGGGCAGTTCACAGTCATCCAGCTGGTGGGCATGCTGAGGGGCA; TCGCCTCGGGCATGCGGTACCTGGCTGAAATGAGCTATGTCCACCGAGACCTGGCTGCTCGGAACATCTT; GGTCAACAGTAACCTGGTCTGCAAGGTGTCCGACTTTGGCCTCTCCAGATTCTTGGAGGAGAACTCCTCT; GATCCCACCTACACAAGTTCCCTGGGAGGCAAGATTCCCATCCGATGGACCGCCCCTGAAGCCATTGCCT; TCAGGAAGTTCACCTCTGCCAGTGATGCCTGGAGCTATGGGATCGTCATGTGGGAGGTCATGTCTTTTGG; GGAACGGCCATACTGGGACATGAGCAACCAGGATGTGATCAATGCCATTGAACAGGACTACCGGCTGCCT; CCTCCTCCAGACTGCCCCACCTCCCTCCACCAGCTCATGCTGGATTGTTGGCAGAAGGACCGGAATGCCC; GGCCCCGCTTTCCCCAGGTGGTCAGCGCTCTGGACAAGATGATCCGGAATCCCGCTAGCCTCAAAATCGT; GGCCAGGGAGAATGGCGGGGCCTCACATCCACTCTTGGACCAACGGCAGCCTCACTATTCTGCTTTCGGT; TCTGTGGGCGAGTGGCTTCGAGCCATCAAGATGGGAAGATACGAGGAAAGTTTTGCAGCGGCTGGATTCG; GCTCCTTTGAGGTGGTCAGTCAGATCTCTGCCGAGGACCTTCTCCGAATTGGAGTCACTCTGGCAGGACA; CCAGAAGAAAATCTTGGCCAGTGTGCAGCATATGAAGTCCCAAGCTAAGCCAGGAGCCCCTGGTGGGACA; GGGGGACCAGCCCAGCAGTTC; ; ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG211390 representing NM_010144; Red=Cloning site Green=Tags(s); ; MELRALLCWASLATALEETLLNTKLETADLKWVTYPQAEGQWEELSGLDEEQHSVRTYEVCDMKRPGGQA; HWLRTGWVPRRGAVHVYATIRFTMMECLSLPRASRSCKETFTVFYYESEADTATAHTPAWMENPYIKVDT; VAAEHLTRKRPGAEATGKVNIKTLRLGPLSKAGFYLAFQDQGACMALLSLHLFYKKCSWLITNLTYFPET; VPRELVVPVAGSCVANAVPTANPSPSLYCREDGQWAEQQVTGCSCAPGYEAAESNKVCRACGQGTFKPQI; GDESCLPCPANSHSNNIGSPVCLCRIGYYRARSDPRSSPCTTPPSAPRSVVHHLNGSTLRLEWSAPLESG; GREDLTYAVRCRECRPGGSCLPCGGDMTFDPGPRDLVEPWVAIRGLRPDVTYTFEVAALNGVSTLATGPP; PFEPVNVTTDREVPPAVSDIRVTRSSPSSLILSWAIPRAPSGAVLDYEVKYHEKGAEGPSSVRFLKTSEN; RAELRGLKRGASYLVQVRARSEAGYGPFGQEHHSQTQLDESESWREQLALIAGTAVVGVVLVLVVVIIAV; LCLRKQSNGREVEYSDKHGQYLIGHGTKVYIDPFTYEDPNEAVREFAKEIDVSYVKIEEVIGAGEFGEVC; RGRLKAPGKKESCVAIKTLKGGYTERQRREFLSEASIMGQFEHPNIIRLEGVVTNSVPVMILTEFMENGA; LDSFLRLNDGQFTVIQLVGMLRGIASGMRYLAEMSYVHRDLAARNILVNSNLVCKVSDFGLSRFLEENSS; DPTYTSSLGGKIPIRWTAPEAIAFRKFTSASDAWSYGIVMWEVMSFGERPYWDMSNQDVINAIEQDYRLP; PPPDCPTSLHQLMLDCWQKDRNARPRFPQVVSALDKMIRNPASLKIVARENGGASHPLLDQRQPHYSAFG; SVGEWLRAIKMGRYEESFAAAGFGSFEVVSQISAEDLLRIGVTLAGHQKKILASVQHMKSQAKPGAPGGT; GGPAQQF; ; TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_010144
• ORF Size: 2961 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_010144.6, NP_034274.4
• RefSeq Size: 4334 bp
• RefSeq ORF: 2964 bp
• Locus ID: 13846
• UniProt ID: P54761
• Cytogenetics: 5 G2
• Summary: Receptor tyrosine kinase which binds promiscuously transmembrane ephrin-B family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Together with its cognate ligand/functional ligand EFNB2 it is involved in the regulation of cell adhesion and migration, and plays a central role in heart morphogenesis, angiogenesis and blood vessel remodeling and permeability. EPHB4-mediated forward signaling controls cellular repulsion and segregation from EFNB2-expressing cells.[UniProtKB/Swiss-Prot Function]